Publications by authors named "Ying-zi Yu"
Objective: To investigate the mechanism of apoptosis of chronic myeloid leukemia (CML) cells induced by the novel p210 bcr/abl inhibitor berbamine.
Methods: Human Ph+ CML leukemia K562 cells, which express endogenous p210 bcr/abl protein, were cultured in RPMI 1640 and treated with berbamine as indicated time and dose. Flow cytometry (FCM) and Annexin-V-Fluos/PI staining kit were used to evaluate the apoptosis of leukemic cells; FCM and cytoperm/cytofix plus Caspase-3-McAb-PE were employed to measure the leukemic cells with activated Caspase-3.
Article Synopsis
- - The study aimed to assess the levels of Shp-2 tyrosine phosphatase in chronic myeloid leukemia (CML) and how it relates to the cancer cells' ability to grow uncontrollably and resist apoptosis.
- - Researchers collected samples from 25 CML patients alongside healthy control samples to evaluate Shp-2 expression using Western blotting and monitored cell apoptosis through flow cytometry after manipulating Shp-2 levels.
- - The findings showed that phosphorylated Shp-2 was significantly overexpressed in 92% of CML samples compared to normal cells, indicating a potential link to the malignancy's aggressive growth and survival characteristics.