and Antigen Presentation and Diagnosis Development of Recombinant Overlapping Peptides Corresponding to ESAT-6/CFP-10.

Cellular immunity in () infection is important for the pathogenesis and final clearance of intracellular infection. In addition, it is valuable for the diagnosis of tuberculosis. In this pioneering work, we tested and antigen presentation and diagnostic application of a recombinant overlapping peptide-protein derived from two RD1 antigens ESAT-6 and CFP-10 (ROP-TB).

A fragment activity assay reveals the key residues of TBC1D15 GTPase-activating protein (GAP) in Chiloscyllium plagiosum.

Background: GTPase-activating proteins (GAPs) with a TBC (Tre-2/Bub2/Cdc16) domain architecture serve as negative regulators of Rab GTPases. The related crystal structure has been studied and reported by other members of our research group in 2017 (Chen et al. in Protein Sci 26(4):834-846, 2017).

Crystal structure of TBC1D15 GTPase-activating protein (GAP) domain and its activity on Rab GTPases.

TBC1D15 belongs to the TBC (Tre-2/Bub2/Cdc16) domain family and functions as a GTPase-activating protein (GAP) for Rab GTPases. So far, the structure of TBC1D15 or the TBC1D15·Rab complex has not been determined, thus, its catalytic mechanism on Rab GTPases is still unclear. In this study, we solved the crystal structures of the Shark and Sus TBC1D15 GAP domains, to 2.

Differential expression and miRNA regulation of the GSTP1 gene in the regenerating liver of Chiloscyllium plagiosum.

Liver regeneration is a complicated process, and understanding the regulatory mechanism will be helpful in the treatment of diseases associated with liver. In this study, the one-third liver resection model was established in Chiloscyllium plagiosum, and the whole transcriptome of the C. plagiosum was generated using the Illumina-Solexa sequencing platform.

Design, Synthesis and Biological Evaluation of Stilbene Derivatives as Novel Inhibitors of Protein Tyrosine Phosphatase 1B.

By imitating the scaffold of lithocholic acid (LCA), a natural steroidal compound displaying Protein Tyrosine Phosphatase 1B (PTP1B) inhibitory activity, a series of stilbene derivatives containing phenyl-substituted isoxazoles were designed and synthesized. The structures of the title compounds were confirmed by ¹H-NMR, C-NMR and HRMS. Activities of the title compounds were evaluated on PTP1B and the homologous enzyme TCPTP by using a colorimetric assay.

Detection of piRNAs in whitespotted bamboo shark liver.

Piwi-interacting RNAs (piRNAs) are 26 to 31-nt small non-coding RNAs that have been reported mostly in germ-line cells and cancer cells. However, the presence of piRNAs in the whitespotted bamboo shark liver has not yet been reported. In a previous study of microRNAs in shark liver, some piRNAs were detected from small RNAs sequenced by Solexa technology.

Studies on land application of sewage sludge and its limiting factors.

Field experiments were conducted to study the effect of sewage sludge application on the heavy metal content in soils and grasses. The sewage sludge was obtained from Northern Shenyang Wastewater Treatment Plant, China, and applied at 0, 15, 30, 60, 120 and 150tha(-1). Native grasses Zoysia japonica and Poa annua were chosen as experimental plants.

[Effects of land utilization of sewage sludge on grass and soils].

Effects of land disposal of sewage sludge on grass and soil environment were studied. The sewage sludge used was from Northern Shenyang Wastewater Treatment Plant. The results showed that contents of nutrient in the soil were increased after sewage sludge application, especially for organic matter.