An Enhanced Direct Position Determination of Mixed Circular and Non-Circular Sources Using Moving Virtual Interpolation Array.

In this study, a moving single-station direct position determination (DPD) algorithm based on virtual interpolated arrays is proposed. Existing moving single-station algorithms face challenges such as the incomplete utilization of sparse array apertures and insufficient consideration of mixed circular and non-circular signals. To address these issues, we propose an enhanced gridless DPD algorithm, suitable for multiple mixed circular and non-circular sources.

[Prognostic Value of DTA Mutations in Patients with Newly Diagnosed Acute Myeloid Leukemia].

Objective: To investigate the prognostic significance of DTA ( ) gene mutations in patients with non-M3 acute myeloid leukemia (AML).

Methods: The clinical data of 180 newly diagnosed AML patients hospitalized in the First People's Hospital of Changzhou from January 2018 to April 2022 were retrospectively analyzed. Next-generation sequencing technology was used to detect 150 gene mutations in the patients, and log-rank tests and Cox regression models were used to analyze the prognostic factors.

A ratiometric fluorescent probe based on UiO-66-TCPP for selective and visual detection of quercetin in food.

Quercetin (QCT) is a flavonoid with significant health benefits, necessitating sensitive detection methods for food safety and quality control. This study presents a novel UiO-66-TCPP ratiometric fluorescent probe for the quantitative and visual detection of QCT. Under optimal conditions, the fluorescence intensity of UiO-66-TCPP decreased linearly with increasing QCT concentration, with a detection limit of 26 nM.

A Duplex PCR Assay for Rapid Detection of and in Large Yellow Croaker Fish.

Both and cause an increasing number of diseases in fish, resulting in great economic losses in aquaculture. In addition, the disease infected with or exhibited the similar clinical symptoms in aquatic animals. However, there is no effective means for the simultaneous detection of co-infection and discrimination them for these two pathogens.

Comparing the Volatile and Soluble Profiles of Fermented and Integrated Chinese Bayberry Wine with HS-SPME GC-MS and UHPLC Q-TOF.

To evaluate the flavor characteristics of Chinese bayberry alcoholic beverages, fermented bayberry wine (FBW) and integrated bayberry wine (IBW) were investigated for their volatile and soluble profiles using HS-SPME GC-MS and UHPLC Q-TOF and were analyzed with multidimensional statistical analysis, including PCA and OPLS-DA. The volatile compounds 1-pentanol, β-caryophyllene and isopentanol were only detected in IBW. β-caryophyllene, the key flavor component of bayberry, was found to be the most abundant volatile compound in IBW (25.

Immunogenic characteristics of the outer membrane phosphoporin as a vaccine candidate against Klebsiella pneumoniae.

In recent years, Klebsiella pneumoniae (KP) has caused disease outbreaks in different animals, resulting in serious economic losses and biosafety concerns. Considering the broad antibiotic resistance of KP, vaccines are the most effective tools against infection. However, there is still no KP vaccine available in the veterinary field.

Metabolic changes of Issatchenkia orientalis under acetic acid stress by transcriptome profile using RNA-sequencing.

Issatchenkia orientalis (I. orientalis) is tolerant to various environmental stresses especially acetic acid stress in wine making. However, limited literature is available on the transcriptome profile of I.

A Method of Constructing Measurement Matrix for Compressed Sensing by Chebyshev Chaotic Sequence.

In this paper, the problem of constructing the measurement matrix in compressed sensing is addressed. In compressed sensing, constructing a measurement matrix of good performance and easy hardware implementation is of interest. It has been recently shown that the measurement matrices constructed by Logistic or Tent chaotic sequences satisfy the restricted isometric property (RIP) with a certain probability and are easy to be implemented in the physical electric circuit.

Identification of a novel cell-penetrating peptide derived from the capsid protein of chicken anemia virus and its application in gene delivery.

Cell membranes are a great obstacle for entrance of gene therapeutic agents. Cell-penetrating peptides (CPPs) have been proven as a promising gene delivery tool. However, the early TAT peptide derived from the HIV transcription activator protein has been proven that the sequence contains Furin protease cleaved motifs which limited the TAT application in delivery of exogenous active molecules.

Integrated transcriptomic and proteomic analysis of the acetic acid stress in Issatchenkia orientalis.

Issatchenkia orientalis known as a multi-tolerant non-Saccharomyces yeast, which tolerant environmental stresses, exhibits potential in wine making and bioethanol production. It is essential for the growth of I. orientalis to tolerant acetic acid in the mixed cultures with Saccharomyces cerevisiae.

Integrated transcriptomic and proteomic analysis of the ethanol stress response in Saccharomyces cerevisiae Sc131.

The molecular mechanism of Saccharomyces cerevisiae tolerant to ethanol stress remains to be further elucidated. In this study, a comprehensive analysis based on RNA-seq and iTRAQ LC-MS/MS was used to investigate the global mechanism of S. cerevisiae strain Sc131 in response to ethanol stress at transcriptomic and proteomic levels.

Transcriptome profiling of Issatchenkia orientalis under ethanol stress.

Issatchenkia orientalis, a non-Saccharomyces yeast that can resist a wide variety of environmental stresses, has potential use in winemaking and bioethanol production. Little is known about gene expression or the physiology of I. orientalis under ethanol stress.

Investigating the underlying mechanism of Saccharomyces cerevisiae in response to ethanol stress employing RNA-seq analysis.

Saccharomyces cerevisiae has been widely used for wine fermentation and bio-fuels production. A S. cerevisiae strain Sc131 isolated from tropical fruit shows good fermentation properties and ethanol tolerance, exhibiting significant potential in Chinese bayberry wine fermentation.

The modulatory effect of (-)-epigallocatechin 3-O-(3-O-methyl) gallate (EGCG3″Me) on intestinal microbiota of high fat diet-induced obesity mice model.

(-)-Epigallocatechin 3-O-(3-O-methyl) gallate (EGCG3″Me) has exhibited multiple beneficial effects on the prevention of obesity in oolong tea. However, its absorption is relatively low, and the potential to be fully utilized is not completely elucidated. Therefore, with human flora-associated (HFA) mice model, the effect of EGCG3″Me on high fat diet-induced obesity was investigated by high-throughput sequencing.

Metabolic Engineering of Wheat Provitamin A by Simultaneously Overexpressing CrtB and Silencing Carotenoid Hydroxylase (TaHYD).

Increasing the provitamin A content in staple crops via carotenoid metabolic engineering is one way to address vitamin A deficiency. In this work a combination of methods was applied to specifically increase β-carotene content in wheat by metabolic engineering. Endosperm-specific silencing of the carotenoid hydroxylase gene (TaHYD) increased β-carotene content 10.

Enrichment of provitamin A content in wheat (Triticum aestivum L.) by introduction of the bacterial carotenoid biosynthetic genes CrtB and CrtI.

Carotenoid content is a primary determinant of wheat nutritional value and affects its end-use quality. Wheat grains contain very low carotenoid levels and trace amounts of provitamin A content. In order to enrich the carotenoid content in wheat grains, the bacterial phytoene synthase gene (CrtB) and carotene desaturase gene (CrtI) were transformed into the common wheat cultivar Bobwhite.

Expression, purification and antimicrobial activity of puroindoline A protein and its mutants.

Wheat puroindoline proteins, PINA and PINB, play key roles in determining wheat grain hardness as well as in defending the plant against pathogens. PINA has much greater membrane-binding property and antimicrobial activity because it contains more tryptophan residues in the unique tryptophan-rich domain (TRD). In order to obtain proteins with higher antimicrobial activity, mutants of PINA containing two or three copies of TRD, designated ABBC and ABBBC, respectively, were constructed and expressed in E.

Expression of the 1Ax1 transgene in an elite Chinese wheat variety and its effect on functional properties.

Background: The transfer of transgenes from 'model' wheat genotypes into elite wheat cultivars using conventional plant breeding is an alternative strategy for improving the dough quality of wheat. Thus a cross was made between a popular Chinese elite wheat cultivar of the Yangzi River down-central area that expresses high-molecular-weight glutenin subunit (HMW-GS): 1Bx7 + 1By8 plus 1Dx2 + 1Dy12, and a model transgenic wheat line B102-1-2 which over-expresses HMW-GS 1Ax1 in an L88-31 genetic background that includes HMW-GS 1Bx17 + By18.

Results: F(1) to F(6) generations of crosses between B102-1-2 (paternal) and Emai (maternal) were analysed for their HMW-GS compositions, allowing the selection of pure F(6) lines over-expressing HMS-GS 1Ax1 in the presence of the endogenous HMW-GS: 1Bx7 + 1By8 with 1Dx2 + 1Dy12, and 1Bx17 + 1By18 with 1Dx2 + 1Dy12.