Publications by authors named "Yingchen Hao"
Article Synopsis
- Phenolamides are important plant metabolites that help tomatoes adapt to stress, but their metabolism and regulation are not well understood, particularly in the context of breeding and domestication.
- The study identified two gene clusters involved in phenolamide metabolism and found that a specific gene (SlMYB13) helps regulate their accumulation, which in turn enhances drought tolerance in tomatoes.
- Findings indicate that during the domestication process, key genes related to phenolamide production were negatively selected, resulting in reduced phenolamide levels and drought resistance in cultivated tomatoes.
Specialized plant metabolism is a rich resource of compounds for drug discovery. The acylated flavonoid glycoside melitidin is being developed as an anti-cholesterol statin drug candidate, but its biosynthetic route in plants has not yet been fully characterized. Here, we describe the gene discovery and functional characterization of a new flavonoid gene cluster (UDP-glucuronosyltransferases (CgUGTs), 1,2 rhamnosyltransferase (Cg1,2RhaT), acyltransferases (CgATs)) that is responsible for melitidin biosynthesis in pummelo (Citrus grandis (L.
View Article and Find Full Text PDFSteroidal glycoalkaloids (SGAs) constitute a characteristic class of antinutritional metabolites that are found in certain Solanum species. Despite the considerable studies on SGA biosynthesis, the mechanisms of crosstalk between hormone signaling pathways that regulate SGA content still remain to be elucidated. Here, we performed a metabolic genome-wide association study (mGWAS) based on the levels of SGA metabolites and identified SlERF.
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- - The study investigated the role of DNA methylation, an epigenetic marker, in tomato breeding by analyzing wild tomatoes, landraces, and cultivars through whole-genome sequencing and metabolic profiling, identifying 8,375 differentially methylated regions (DMRs).
- - It was found that DNA methylation levels decrease from domestication to improvement, with over 20% of DMRs overlapping with selective sweeps and many being linked to adjacent SNPs rather than being directly associated with them.
- - The research identified 13 candidate genes and a more detailed polyphenol biosynthetic pathway, showcasing that DNA methylation can enhance our understanding of metabolite diversity in plants alongside traditional SNP profiling.
Steroidal alkaloids (SAs) and steroidal glycoalkaloids (SGAs) are common constituents of plant species belonging to the family. However, the molecular mechanism regulating the formation of SAs and SGAs remains unknown. Here, genome-wide association mapping was used to elucidate SA and SGA regulation in tomatoes: a SlGAME5-like glycosyltransferase (Solyc10g085240) and the transcription factor SlDOG1 (Solyc10g085210) were significantly associated with steroidal alkaloid composition.
View Article and Find Full Text PDFGeneral control nonderepressible 2 (GCN2) can phosphorylate the α subunit of eukaryotic initiation factor eIF2 (eukaryotic translation initiation factor 2) to down-regulateprotein synthesis in response to various biotic and abiotic stresses. However, the kinase activity of plant GCN2 has not been well-characterized in vitro. In this study, the kinase domain of Nicotiana tabacum GCN2 (NtGCN2) was inserted into the pET15b vector for prokaryotic expressionin Escherichia coli BL21-CodonPlus-(DE3)-RIPL after induction by 0.
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