Rice Reference Genes (RRG): redefining reference genes in rice by mining RNA-seq datasets.

Reverse transcription quantitative real-time PCR (RT-qPCR) is esteemed for its precision and reliability, positioning it as the standard for evaluating gene expression. Selecting suitable reference genes is crucial for acquiring accurate data on target gene expression. However, identifying appropriate reference genes for specific rice tissues or growth conditions has been a challenge.

The Role of Iron in Atherosclerosis and its Association with Related Diseases.

Purpose Of Review: This review aims to elucidate the multifaceted role of iron in the pathogenesis of atherosclerosis. The primary objective is to summarize recent advances in understanding how iron contributes to atherosclerosis through various cellular mechanisms. Additionally, the review explores the therapeutic implications of targeting iron metabolism in the prevention and treatment of cardiovascular diseases.

OsRAV1 Regulates Seed Vigor and Salt Tolerance During Germination in Rice.

Seed vigor is a complex trait encompassing seed germination, seedling emergence, growth, seed longevity, and stress tolerance, all are crucial for direct seeding in rice. Here, we report that the AP2/ERF transcription factor OsRAV1 (RELATED TO ABI3 AND VP1) positively regulates seed germination, vigor, and salt tolerance. Additionally, OsRAV1 was differently expressed in embryo and endosperm, with the OsRAV1 localized in the nucleus.

Transcriptomic analysis of salt-tolerant and sensitive high-yield japonica rice (Oryza sativa L.) reveals complicated salt-tolerant mechanisms.

Developing and cultivating rice varieties is a potent strategy for reclaiming salinity-affected soils for rice production. Nevertheless, the molecular mechanisms conferring salt tolerance, especially in conventional high-yield japonica rice varieties, remain obscure. In this study, Zhendao 23309 (ZD23309) exhibited significantly less grain yield reduction under a salt stress gradient than the control variety Wuyunjing 30 (WYJ30).

Heat stress and sexual reproduction in maize: unveiling the most pivotal factors and the greatest opportunities.

The escalation in the intensity, frequency, and duration of high-temperature (HT) stress is currently unparalleled, which aggravates the challenges for crop production. Yet, the stage-dependent responses of reproductive organs to HT stress at the morphological, physiological, and molecular levels remain inadequately explored in pivotal staple crops. This review synthesized current knowledge regarding the mechanisms by which HT stress induces abnormalities and aberrations in reproductive growth and development, as well as by which it alters the morphology and function of florets, flowering patterns, and the processes of pollination and fertilization in maize (Zea mays L.

Validation of Novel Reference Genes in Different Rice Plant Tissues through Mining RNA-Seq Datasets.

Reverse transcription quantitative real-time PCR (RT-qPCR) is arguably the most prevalent and accurate quantitative gene expression analysis. However, selection of reliable reference genes for RT-qPCR in rice () is still limited, especially for a specific tissue type or growth condition. In this study, we took the advantage of our RNA-seq datasets encompassing data from five rice varieties with diverse treatment conditions, identified 12 novel candidate reference genes, and conducted rigorous evaluations of their suitability across typical rice tissues.

From the floret to the canopy: High temperature tolerance during flowering.

Heat waves induced by climate warming have become common in food-producing regions worldwide, frequently coinciding with high temperature (HT)-sensitive stages of many crops and thus threatening global food security. Understanding the HT sensitivity of reproductive organs is currently of great interest for increasing seed set. The responses of seed set to HT involve multiple processes in both male and female reproductive organs, but we currently lack an integrated and systematic summary of these responses for the world's three leading food crops (rice, wheat, and maize).

[Optimizing Straw Management to Enhance Carbon and Nitrogen Efficiency and Economic Benefit of Wheat-Maize Double Cropping System].

The optimization of annual straw management can improve the yield, income, and carbon and nitrogen efficiency of wheat-maize double cropping systems. Based on a long-term positioning trial started in 2012, five straw management methods were considered, C100 (100% return), C75 (75% return+25% harvest), C50 (50% return+50% harvest), C25 (25% return+75% harvest), and C0 (100% harvest). We analyzed the effects of farmland carbon and nitrogen inputs and their ratios on crop yield, carbon and nitrogen use efficiency, and economic benefits in wheat and maize anniversaries with different straw managements.

Numerical study of aeolian vibration characteristics and fatigue life estimation of transmission conductors.

The 2D computational fluid dynamics (CFD) model of transmission conductor is set up to simulate the aerodynamic forces varying with time on the conductor. Taking into account the geometrical nonlinearity of conductor lines, the finite element (FE) models of single span and two-span transmission lines discretized with beam elements are established. By means of the FE models, the aeolian vibrations of the conductor lines excited by the aerodynamic forces under different wind velocities are numerically simulated.

Interaction Effects of Nitrogen Rates and Forms Combined With and Without Zinc Supply on Plant Growth and Nutrient Uptake in Maize Seedlings.

Previous studies have shown that zinc (Zn) accumulation in shoot and grain increased as applied nitrogen (N) rate increased only when Zn supply was not limiting, suggesting a synergistic effect of N on plant Zn accumulation. However, little information is available about the effects of different mineral N sources combined with the presence or absence of Zn on the growth of both shoot and root and nutrient uptake. Maize plants were grown under sand-cultured conditions at three N forms as follows: NO nutrition alone, mixture of NO /NH with molar ratio of 1:1 (recorded as mixed-N), and NH nutrition alone including zero N supply as the control.

Elevated atmospheric CO concentration triggers redistribution of nitrogen to promote tillering in rice.

Elevated atmospheric CO concentration (eCO) often reduces nitrogen (N) content in rice plants and stimulates tillering. However, there is a general consensus that reduced N would constrain rice tillering. To resolve this contradiction, we investigated N distribution and transcriptomic changes in different rice plant organs after subjecting them to eCO and different N application rates.

Transcriptomic and Co-Expression Network Profiling of Shoot Apical Meristem Reveal Contrasting Response to Nitrogen Rate between and Rice Subspecies.

Reducing nitrogen (N) input is a key measure to achieve a sustainable rice production in China, especially in Jiangsu Province. Tiller is the basis for achieving panicle number that plays as a major factor in the yield determination. In actual production, excessive N is often applied in order to produce enough tillers in the early stages.

Global Transcriptome and Co-Expression Network Analysis Reveal Contrasting Response of and Rice Cultivar to γ Radiation.

and are two important subspecies in cultivated Asian rice. Irradiation is a classical approach to induce mutations and create novel germplasm. However, little is known about the differential response between and rice after γ radiation.

[Study on the optimal extaction of total flavonoids from Tagetes erecta by central composite design-response surface methodology].

Objective: To optimize the process of extracting total flavonoids from Tagetes erecta.

Methods: The influential factors were extraction temperature, ethanol concentration, reflux time and solvent volume fold. The evaluating indicator was the extraction rate of total flavonoids from Tagetes erecta.