Simultaneous detection of diethylstilbestrol and estradiol residues with a single immunochromatographic assay strip.

An immunochromatographic assay (ICA) based on competitive format was developed and validated for simultaneously rapid and sensitive detection of diethylstilbestrol (DES) and estradiol (E) in milk and tissue samples. For this purpose, two monoclonal antibodies raised against those two estrogens were conjugated to gold nanoparticles and were applied to the conjugate pads of the test strip. The competitors of the DES-BSA/E-BSA conjugates were immobilized onto a nitrocellulose membrane at two detection zones to form T and T, respectively.