Development of hydrophobic tag purifying monophosphorylated RNA for chemical synthesis of capped mRNA and enzymatic synthesis of circular mRNA.

We developed phosphorylation reagents with a nitrobenzyl hydrophobic tag and used them for 5'-phosphorylation of chemically or transcriptionally synthesized RNA. The capability of hydrophobic tags to synthesize 5'-monophosphorylated RNA was evaluated based on the yield of the desired oligonucleotides, stability of protecting groups during cleavage/deprotection, separation ability in reverse-phase HPLC (RP-HPLC), and deprotection efficiency after RP-HPLC purification. The results showed that a nitrobenzyl derivative with a tert-butyl group at the benzyl position was most suitable for RNA 5'-phosphorylation.

Detection of Antibiotic Resistance in Feline-Origin ESBL from Different Areas of China and the Resistance Elimination of Garlic Oil to Cefquinome on ESBL .

The development of drug-resistance in the opportunistic pathogen has become a global public health concern. Due to the share of similar flora between pets and their owners, the detection of pet-origin antibiotic-resistant is necessary. This study aimed to detect the prevalence of feline-origin ESBL in China and to explore the resistance elimination effect of garlic oil to cefquinome on ESBL .

Detection of antibiotic-resistant canine origin and the synergistic effect of magnolol in reducing the resistance of multidrug-resistant .

Background: The development of antimicrobial resistance in the opportunistic pathogen has become a global public health concern. Due to daily close contact, dogs kept as pets share the same with their owners. Therefore, the detection of antimicrobial resistance in canine is important, as the results could provide guidance for the future use of antibiotics.

Fabrication of ternary UiO-66(Ce)/Ag/BiOBr heterojunction for enhanced photocatalytic degradation of ketoprofen via effective electron transfer process: Pathways, DFT calculation and mechanism.

Photocatalytic oxidation technique is considered as one of the most prospective approaches to solve the problem of environmental pollution. Herein, the novel ternary nanocomposite UiO-66(Ce)/Ag/BiOBr was fabricated via simple synthetic strategy. The obtained UiO-66(Ce)/Ag/BiOBr exhibited an excellent performance and photocatalytic efficiency of ketoprofen reached 93.

Biomass-derived porous material synthesized by one-step calcination method for the magnetic solid-phase extraction of polychlorinated biphenyls in water.

Recent findings unfold that biomass materials with the micro/mesoporous structure were often treated as adsorbents for organic substances. In this work, a one-step calcination method was adopted in the preparation of magnetic porous green bean biomass material. It has the properties of magnetism and porosity after the addition of Co(NO ) and high-temperature calcination.

Fast degradation of phthalate acid esters by polyoxometalate nanocatalysts through adsorption, esterolysis and oxidation.

A novel route was created to facilitate the degradation of diethyl phthalate (DEP) upon micellar polyoxometalate (POM) catalysts and HO. The best catalytic activity was obtained using [CHN(CH)]HPMoVO (N-hexadecyl-N,N,N-trimethylammonium tetrahydrogen decamolybdo-divanadophosphate, abbreviated as (CTA)HPMoV) with 90.2% degradation efficiency within 30 min, while the chemical oxygen demand (COD) and total organic carbon (TOC) removal efficiency were about 77.