A two-color fluorescence sensing strategy based on functionalized tetrahedral DNAzyme nanotweezers for ochratoxin A detection.

A two-color fluorescent sensing strategy based on a functionalized tetrahedral DNAzyme nanotweezer (FTDN) was developed to detect ochratoxin A (OTA) utilizing the multifunctional properties of DNA nanotechnology. The FTDN enables rapid OTA detection directly through a Cy5 fluorescent group, modified to respond to the target signal. Additionally, FTDN exhibits DNAzyme cutting activity in the presence of Mg ions, enabling it to traverse DNA nanoflower-functionalized magnetic beads.

Propylparaben Induces Reproductive Toxicity in Human Extravillous Trophoblast Cells via Apoptosis and Cell Cycle Pathways.

Parabens (PBs), especially propylparaben, commonly used in consumer products, pose environmental and health concerns. This study explored propylparaben's cytotoxicity on HTR-8/SVneo human trophoblast cells, revealing significant dose-dependent cytotoxic effects, particularly post 48-h exposure. Elevated propylparaben levels triggered apoptosis, evidenced by increased Bax and activated Caspase-3, and induced the G0/G1 cell cycle arrest.

Insights into preeclampsia: a bioinformatics approach to deciphering genetic and immune contributions.

Background: Preeclampsia (PE) is a global pregnancy concern, characterized by hypertension with an unclear etiology. This study employs Mendelian randomization (MR) and single-cell RNA sequencing (scRNA-seq) to clarify its genetic and molecular roots, offering insights into diagnosis and treatment avenues.

Methods: We integrated PE-specific genome-wide association study (GWAS) data, expression and protein quantitative trait loci (eQTL and pQTL) data, and single-cell data from peripheral blood mononuclear cells (PBMCs).

A colorimetric/electrochemical microfluidic biosensor using target-triggered DNA hydrogels for organophosphorus detection.

Organophosphorus compounds are widely distributed and highly toxic to the environment and living organisms. The current detection of organophosphorus compounds is based on a single-mode method, which makes it challenging to achieve good portability, accuracy, and sensitivity simultaneously. This study designed a multifunctional microfluidic chip to develop a dual-mode biosensor employing a DNA hydrogel as a carrier and aptamers as recognition probes for the colorimetric/electrochemical detection of malathion, an organophosphorus compound.

Programmable DNA tweezers-SDA for ultra-sensitive signal amplification fluorescence sensing strategy.

Background: DNA tweezers, classified as DNA nanomachines, have gained prominence as multifunctional biosensors due to their advantages, including a straightforward structure, response mechanism, and high programmability. While the DNA tweezers demonstrate simultaneous, rapid, and stable responses to different targets, their detection sensitivity requires enhancement. Some small molecules, such as mycotoxins, often require more sensitive detection due to their extremely high toxicity.

Machine learning assisted biosensing technology: An emerging powerful tool for improving the intelligence of food safety detection.

Recently, the application of biosensors in food safety assessment has gained considerable research attention. Nevertheless, the evaluation of biosensors' sensitivity, accuracy, and efficiency is still ongoing. The advent of machine learning has enhanced the application of biosensors in food security assessment, yielding improved results.

MOF-on-MOF heterostructure boosting AIE sensing and triggered structural collapse for histamine detection.

We explored a novel preparation method for MOF-on-MOF heterostructured material (Zn-BTEC@ZIF-8). This prepared heterostructured material acts as a container, capable of adsorbing tetracycline hydrochloride molecules into its backbone through hydrogen bonding and π-π interactions. This phenomenon triggers an aggregation induced emission (AIE) effect, leading to the formation of luminescent bodies.

Dysregulation of AMPK-mTOR signaling leads to comorbid anxiety in Dip2a KO mice.

Autism is often comorbid with other psychiatric disorders. We have previously shown that Dip2a knockout (KO) induces autism-like behaviors in mice. However, the role of Dip2a in other psychiatric disorders remains unclear.

Antagonistic Activity and Mechanism of XZ16-1 Suppression of Wheat Powdery Mildew and Growth Promotion of Wheat.

Wheat powdery mildew caused by f. sp. () is one of the most serious wheat diseases in the world.