Evaluation of guide-free Cas9-induced genomic damage and transcriptome changes in pig embryos.

Cas9 protein without sgRNAs can induce genomic damage at the cellular level . However, whether the detrimental effects occur in embryos after Cas9 treatment remains unknown. Here, using pig embryos as subjects, we observed that Cas9 protein transcribed from injected Cas9 mRNA can persist until at least the blastocyst stage.

The Role of Graphene Oxide in the Exothermic Mechanism of Al/CuO Nanocomposites.

Metastable intermixed composites (MICs) have received increasing attention in the field of energy materials in recent years due to their high energy and good combustion performance. The exploration of ways of improving their potential release of heat is still underway. In this study, Al-CuO/graphene oxide (GO) nanocomposites were prepared using a combination of the self-assembly and in-suit synthesis methods.

Modeling the Heating Dynamics of a Semiconductor Bridge Initiator with Deep Neural Network.

A semiconductor bridge (SCB) is an ignition device that provides a safe and efficient method widely used in civilian and military fields. The heating process of an SCB under electrical stimulation has a wide range of applications owing to its unique energy release process. However, the temperature variation of an SCB is challenging to obtain, both experimentally because of the rapid reaction on a microscale and with simulation due to its high demand in nonlinear calculations.

A bibliometric analysis of college students' entrepreneurial intention from 2000 to 2020: Research trends and hotspots.

Based on 454 articles related to college students' entrepreneurial intention in the Web of Science Core Collection, this study explores the hotspots and trends of international research on college students' entrepreneurial intention by using a combination of coding and bibliometric analysis. The research hotspots are as follows: the theory of planned behavior is the main theoretical basis of these studies; entrepreneurship education is a more important predictor of college students' entrepreneurial intention, and this relationship is regulated by multiple variables; personal traits, several types of capital theories, social entrepreneurial intention, and quantitative research methods are also common. The research fronts include the following: systematic review of the field, continuous attention to the theory of planned behavior, and in-depth exploration of the differentiated influence of entrepreneurship education on entrepreneurial intention.

Exploring the Interfacial Reaction of Nano Al/CuO Energetic Films through Thermal Analysis and Ab Initio Molecular Dynamics Simulation.

The effect of the interface layer on energy release in nanoenergetic composite films is important and challenging for the utilization of energy. Nano Al/CuO composite films with different modulation periods were prepared by magnetron sputtering and tested by differential scanning calorimetry. With the increase in the modulation period of the nano Al/CuO energetic composite films, the interface layer contained in the energetic composite film decreased meaningfully, increasing the total heat release meaningfully.

Double knock-in pig models with elements of binary Tet-On and phiC31 integrase systems for controllable and switchable gene expression.

Inducible expression systems are indispensable for precise regulation and in-depth analysis of biological process. Binary Tet-On system has been widely employed to regulate transgenic expression by doxycycline. Previous pig models with tetracycline regulatory elements were generated through random integration.

AGBE: a dual deaminase-mediated base editor by fusing CGBE with ABE for creating a saturated mutant population with multiple editing patterns.

Establishing saturated mutagenesis in a specific gene through gene editing is an efficient approach for identifying the relationships between mutations and the corresponding phenotypes. CRISPR/Cas9-based sgRNA library screening often creates indel mutations with multiple nucleotides. Single base editors and dual deaminase-mediated base editors can achieve only one and two types of base substitutions, respectively.

Offline and Online Social Support and Short-Form Video Addiction Among Chinese Adolescents: The Mediating Role of Emotion Suppression and Relatedness Needs.

Short-form video applications have gained worldwide popularity in recent years. Although short-form video applications encourage communication over the Internet, their popularity encourages adolescents to overindulge in them. This study aimed to explore the effects of offline and online social support (SS) for short-form video addiction (SVA), and the mediating role of emotion regulation and basic psychological needs.

Observations on Detonation Growth of Lead Azide at Microscale.

Lead azide (LA) is a commonly used primary explosive, the detonation growth of which is difficult to study because it is so sensitive and usually has a small charge size in applications. We used photon Doppler velocimetry (PDV) and calibrated polyvinylidene fluoride (PVDF) gauges to reveal the detonation growth in LA, which was pressed in the confinements with controlled heights. The particle-velocity profiles, output pressure, unsteady detonation velocity, reaction time, and reaction-zone width were obtained and analyzed.

Transitory restless arms syndrome in a patient with antipsychotics and antidepressants: a case report.

Background: Restless arms syndrome (RAS) is characterized by uncomfortable aching or burning sensations in the arms. RAS is regarded as an upper limb variant of restless legs syndrome (RLS). The lack of specific diagnostic criteria makes it difficult to recognize the RAS.

Fear of Missing Out and Irrational Procrastination in the Mobile Social Media Environment: A Moderated Mediation Analysis.

This study explored the relationship between Fear of Missing Out (FoMO) and irrational procrastination in a mobile social media environment and its underlying mechanism: the mediating role of cognitive failure. The study was conducted with 817 college students using the FoMO Scale, Irrational Procrastination Scale, Cognitive Failures Questionnaire, and Self-Control Scale. The results showed that (a) FoMO positively predicted irrational procrastination in the mobile social media environment; (b) cognitive failure had a complete mediating effect on the relationship between FoMO and irrational procrastination; and (c) self-control had a moderating effect on the relationship between FoMO and cognitive failure.

Specific Antecedents of Entrepreneurial Intention Among Newly Returned Chinese International Students.

A growing group of Chinese students is returning to China following graduation, especially young returnees. This group is seen as one of the most innovative sectors of Chinese society. Based on the theory of planned behavior (TPB) and three kinds of capital theories, this study explores entrepreneurial intention (EI) and its influencing factors among Newly Returned Chinese International Students (NRCIS).

Photo-Responsive Fluorosurfactant Enabled by Plasmonic Nanoparticles for Light-Driven Droplet Manipulation.

The past decade has witnessed a significant development of droplet microfluidics for applications such as directed evolution and single-cell analysis. While the stability and manipulation of droplets are part of the prerequisites to further their applications, most of the currently available surfactants serve solely as stabilizers between the interfaces of water and oil. In this study, we present a novel type of photo-responsive fluorosurfactant based on fluorinated plasmonic nanoparticles (NPs).

CRISPR/Cas9-Mediated Gene Correction in Newborn Rabbits with Hereditary Tyrosinemia Type I.

Patients with hereditary tyrosinemia type I (HT1) present acute and irreversible liver and kidney damage during infancy. CRISPR-Cas9-mediated gene correction during infancy may provide a promising approach to treat patients with HT1. However, all previous studies were performed on adult HT1 rodent models, which cannot authentically recapitulate some symptoms of human patients.

ACBE, a new base editor for simultaneous C-to-T and A-to-G substitutions in mammalian systems.

Background: Many favorable traits of crops and livestock and human genetic diseases arise from multiple single nucleotide polymorphisms or multiple point mutations with heterogeneous base substitutions at the same locus. Current cytosine or adenine base editors can only accomplish C-to-T (G-to-A) or A-to-G (T-to-C) substitutions in the windows of target genomic sites of organisms; therefore, there is a need to develop base editors that can simultaneously achieve C-to-T and A-to-G substitutions at the targeting site.

Results: In this study, a novel fusion adenine and cytosine base editor (ACBE) was generated by fusing a heterodimer of TadA (ecTadA) and an activation-induced cytidine deaminase (AID) to the N- and C-terminals of Cas9 nickase (nCas9), respectively.

Engineered Pigs Carrying a Gain-of-Function NLRP3 Homozygous Mutation Can Survive to Adulthood and Accurately Recapitulate Human Systemic Spontaneous Inflammatory Responses.

The NLRP3 inflammasome is associated with a variety of human diseases, including cryopyrin-associated periodic syndrome (CAPS). CAPS is a dominantly inherited disease with missense mutations. Currently, most studies on the NLRP3-inflammasome have been performed with mice, but the activation patterns and the signaling pathways of the mouse NLRP3 inflammasome are not always identical with those in humans.

An energetic composite formed of wrinkled rGO sheets wrapped around copper azide nanowires with higher electrostatic safety as a green primary explosive.

A green primary explosive with high energy density and electrostatic safety was synthesized in this work. A precursor consisting of wrinkled reduced graphene oxide sheets wrapped around copper nanowires (CuNWs@rGO) was fabricated through a facile one-pot hydrothermal approach. The as-prepared precursor was deposited on a silicon wafer by electrophoretic deposition technology, which significantly reduced the safety risks of directly handling the powder sample in the azide reaction.

In Situ Synthesized MEMS Compatible Energetic Arrays Based on Energetic Coordination Polymer and Nano-Al with Tunable Properties.

Integrating energetic materials with a microelectromechanical system (MEMS) to achieve miniaturized integrated smart energetic microchips is promising. The potential applications include actuation in lab-on-a-chip devices, ignition in automobile airbags, propulsion and attitude control of micro-/nano-satellites, and miniaturized electro-explosive devices. In this work, a new type of MEMS-compatible energetic arrays was in situ realized on a copper substrate, which comprised a new energetic coordination polymer (ECP; CuCNO·HO) with tunable nanostructures and a nano-aluminum (nano-Al) covering layer.

Energetic Films Realized by Encapsulating Copper Azide in Silicon-based Carbon Nanotube Arrays with Higher Electrostatic Safety.

Since copper azide (Cu(N)) has high electrostatic sensitivity and is difficult to be practically applied, silicon-based Cu(N)@carbon nanotubes (CNTs) composite energetic films with higher electrostatic safety were fabricated, which can be compatible with micro-electro mechanical systems (MEMS). First, a silicon-based porous alumina film was prepared by a modified two-step anodic oxidation method. Next, CNTs were grown in pores of the silicon-based porous alumina film by chemical vapor deposition.