A sandwich-type electrochemiluminescence biosensor based on Ni(HAB)/Au@ZnNiAl-LDH/Ru@MIL-53(Al)-NH for ultra-sensitive detection of microRNA-155.

A novel electrochemiluminescence (ECL) biosensor was developed for the ultrasensitive detection of miRNA-155, based on the synergistic combination of multifunctional nanomaterials. The biosensor employed a conductive metal-organic framework (MOF), Ni(HAB) (HAB = hexaaminobenzene), as the substrate material. The unique π-electron conjugated structure of Ni(HAB) endowed the biosensor with excellent electron transport properties, significantly enhancing its sensitivity.

Bright NIR-II emissive cyanine dye-loaded lipoprotein-mimicking nanoparticles for fluorescence imaging-guided and targeted NIR-II photothermal therapy of subcutaneous glioblastoma.

Cyanine dye-containing nanoparticles have widely been used in "all-in-one" NIR fluorescence imaging (FI)-guided photothermal therapy (PTT) because of their intrinsically large extinction coefficient and available physical and chemical modulation methods to tune absorption and emission wavelengths. The combination of good brightness and excellent tumor-targeting capacity is the key to realize efficient NIR-II FI-guided PTT. In this study, by covalently decorating NIR-II absorptive cyanine dyes with bulky AIE motify, we demonstrate how steric hindrance suppresses π-π stacking-induced fluorescence quenching and contributes to the good brightness of NIR-II FI of subcutaneous glioblastoma.

Phlomoides rotata adapts to low-nitrogen environments by promoting root growth and increasing root organic acid exudate.

Nitrogen (N) is one of the three major elements required for plant growth and development. It is of great significance to study the effects of different nitrogen application levels on the growth and root exudates of Phlomoides rotata, and can provide a theoretical basis for its scientific application of fertilizer to increase production. In this study, Phlomoides rotata were grown under different nitrogen conditions for two months.

[Analysis of genetic association between exon polymorphisms of and and risky drinking behavior of alcoholic liver disease].

To analyze the correlation of exon rs1126671 and exon rs971074 polymorphisms with risky drinking behaviors and alcoholic liver disease. The patients with alcoholic liver disease diagnosed in the Gastroenterology Department of the People's Hospital of Hechi from November 2021 to June 2022, including 52 cases of alcoholic liver disease with positive risky drinking behaviors, 103 cases of non-alcoholic liver disease with positive risky drinking behaviors of the same gender and age, and 105 healthy subjects with no risky drinking behaviors as control groups were retrospectively analyzed. The serum total protein and albumin are detected by immunoturbidimetry and globulin is calculated by the difference method; the serum total bilirubin and direct bilirubin are detected by the nitrite oxidation method and indirect bilirubin is calculated by the difference method; alkaline phosphatase, aspartate aminotransferase, alanine aminotransferase and γ-glutamyl transferase are detected by the substrate method.

[Psychosocial and behavioral problems of children and adolescents across five cities in Northern China].

The objective of this research is to study the prevalence and risk factors of psychosocial and behavioral problems in children and adolescents of different ages and genders to provide a scientific foundation for more targeted psychological interventions and social support in the future. From April 21 to May 31, 2023, a cross-sectional survey was conducted using a stratified random sampling method in five cities (Beijing City, Changchun City, Baicheng City, Shenyang City, Hohhot City) across four provinces in Northern China (Beijing, Jilin, Liaoning, Inner Mongolia). The study was conducted using an online questionnaire among children and adolescents aged 6-16 years.

Absence of gut microbiota alleviates iron overload-induced colitis by modulating ferroptosis in mice.

Introduction: Iron overload disrupts gut microbiota and induces ferroptosis, contributing to colitis. However, whether gut microbiota directly drives iron overload-induced colitis and its underlying mechanism remain unclear.

Objectives: The study aimed to explore whether gut microbiota can directly regulate iron overload-induced colitis and its underling mechanism.

MDPAO1 Peptide from Human Milk Enhances Brown Adipose Tissue Thermogenesis and Mitigates Obesity.

The regulatory effect of breastfeeding on offspring metabolism has garnered significant attention as an effective strategy in combating childhood obesity. However, the underlying mechanism remains largely unknown. Through integrated analysis of multiple human milk peptide databases and functional screening, MDPAO1 (milk-derived peptide associated with obesity 1) was identified as having potential activity in promoting the expression of thermogenic genes.

A meta-analysis of dietary inhibitors for reducing methane emissions via modulating rumen microbiota in ruminants.

Background: Rumen methane emissions (RME) significantly contribute to global greenhouse gas emissions, underscoring the essentials to identify effective inhibitors for RME mitigation. Despite various inhibitors shown potential in reducing RME by modulating rumen microbes, their impacts include considerable variations and inconsistency.

Objective: We aimed to quantitively assess the impacts of various methane inhibitors on RME, rumen microbial abundance and fermentation in ruminants.

Halcinonide activates smoothened to ameliorate ischemic stroke injury.

Objectives: The Shh pathway may shed new light on developing new cell death inhibitors for the therapy of ischemic stroke. We aimed to examine whether the Shh co-reporter SMO or its agonist halcinonide can upregulate Bcl-2 to suppress neuronal cell death, ultimately improving behavioral deficits and reducing cerebral infarction in an ischemic stroke model.

Methods: Halcinonide or genetic manipulation of SMO was conducted in PC12 cells to examine their impacts on oxidative or OGD/R stress, and the chemical, along with AAV-SMO or AAV-EGFP were tested in MCAO rats to investigate their potential protective effects against neuronal damages due to cerebral I/R injury.

The RNA-binding protein CMSS1 promotes the progression of non-small cell lung cancer by regulating the telomerase protein subunit hTERT.

Aims: High telomerase activity has been detected in over 85 % of tumors, with the activation of hTERT being the most crucial mechanism for re-establishing telomerase activity. Activation of hTERT maintains telomere length in cells, enabling cancer cells to proliferate indefinitely. Nevertheless, the specific mechanism of telomerase activation in non-small cell lung cancer (NSCLC) remains unclear, and post-transcriptional regulation of hTERT could be a potential activation mechanism.

Regulating tumor cells to awaken T cell antitumor function and enhance melanoma immunotherapy.

Tumor cells transmit various immunosuppressive signals and induce a dysfunctional state in T cells, which essentially leads to immune escape and tumor progression. However, developing effective strategies to counteract the domestication of T cells by tumor cells remains a challenge. Here, we prepared pH-responsive lipid nanoparticles (NL/PLDs) co-loaded with PCSK9 shRNA, lonidamine (LND), and low-dose doxorubicin (DOX).

X-ray-activated nanoscintillators integrated with tumor-associated neutrophils polarization for improved radiotherapy in metastatic colorectal cancer.

Radiotherapy, employing high-energy rays to precisely target and eradicate tumor cells, plays a pivotal role in the treatment of various malignancies. Despite its therapeutic potential, the effectiveness of radiotherapy is hindered by the tumor's inherent low radiosensitivity and the immunosuppressive microenvironment. Here we present an innovative approach that integrates peroxynitrite (ONOO)-mediated radiosensitization with the tumor-associated neutrophils (TANs) polarization for the reversal of immunosuppressive tumor microenvironment (TME), greatly amplifying the potency of radiotherapy.

Modeling of heteroaggregation driven buoyant microplastic settling: Interaction with multiple clay particles.

The ecological risk of microplastics (MPs) has received widespread attention, but understanding ecological risk starts with understanding environmental migration. Heteroaggregation is an important process that affects the vertical migration of MPs, and the mathematical model is a common tool used to project the migration behavior of MPs. However, the mathematical model based on the aggregation of MPs with one clay particle is not applicable to simulate the migration behavior of buoyant microplastic (BMP).

Cobalt nanoparticles decorated hollow N-doped carbon nanospindles enable high-performance lithium-oxygen batteries.

Despite the ultrahigh theoretical energy density and cost-effectiveness, aprotic lithium-oxygen (Li-O) batteries suffer from slow oxygen redox kinetics at cathodes and large voltage hysteresis. Here, we well-design ultrafine Co nanoparticles supported by N-doped mesoporous hollow carbon nanospindles (Co@HCNs) to serve as efficient electrocatalysts for Li-O battery. Benefiting from strong metal-support interactions, the obtained Co@HCNs manifest high affinity for the LiO intermediate, promoting formation of ultrathin nanosheet-like LiO with low-impedance contact interface on the Co@HCNs cathode surface, which facilitates the reversible decomposition upon charging.

Oxidative stress-augmented Cu-doped hollow mesoporous carbon nanozyme for photothermal/photodynamic synergistic therapy.

Photodynamic therapy (PDT) has witnessed remarkable progress in recent years owing to its specific properties. Given that the antioxidation system of tumor microenvironment (TME) adversely affects treatment outcomes, powerful TME modulators can significantly resolve the limitation of PDT. Herein, we developed a PEG-modified Cu-doped hollow mesoporous carbon nanozyme (CHC-PEG) and loaded insoluble photosensitizer IR780 into its pores and cavities to construct the multifunctional nano-system IR780/CHCP.

Recentering the humeral head does not necessarily improve glenohumeral abduction: A cadaveric biomechanical study.

Purpose: To explore if recentering the humeral head could improve shoulder abduction in shoulders with irreparable massive rotator cuff tears (IMRCTs).

Methods: Nine fresh-frozen cadaveric shoulders were used to simulate the intact condition and IMRCTs as controls. Four 'recenter' interventions were then sequentially applied: subacromial interposition (SI) using 4- and 8-mm spacers and superior capsule reconstruction (SCR) using two- and four-layer fascia lata grafts.

Hormones synthesized by the adrenal reticulum protect bone density in premenopausal women with Cushing syndrome.

Background: Differences in bone metabolism between patients with adrenal Cushing's syndrome (ACS) and Cushing's disease (CD) have been noted, but the impact of steroid hormones on bone metabolism remains underexplored. The purpose of this study is to explore the differences in bone metabolism between the two subtypes of Cushing's syndrome and the correlation between hormones synthesized by the adrenal reticulum and bone metabolism.

Method: This retrospective study included 75 premenopausal women, consisting of 33 patients with CD and 42 patients with ACS.

Integrated analysis of biochemical, transcriptomic, and metabolomic response mechanisms in Ussuri catfish (Pseudobagrus ussuriensis) under acute heat stress.

Fish metabolism, growth, development, and physiological conditions are highly sensitive to fluctuations in water temperature. The Ussuri catfish (Pseudobagrus ussuriensis) is an important native economic species in China. However, research on heat stress in P.

Biomimetic "Trojan Horse" Fibers Modulate Innate Immunity Cascades for Nerve Regeneration.

Neutrophil membrane vesicles (NMVs) have been successfully applied to control the inflammatory cascade after spinal cord injury (SCI) by acting as an inflammatory factor decoy to front-load the overall inflammation regulatory window; however, the mechanisms by which NMVs regulate macrophage phenotypic shifts as well as their outcomes have rarely been reported. In this study, we demonstrated the "efferocytosis-like" effect of NMVs endocytosed by macrophages, supplementing the TCA cycle intermediate metabolite α-KG by promoting glutamine metabolism, which in turn facilitates oxidative phosphorylation and inhibits the NF-κB signaling pathway to reprogram inflammatory macrophages to the pro-regenerative phenotype. Based on these findings, a "Trojan horse" composite fiber scaffold was constructed; this comprised a carboxylated poly-l-lactic acid shell encapsulated with NMVs and a core loaded with brain-derived neurotrophic factor to spatiotemporally modulate the inflammatory microenvironment by 39.

Risk Stratification and Adjuvant Chemotherapy for High-Risk Stage IA Lung Adenocarcinoma: The Unmet Needs.

Introduction: To identify high-risk patients for recurrence in resected stage IA lung adenocarcinoma and evaluate the impact of adjuvant chemotherapy (ACT) on their prognosis, as well as explore potential novel adjuvant therapies.

Methods: Consecutive stage IA patients with ≥ 5% solid or micropapillary subtypes were analyzed. A nomogram was developed using Cox proportional hazards regression to predict recurrence-free survival (RFS).

Exploration of the Fertility Decision-Making Experiences of Women of Reproductive Age With Cancer: A Qualitative Systematic Review.

Women of reproductive age with cancer face unique considerations in terms of fertility. The related decision-making process is complicated, and insufficient support can lead to decisional conflict. The aim of this qualitative systematic review was to identify and integrate qualitative evidence regarding the fertility decision-making process of women of reproductive age undergoing treatment for cancer.

Religion, spirituality, and DNA methylation in HPA-axis genes among Hispanic/Latino adults.

Aim: Investigate associations between religion and spirituality (R&S) and DNA methylation of four HPA-axis genes (i.e. 14 CpG sites) among 992 adults from the Hispanic Community Health Study/Study of Latinos cohorts.

Mechanism of starvation induced autophagy and apoptosis in the midgut of silkworm, Bombyx mori, based on calcium homeostasis.

Starvation can induce autophagy and apoptosis in intestinal cells. To elucidate the underlying mechanisms, we investigated autophagy and apoptosis in the midgut of the model insect, silkworm (Bombyx mori), focusing on calcium homeostasis. The results indicated that the body weight of silkworms decreased, along with damage to the morphology of their digestive tracts and midguts after starvation treatment.

African swine fever virus enhances viral replication by increasing intracellular reduced glutathione levels, which suppresses stress granule formation.

African swine fever virus (ASFV) is a DNA virus that has significantly impacted the global swine industry. Currently, there are no effective therapies or vaccines against ASFV. Stress granules (SGs), known for their antiviral properties, are not induced during ASFV infection, even though reactive oxygen species (ROS) are generated.