FUNDC1 Mediated Mitophagy in Epileptic Hippocampal Neuronal Injury Induced by Magnesium-Free Fluid.

Mitophagy plays a key role in epileptic neuronal injury, and recent studies have shown that FUNDC1 plays an important role in regulating mitophagy. However, the specific effect of FUNDC1 on neuronal damage in epilepsy is unknown. In this study, we investigated the role of FUNDC1 in mitophagy and neuronal apoptosis using a hippocampal neuronal culture model of acquired epilepsy (AE) in vitro.

SV40 intron, a potent strong intron element that effectively increases transgene expression in transfected Chinese hamster ovary cells.

Chinese hamster ovary (CHO) cells have become the most widely utilized mammalian cell line for the production of recombinant proteins. However, the product yield and transgene instability need to be further increased and solved. In this study, we investigated the effect of five different introns on transgene expression in CHO cells.

Development and characterizations of a miniature capillary electrophoresis mass spectrometry system.

A miniature capillary electrophoresis mass spectrometry (CE/MS) system has been developed in this work. A 100% electrical driven miniaturized CE device was integrated with a miniature MS instrument, which has a discontinuous atmospheric pressure interface (DAPI) for coupling with atmospheric pressure ionization sources. A nanoelectrospray ionization (nano-ESI) source was developed with a sheath liquid interface for coupling the miniature CE and the MS system.

[In vitro effect of alendronate on chondrocytes and articular cartilage and subchondral bone in rabbit anterior cruciate ligament transection model].

Objective: To examine the effects of alendronate (ALN) on IL-1beta-stimulated chondrocyte of rabbit in vitro and on cartilage and subchondral bone in rabbit osteoarthritis (OA) induced by anterior cruciate ligament transection (ACLT).

Methods: The chondrocytes from articular surface of healthy 3-month-old Japanese White rabbits were obtained by the method of enzyme digestion and cultured in vitro. The third generation chondrocytes were assigned into three groups: the chondrocytes were cultured in DMEM medium with 10 ng/mL IL-1beta for 2 days, subsequently with (ALN group, group A1) or without (IL-1beta group, group B1) 1 x 10(-6) mol/L ALN for 3 days; the chondrocytes in vacant group (group C1) were cultured in DMEM medium for 5 days.

[Effect of calcitonin on articular cartilage of osteoarthritis].

Objective: To review the effect of calcitonin on cartilage and subchondral bone of osteoarthritis.

Methods: Recent literatures about the effect of calcitonin on osteoarthritis was reviewed.

Results: Calcitonin could promote the synthesis of important cartilage matrix such as proteoglycans and collagen II, propelling the regeneration of cartilage and subchondral bone.