DNA polymerase ε harmonizes topological states and R-loops formation to maintain genome integrity in Arabidopsis.

Genome topology is tied to R-loop formation and genome stability. However, the regulatory mechanism remains to be elucidated. By establishing a system to sense the connections between R-loops and genome topology states, we show that inhibiting DNA topoisomerase 1 (TOP1i) triggers the global increase of R-loops (called topoR-loops) and DNA damages, which are exacerbated in the DNA damage repair-compromised mutant atm.

Scaled consensus for second-order multi-agent systems subject to communication noise with stochastic approximation-type protocols.

This work is dedicated to the leaderless/leader-following stochastic scaled consensus issue of second-order stochastic multi-agent systems (SMASs) in a noisy environment. Scaled consensus represents that the ratios among agents asymptotically tend to designated constants rather than the common convergence value. To lessen the influence of communication noise, some stochastic approximation protocols with time-varying gain are designed for our underlying system, where the time-varying gain remove the restriction of nonnegative value.

Aberrant accumulation of Kras-dependent pervasive transcripts during tumor progression renders cancer cells dependent on PAF1 expression.

KRAS is the most commonly mutated oncogene in human cancer, and mutant KRAS is responsible for over 90% of pancreatic ductal adenocarcinoma (PDAC), the most lethal cancer. Here, we show that RNA polymerase II-associated factor 1 complex (PAF1C) is specifically required for survival of PDAC but not normal adult pancreatic cells. We show that PAF1C maintains cancer cell genomic stability by restraining overaccumulation of enhancer RNAs (eRNAs) and promoter upstream transcripts (PROMPTs) driven by mutant Kras.

Anatomically distinct fibroblast subsets determine skin autoimmune patterns.

The skin serves as a physical barrier and an immunological interface that protects the body from the external environment. Aberrant activation of immune cells can induce common skin autoimmune diseases such as vitiligo, which are often characterized by bilateral symmetric lesions in certain anatomic regions of the body. Understanding what orchestrates the activities of cutaneous immune cells at an organ level is necessary for the treatment of autoimmune diseases.

Hair shaft miniaturization causes stem cell depletion through mechanosensory signals mediated by a Piezo1-calcium-TNF-α axis.

In aging, androgenic alopecia, and genetic hypotrichosis disorders, hair shaft miniaturization is often associated with hair follicle stem cell (HFSC) loss. However, the mechanism causing this stem cell depletion in vivo remains elusive. Here we show that hair shaft loss or a reduction in diameter shrinks the physical niche size, which results in mechanical compression of HFSCs and their apoptotic loss.

Crosstalk Analysis and Performance Evaluation for Torus-Based Optical Networks-on-Chip Using WDM.

Insertion loss and crosstalk noise will influence network performance severely, especially in optical networks-on-chip (ONoCs) when wavelength division multiplexing (WDM) technology is employed. In this paper, an insertion loss and crosstalk analysis model for WDM-based torus ONoCs is proposed to evaluate the network performance. To demonstrate the feasibility of the proposed methods, numerical simulations of the WDM-based torus ONoCs with optimized crossbar and crux optical routers are presented, and the worst-case link and network scalability are also revealed.

Serum Cholesterol-Lowering Activity of β-Sitosterol Laurate Is Attributed to the Reduction of Both Cholesterol Absorption and Bile Acids Reabsorption in Hamsters.

The research was performed to delineate how β-sitosterol laurate (β-SLE) consumption influenced serum and hepatic lipids. The results showed that 220 mg/5 mL oil/kg body weight of β-SLE robustly reduced serum total triglyceride and cholesterol levels and the epididymal adipocyte size, and efficiently protected hepatic polyunsaturated fatty acids against lipid peroxidation through superoxide dismutase and glutathione transferase activity enhancement and malondialdehyde level reduction. Based on the changes of fecal cholesterol contents, fecal and hepatic bile acid (BAs) levels, and related protein expression, it was concluded that the mechanisms for lowering serum cholesterol by β-SLE involved (i) the enhanced excretion of fecal cholesterol via down-regulation of intestinal Niemann-Pick C1-like 1 protein; (ii) the increased conversion from cholesterol to primary BAs via up-regulation of cholesterol-7α-hydroxylase and sterol 27-hydroxylase, which was induced by the reduced BAs reabsorption through up-regulating ileal apical sodium-dependent bile acid transporter and ileal bile acid-binding protein.

The complete chloroplast genome of Papaver setigerum and comparative analyses in Papaveraceae.

Papaver setigerum is an annual herb that is closely related to the opium poppy, P. somniferum. Genetic resources for P.

Consumption of Interesterified Medium- and Long-Chain Triacylglycerols Improves Lipid Metabolism and Reduces Inflammation in High-Fat Diet-Induced Obese Rats.

Medium- and long-chain triacylglycerols (MLCTs) were synthesized from rapeseed oil (RO), one kind of commonly used edible long-chain triacylglycerols (TGs), and then delivered to high-fat diet (HFD)-induced obese rats. Compared with RO, MLCT consumption exhibited more potent effects on reducing body and tissue weight gains, plasma TG, and total cholesterol (TC) levels and on improving hepatic TG, TC, fatty acid synthase, acetyl-CoA carboxylase, and lipoprteinlipase contents. Meanwhile, lower amounts of tumor necrosis factor-α (TNF-α), monocyte chemoattractant protein-1, and endotoxin in plasma, lower levels of interleukin-6 and TNF-α, and higher levels of interleukin-10 in both livers and white adipose tissues were detected in MLCT-fed rats.

Plastome Evolution in Saxifragaceae and Multiple Plastid Capture Events Involving and .

Saxifragaceae, a family of over 600 species and approximately 30 genera of herbaceous perennials, is well-known for intergeneric hybridization. Of the main lineages in this family, the group represents a valuable model for the analysis of plastid capture and its impact on phylogeny reconstruction. In this study, we investigated plastome evolution across the family, reconstructed the phylogeny of the group and examined putative plastid capture between and .

Complete plastome sequence of and phylogenetic implications in Oleaceae.

(Oleaceae) is mainly distributed in the southwest of China and also planted in arboretums as an ornamental plant. In the present study, the plastome of was reconstructed using genome skimming sequencing, and the phylogeny analysis was inferred based on whole plastome data. The plastome of is 155,259 bp in length, comprising two copies of inverted regions (IR, 25,680 bp) separated by the large single copy (LSC, 86,534 bp) and small single copy (SSC, 17,365 bp) regions.

Complete chloroplast genome sequence and phylogenetic analysis of .

is commonly known as treacle-mustard or wormseed wallflower with value for reducing high temperature and inducing diuresis. Here, we characterized the complete chloroplast (cp) genome of using genome skimming sequencing. The circular complete cp genome is 154,611 bp in length, containing a large single-copy (LSC) region of 83,809 bp, two copies of IR (26,475 bp each) regions, and a small single-copy (SSC) region of 17,852 bp.