Genomic gain/methylation modification/hsa-miR-132-3p increases RRS1 overexpression in liver hepatocellular carcinoma.

This study aimed to determine the upstream regulatory factors affecting ribosome biogenesis regulator 1 homolog (RRS1) expression and the development and prognosis of liver hepatocellular carcinoma (LIHC). The expression profiles of RRS1 were evaluated in pan-cancer tissues and liver tumor cell lines. The associations of RRS1 with pan-cancer survival, immune infiltrations, immune checkpoints, and drug sensitivity were identified.

[Oxidative stress preconditioning alleviates oxidative stress-induced damage of bone marrow mesenchymal stem cells].

Objective To investigate the effect of oxidative stress preconditioning on the oxidative stress-induced damage of bone marrow mesenchymal stem cells (BMSCs). Methods BMSCs were isolated and cultured by density gradient centrifugation combined with adherence method. The cells were divided into three groups: control group (control medium), oxidative damage group (treated with 1000 μmol/L HO for 24 hours), and preconditioning group (preincubated with 50 μmol/L HO for 8 hours before treatment with 1000 μmol/L HO for 24 hours).

PARK7 enhances antioxidative-stress processes of BMSCs via the ERK1/2 pathway.

Oxidative stresss in the microenvironment surrounding lesions induces apoptosis of transplanted bone-marrow-derived mesenchymal stem cells (BMSCs). Hence, there is an urgent need for improving antioxidative-stress processes of transplanted BMSCs to further promote their survival. The present study reports the role and mechanism of Parkinson's disease protein 7 (PARK7) in enhancing antioxidative activity in BMSCs.

Preincubation with a low-dose hydrogen peroxide enhances anti-oxidative stress ability of BMSCs.

Objective: To investigate the effects of low-concentration hydrogen peroxide pretreatment on the anti-oxidative stress of the bone marrow mesenchymal stem cells (BMSCs).

Methods: Rabbit BMSCs were isolated and cultured by density gradient centrifugation combined with the adherence method. Then, the third generation of well-grown BMSCs was continuously treated with 50-μM hydrogen peroxide (HO) for 8 h as the optimal pretreatment concentration and the BMSCs were continuously applied for 24 h with 500 μM HO, and the optimal damage concentration was determined as the oxidative stress cell model.

[Effects of nicotinamide mononucleotide adenylyl transferase 3 on mitochondrial function and anti-oxidative stress of rabbit bone marrow mesenchymal stem cells via regulating nicotinamide adenine dinucleotide levels].

Objective: To investigate the effect of nicotinamide mononucleotide adenosyl transferase 3 (NMNAT3) on the mitochondrial function and anti-oxidative stress of rabbit bone marrow mesenchymal stem cells (BMSCs) under oxidative stress by regulating nicotinamide adenine dinucleotide (NAD ) levels.

Methods: The bone marrow of femur and tibia of New Zealand white rabbits were extracted. BMSCs were isolated and cultured by density gradient centrifugation combined with adherent culture.

New strategy of bone marrow mesenchymal stem cells against oxidative stress injury via Nrf2 pathway: oxidative stress preconditioning.

Clinically, bone marrow mesenchymal stem cells (BMSCs) have been used in treatment of many diseases, but the local oxidative stress (OS) of lesion severely limits the survival of BMSCs, which reduces the efficacy of BMSCs transplantation. Therefore, enhancing the anti-OS stress ability of BMSCs is a key breakthrough point. Preconditioning is a common protective mechanism for cells or body.

A novel fiber optical device for ultraviolet disinfection of water.

Since there are several problems in traditional UV disinfection techniques, a highly efficient, reliable and economical method, using quartz optical fibers to deliver UV light is proposed. The principle of the experimental setup is that ultraviolet rays are gathered by a reflector and converge on a light point, the diameter of approximately 5mm. In this way UV light can be transferred into water to kill the bacteria in the water.

Thirty-six years experience of cervical extension osteotomy in ankylosing spondylitis: techniques and outcomes.

Study Design: A retrospective review of the cervical extension osteotomy in the past 36 years for the treatment of flexion deformity of patients with ankylosing spondylitis was conducted.

Objectives: To review the conventional and current surgical techniques of cervical extension osteotomy in ankylosing spondylitis and to evaluate the clinical outcomes.

Summary Of Background Data: Cervical osteotomy is a challenging procedure in the correction of flexion deformity in ankylosing spondylitis.

Vertebral tumors: surgical versus nonsurgical treatment.

Unlabelled: The treatment of spinal tumors represents a challenge to spine care professionals. Fortunately, the incidence of new cases of primary malignant bone tumors is lower compared with that of other tumors. In the United States approximately 2000 malignant bone tumors of 7000 new sarcomas are diagnosed each year.

Value of magnetic resonance imaging and discography in determining the level of cervical discectomy and fusion.

Study Design: The correlation between magnetic resonance imaging and discography of the cervical spine in degenerative disc disease was studied. In addition, the results of cervical discectomy and fusion were evaluated.

Objectives: To compare the value of cervical magnetic resonance imaging versus discography in selecting the level for discectomy and fusion and to evaluate the surgical outcome.