Simultaneous determination of methyltestosterone and its metabolite in fish by gas chromatography-mass spectrometry.

Methyltestosterone is one of the banned drugs in aquaculture, and it should be monitored in food-producing animals. 17α-Methyl-5β-androstane-3α,17β-diol, as the main metabolite of methyltestosterone in vertebrates, could be used as another marker for controlling the administration of methyltestosterone, due to its high residual concentration and slow elimination rate. In this study, an analytical method based on gas chromatography-mass spectrometry (GC-MS) was developed and validated for the simultaneous determination of methyltestosterone and its main metabolite in fish.

In-vial solid-phase extraction of polycyclic aromatic hydrocarbons in drug formulations stored in packaging containing rubber.

Polycyclic aromatic hydrocarbons (PAHs) are a class of ubiquitous and persistent organic compounds that are significantly teratogenic, carcinogenic and mutagenic. Rubber stoppers commonly used in sterile formulation packaging materials often contain carbon black as the additive to enhance mechanical strength. However, PAHs may be formed during the production of carbon black, which could cause the drug formulations to be contaminated when contacting with the rubber stopper, and then enter the patient's body.