Highly Efficient Analysis on Biomass Carbohydrate Mixtures by DREAMTIME NMR Spectroscopy.

Proton (H) NMR spectroscopy presents a powerful tool for biomass mixture studies by revealing the involved chemical compounds with identified ingredients and molecular structures. However, conventional H NMR generally suffers from spectral congestion when measuring biomass mixtures, particularly biomass carbohydrate samples, that contain various physically and chemically similar compounds. In this study, a targeted detection NMR approach, DREAMTIME, is exploited for studying biomass carbohydrate mixtures by spectroscopically targeting the desired compounds in separate 1D NMR spectra.

Robust 1D selective correlation NMR spectroscopy for rapid chemical and biological applications.

Selective homonuclear proton correlation NMR spectroscopy (COSY) provides a useful detection tool for elucidating molecular structures and identifying chemical compositions in 1D spectroscopic patterns. However, conventional 1D selective COSY experiments highly rely on the performance of selective excitation on targeted signals and their applications generally suffer from spectral congestion in complex chemical and biological samples. Herein, based on the concept of targeted excitation on coupled proton pairs and spectroscopic separation on their respective COSY responses, we propose a 1D selective NMR approach that is capable of individually recording direct coupling correlation information of targeted proton groups for analyses on complex samples, free of spectral congestion.

Pure-Shift-Based Proton Magnetic Resonance Spectroscopy for High-Resolution Studies of Biological Samples.

Proton magnetic resonance spectroscopy (H MRS) presents a powerful tool for revealing molecular-level metabolite information, complementary to the anatomical insight delivered by magnetic resonance imaging (MRI), thus playing a significant role in in vivo/in vitro biological studies. However, its further applications are generally confined by spectral congestion caused by numerous biological metabolites contained within the limited proton frequency range. Herein, we propose a pure-shift-based H localized MRS method as a proof of concept for high-resolution studies of biological samples.

[Comparison of Plasma Components between Frozen Plasma and Fresh Frozen Plasma].

Objective: To compare the plasma components of frozen plasma (FP) and fresh frozen plasma (FFP).

Methods: Twenty samples of FP and 20 samples of FFP from Beijing Red Cross Blood Center were randomly selected. Immediately after plasma melting, 12 plasma components including coagulation factor, fibrinolytic system and anticoagulation protein were detected, including activated partial thromboplastin time (APTT), prothrombin time (PT), coagulation factor Ⅷ (FⅧ) activity, coagulation factor Ⅴ (FⅤ) activity, fibrinogen(FIB) level, ADAMTS-13 activity, von Willebrand factor(vWF) activity, D-dimer (D-dimer, DD), fibrin degradation products (FDP), antithrombin (AT), protein C (PC), and protein S (PS).

[Analysis of the Irregular Antibodies of the Patients and Its Clinical Significance].

Objective: To retrospectively analyze the identification results of irregular antibodies, to clarify the distribution features and to explore the relation of alloantibodies and autoantibodies with the immunized history of patients and disease kinds.

Methods: 49 820 patients who applied for red blood transfusion during Sep 1st 2017 to Sep 1st 2018 were selected. All the specimens were screened for the antibody by microcolumn gel antiglobulin technique, which then were identified for irregular antibody.