An expression profile of human alpha-lactalbumin in the milk of transgenic mouse.

Five female transgenic mice were produced by microinjection using a construct made up of a 7.3-kb-5' flanking region and a 2.0-kb coding region of human alpha-lactalbumin, as well as a 227-bp 3'-flanking region from bovine growth hormone gene.

Role of nucleostemin in growth regulation of gastric cancer, liver cancer and other malignancies.

Aim: To examine the role of nucleostemin in the growth regulation of gastric cancer, liver cancer and other cancers.

Methods: RT-PCR was used to clone the fragment of nucleostemin cDNA from HEK 293 cells. Eighteen kinds of malignant tumor tissues including gastric adenocarcinoma and liver cancer tissues, 3 kinds of benign tumor tissues, 3 kinds of benign hyperplastic tissues and normal tissues were employed to examine nucleostemin gene expression by RT-PCR, Slot blot, Northern blot and in situ hybridization.

Effect of human osteopontin on proliferation, transmigration and expression of MMP-2 and MMP-9 in osteosarcoma cells.

Background: To explore the effect of human osteopontin (hOPN) on the proliferation, transmigration and expression of matrix metallproteinase-2 (MMP-2) and matrix metallproteinase-9 (MMP-9) in osteosarcoma (OS) cells in vitro.

Methods: The prokaryotic-expression vector of hOPN was produced. hOPN was then subcloned into E.

Multiple cis-acting sequences implicate function diversity in nuclear matrix attachment regions of bovine mammary gland.

Chromosomal DNA in higher eukaryotes is spatially organized into loops by periodic attachment to the nuclear matrix at its base via a specific matrix attachment region (MAR). In order to study the nature of DNA sequences that affixed the loops to the nuclear matrix, we have cloned the MAR DNA from bovine lactating mammary tissues. In vitro binding assay showed that the cloned fragments could be co-complexed with nuclear matrix proteins to form insoluble complex easily removed by centrifugation.