Leonurine alleviates acetaminophen-induced acute liver injury by regulating the PI3K/AKT signaling pathway in mice.

Leonurine (Leo) is a natural alkaloid isolated from the herb Leonurus japonicus Houtt. (Leonuri) that has been shown to inhibit oxidative stress and inflammation. However, the role and mechanism of Leo in acetaminophen (APAP)-induced acute liver injury (ALI) remain unknown.

Mutations in the gene encoding Aminodeoxychorismate Synthase confer auxotrophic phenotypes.

To facilitate genetic mapping of developmental mutants of , we produced a genetic marker that combines recessive auxotrophy with dominant positive selection. We first identified the gene affected by the auxotrophic mutation and then replaced it with a cassette that confers antibiotic resistance. This strain may be used to produce bi-parental somatic hybrids with nearly any other strain.