Sensors (Basel)
January 2025
The objective of this study is to accurately, expeditiously, and efficiently identify the wear state of milling cutters. To this end, a state identification method is proposed that combines continuous wavelet transform and an improved MobileViT lightweight network. The methodology involves the transformation of the cutting force signal during the milling cutter cutting process into a time-frequency image by continuous wavelet transform.
View Article and Find Full Text PDFAs biological research has synthesized genomics, proteomics, metabolomics, and transcriptomics into systems biology, a new multiomics approach to biological research has emerged. Today, multiomics studies are challenging and expensive. An experimental platform that could unify the multiple omics approaches to measurement could increase access to multiomics data by enabling more individual labs to successfully attempt multiomics studies.
View Article and Find Full Text PDFThe prevailing philosophy in biological testing has been to focus on simple tests with easy to interpret information such as ELISA or lateral flow assays. At the same time, there has been a decades long understanding in device physics and nanotechnology that electrical approaches have the potential to drastically improve the quality, speed, and cost of biological testing provided that computational resources are available to analyze the resulting complex data. This concept can be conceived of as "the internet of biology" in the same way miniaturized electronic sensors have enabled "the internet of things.
View Article and Find Full Text PDFImprovement upon, and expansion of, diagnostic tools for clinical infections have been increasing in recent years. The simplicity and rapidity of techniques are imperative for their adoption and widespread usage at point-of-care. The fabrication and evaluation of such a device is reported in this work.
View Article and Find Full Text PDFMethods Mol Biol
March 2019
DNA nanostructures and hybrid DNA-protein materials are attractive solutions to many applications in biotechnology and material science because of their controllable molecule-level features. Critical to a complete description and characterization of these technologies is the quantification of binding affinity between DNA nanostructures and small molecules relevant to the application at hand. This protocols chapter described a series of experimental and in silico analyses that can be used to described and quantify ligand binding interactions between DNA nanostructures (DNA DX tiles), short double stranded DNA fragments, and arbitrary small molecules.
View Article and Find Full Text PDFUnderstanding the chemical and physical interactions at the interface of protein surfaces and inorganic crystals has important implications in the advancement of immobilized enzyme catalysis. Recently, enzyme-inorganic hybrid complexes have been demonstrated as effective materials for enzyme immobilization. The precipitation of phosphate nanocrystals in the presence of enzymes creates enzyme-Cu(PO)·3HO particles with high surface-to-volume ratios, enhanced activity, and increased stability.
View Article and Find Full Text PDFUnderstanding the molecular interactions between small molecules and double-stranded DNA has important implications on the design and development of DNA and DNA-protein nanomaterials. Such materials can be assembled into a vast array of 1-, 2-, and 3D structures that contain a range of chemical and physical features where small molecules can bind via intercalation, groove binding, and electrostatics. In this work, we use a series of simulation-guided binding assays and spectroscopy techniques to investigate the binding of selected organophosphtates, methyl parathion, paraoxon, their common enzyme hydrolysis product p-nitrophenol, and double-stranded DNA fragments and DNA DX tiles, a basic building block of DNA-based materials.
View Article and Find Full Text PDFUnderstanding and controlling the molecular interactions between enzyme substrates and DNA nanostructures has important implications in the advancement of enzyme-DNA technologies as solutions in biocatalysis. Such hybrid nanostructures can be used to create enzyme systems with enhanced catalysis by controlling the local chemical and physical environments and the spatial organization of enzymes. Here we have used molecular simulations with corresponding experiments to describe a mechanism of enhanced catalysis due to locally increased substrate concentrations.
View Article and Find Full Text PDFWe have designed, fabricated, calibrated and tested actuators for shear characterization to assess microscale shear properties of soft substrates. Here we demonstrate characterization of dry silicone and hydrated polyethelyne glycol. Microscale tools, including atomic force microscopes and nanoindenters, often have limited functionality in hydrated environments.
View Article and Find Full Text PDFSmall RNA (19-23 nucleotides) molecules play an important role in gene regulation, embryonic differentiation, hematopoiesis, and a variety of cancers. Here, we present an ultrasensitive, extremely specific, label-free, and rapid electronic detection of microRNAs (miRNAs) using a carbon nanotubes field-effect transistor functionalized with the Carnation Italian ringspot virus p19 protein biosensor. miRNA-122a was chosen as the target, which was first hybridized to a probe molecule.
View Article and Find Full Text PDFAppl Biochem Biotechnol
July 2013
Nanostructures, such as nanowires, nanobelts, nanosprings, and nanotubes, are receiving growing interest as transducer elements of bio/chemical sensors as they provide high sensitivity, multiplexing, small size, and portability. Single-walled carbon nanotubes (SWNTs) are one such class of nanostructure materials that exhibit superior sensing behavior due to its large-surface carbon atoms that are highly responsive to surface adsorption events. Further, their compatibility with modern microfabrication technologies and facile functionalization with molecular recognition elements make them promising candidates for bio/chemical sensors applications.
View Article and Find Full Text PDFSolution processed thin films of an amphiphilic tris(phthalocyaninato) rare earth triple-decker complex, Eu(2)[Pc(15C5)(4)](2)[Pc(OC(10)H(21))(8)], have been prepared from three different methods: self-assembly (SA) annealed in solvent vapor, quasi-Langmuir-Shäfer (QLS) and drop casting methods. In particular, we successfully developed a simple QLS process for fabricating ordered multilayers with a good thickness control. The films prepared from three different methods were characterized by a wide range of methods including electronic absorption spectra, IR, X-ray diffraction, atomic force microscopy (AFM), and current-voltage (I-V) measurements.
View Article and Find Full Text PDFA new pathway by means of transetherification has been developed to synthesize novel unsymmetrical 2,3,9,10,16,17,23-heptakis(alkoxyl)-24-mono(dimethylaminoalkoxyl)phthalocyanine compounds. Cyclic tetramerization of 4,5-di(alkoxyl)phthalonitrile in refluxing dimethylamino-alcohol with high boiling point such as dimethylaminoethanol (DMAE) and 1-dimethylamino-2-propanol in the presence of lithium and pyrazino[2,3-f][1,10]phenanthroline-2,3-dicarbonitrile followed by treatment with acetic acid led to the isolation of a series of unsymmetrical metal free 2,3,9,10,16,17,23-heptakis(alkoxyl)-24-mono(dimethylaminoalkoxyl)phthalocyanine compounds H(2){Pc(OR)(7)[OR'N(CH(3))(2)]} [R = C(4)H(9), C(5)H(11), C(8)H(17) and R' = C(2)H(4); R = C(4)H(9) and R' = CH(CH(3))CH(2)] (1-4). Metal insertion into unsymmetrical metal free phthalocyanines (Pc's) using Cu(OAc)(2)·H(2)O in dimethylformamide (DMF) at 140 °C easily afforded corresponding unsymmetrical phthalocyaninato copper complexes Cu{Pc(OR)(7)[OR'N(CH(3))(2)]} (5-8) in good yields.
View Article and Find Full Text PDFHomoleptic octa-alpha-substituted bis(phthalocyaninato) rare earth double-deckers HM(III)[Pc(alpha-OC(4)H(9))(8)](2) [M = Eu (1), Y (2); Pc(alpha-OC(4)H(9))(8) = 1,4,8,11,15,18,22,25-octa(butyloxyl)phthalocyanine] have been prepared by treating the metal-free phthalocyanine H(2)Pc(alpha-OC(4)H(9))(8) with the corresponding M(acac)(3).nH(2)O (acac = acetylacetonate) in the presence of organic base 1,8-diazabicyclo[5.4.
View Article and Find Full Text PDFTo investigate the effects of metal-ligand coordination on the molecular structure, internal structure, dimensions, and morphology of self-assembled nanostructures, two nonperipherally octa(alkoxyl)-substituted phthalocyanine compounds with good crystallinity, namely, metal-free 1,4,8,11,15,18,22,25-octa(butyloxy)phthalocyanine H(2)Pc(alpha-OC(4)H(9))(8) (1) and its lead complex Pb[Pc(alpha-OC(4)H(9))(8)] (2), were synthesized. Single-crystal X-ray diffraction analysis revealed the distorted molecular structure of metal-free phthalocyanine with a saddle conformation. In the crystal of 2, two monomeric molecules are linked by coordination of the Pb atom of one molecule with an aza-nitrogen atom and its two neighboring oxygen atoms from the butyloxy substituents of another molecule, thereby forming a Pb-connected pseudo-double-decker supramolecular structure with a domed conformation for the phthalocyanine ligand.
View Article and Find Full Text PDFTwo amphiphilic heteroleptic tris(phthalocyaninato) europium complexes with hydrophilic crown ether heads and hydrophobic octyloxy tails [Pc(mCn)(4)]Eu[Pc(mCn)(4)]Eu[Pc(OC(8)H(17))(8)] [m = 12, n = 4, H(2)Pc(12C4)(4) = 2,3,9,10,16,17,23,24-tetrakis(12-crown-4)phthalocyanine; m = 18, n = 6, H(2)Pc(18C6)(4) = 2,3,9,10,16,17,23,24-tetrakis(18-crown-6)phthalocyanine; H(2)Pc(OC(8)H(17))(8) = 2,3,9,10,16,17,23,24-octakis(octyloxy)phthalocyanine] (1, 2) were designed and prepared from the reaction between homoleptic bis(phthalocyaninato) europium compound [Pc(mCn)(4)]Eu[Pc(mCn)(4)] (m = 12, n = 4; m = 18, n = 6) and metal-free H(2)Pc(OC(8)H(17))(8) in the presence of Eu(acac)(3).H(2)O (Hacac = acetylacetone) in boiling 1,2,4-trichlorobenzene. These novel sandwich triple-decker complexes were characterized by a wide range of spectroscopic methods and electrochemically studied.
View Article and Find Full Text PDFNovel metal-free 5,15-di[4-(5-acetylsulfanylpentyloxy)phenyl]porphyrin H2[DP(CH3COSC5H10O)2P] (1) and its zinc congener Zn[DP(CH3COSC5H10O)2P] (2) were designed and synthesized. Single-crystal X-ray diffraction (XRD) analysis confirmed the tetrapyrrole nature of these two compounds, revealing the existence of metal-ligand coordination bond between the carbonyl oxygen in the aryloxy side chain of meso-attached phenyl group in the porphyrin molecule with the zinc center of neighboring porphyrin molecule in the crystal structure of 2. This intermolecular Zn-O coordination bond induces the formation of a supramolecular chain structure in which the porphyrinato zinc moieties are arranged in a "head-to-tail" mode (J-aggregate), which is in contrast to a "face-to-face" stacking mode (H-aggregate) in the supramolecular structure formed depending on the C-H.
View Article and Find Full Text PDFA series of four amphiphilic heteroleptic tris(phthalocyaninato) europium complexes with different lengths of hydrophobic alkoxy substituents on one outer phthalocyanine ligand [Pc(15C5)4]Eu[Pc(15C5)4]Eu[Pc(OCnH(2n+1))8] (n = 4, 6, 10,12) (1, 2, 4, and 5) was designed and prepared. Their film forming and organic field effect transistor properties have been systematically studied in comparison with analogous [Pc(15C5)4]Eu[Pc(15C5)4]Eu[Pc(OC8H17)8] (3). Experimental results showed that all these typical amphiphilic sandwich triple-decker molecules have been fabricated into highly ordered films by the Langmuir-Blodgett (LB) technique, which displays carrier mobility in the direction parallel to the aromatic phthalocyanine rings in the range of 0.
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