SAM-dPCR: Accurate and Generalist Nuclei Acid Quantification Leveraging the Zero-Shot Segment Anything Model.

Digital PCR (dPCR) has transformed nucleic acid diagnostics by enabling the absolute quantification of rare mutations and target sequences. However, traditional dPCR detection methods, such as those involving flow cytometry and fluorescence imaging, may face challenges due to high costs, complexity, limited accuracy, and slow processing speeds. In this study, SAM-dPCR is introduced, a training-free open-source bioanalysis paradigm that offers swift and precise absolute quantification of biological samples.

A comprehensive review of TGA transcription factors in plant growth, stress responses, and beyond.

TGA transcription factors (TFs), belonging to the D clade of the basic region leucine zipper (bZIP) family, exhibit a specific ability to recognize and bind to regulatory elements with TGACG as the core recognition sequence, enabling the regulation of target gene expression and participation in various biological regulatory processes. In plant growth and development, TGA TFs influence organ traits and phenotypes, including initial root length and flowering time. They also play a vital role in responding to abiotic stresses like salt, drought, and cadmium exposure.

Highly dispersive Pt-Pd nanoparticles on graphene oxide sheathed carbon fiber microelectrodes for electrochemical detection of HO released from living cells.

We report a facile strategy for the synthesis of surfactant-free, small and highly dispersive Pt-Pd nanoparticles on graphene oxide (Pt-Pd NPs/GO) by an electroless deposition method, which is sheathed on carbon fiber microelectrodes (CFMs) as an electrochemical sensing platform for highly sensitive and selective detection of hydrogen peroxide (HO) released from the living cells. GO serves as the reducing agent and stabilizer for electroless deposition of Pd NPs on the surface of GO owing to its low work function (4.38 eV) and highly conjugated electronic structure.