MicroRNA let-7a inhibits the proliferation and invasion of nonsmall cell lung cancer cell line 95D by regulating K-Ras and HMGA2 gene expression.

MicroRNAs are closely linked to tumor metastasis and let-7a may play a role in inhibiting the proliferation, invasion, and metastasis of lung cancer. In vitro, we aim to observe the impact of let-7a on the proliferation and invasion of the nonsmall cell lung cancer cell line 95D by constructing a lentiviral vector that expresses let-7a. Cell proliferation assays and Transwell experiments were used to compare the proliferation and invasion of the 95D cell group with let-7a overexpressed or inhibited.

The variation of CD4+CD25+ regulatory T cells in the periphery blood and tumor microenvironment of non-small cell lung cancer patients and the downregulation effects induced by CpG ODN.

The aim of the study was to observe the variation of CD4(+)CD25(+) regulatory T cells in periphery blood and tumor microenvironment of non-small cell lung cancer (NSCLC) patients and the effects of CpG ODN. The proportion of CD4(+)CD25(+) regulatory T cells, Foxp3 gene expression, levels of tumor growth factor-β (TGF-β) and immunoreactive fibronectin-γ (IFN-γ) in the periphery blood of 30 NSCLC patients and 30 healthy volunteers were compared. These indicators were compared before and after CpG ODN treatment.

[The effect of lipopolysaccharide on gene expression of TLR4 and MD-2 in rat alveolar macrophage and its secretion of inflammation cytokines].

Objective: To investigate the effect of lipopolysaccharide (LPS) on gene expression of Toll-Like receptor 4 (TLR4) and myeloid differentiation protein-2 (MD-2) in rat alveolar macrophage NR8383 cell and its secretion of inflammation cytokines with or without small interference RNA target MD-2 (MD2-siRNA) treatment.

Methods: NR8383 cell was cultured with F-12K medium and stimulated with LPS (0.01 approximately 10 mg/L) for 2 h or stimulated with 1 microg/ml LPS for 2-24 h.

Continuous tracheal gas insufflation during protective mechanical ventilation in juvenile piglets with acute lung injury induced by endotoxin.

Background: Low tidal volume mechanical ventilation is difficult to correct hypoxemia, and prolonged inhalation of pure oxygen can lead to oxygen poisoning. We suggest that continuous tracheal gas insufflation (TGI) during protective mechanical ventilation could improve cardiopulmonary function in acute lung injury.

Methods: Totally 12 healthy juvenile piglets were anesthetized and mechanically ventilated at PEEP of 2 cmH2O with a peak inspiratory pressure of 10 cmH2O.

[Modulation of TLR9 on anti-tumor immune responses of peripheral blood mononuclear cells from patients with non-small-cell lung cancer].

Objective: To assess the modulation of TLR9 on anti-tumor immune responses in peripheral blood mononuclear cells (PBMC) from patients with non-small-cell lung cancer (NSCLC).

Methods: PBMCs were isolated from 36 NSCLC patients. Lung cancer cells were isolated from these patients and further enriched.

Targeting toll-like receptor 9 with CpG oligodeoxynucleotides enhances anti-tumor responses of peripheral blood mononuclear cells from human lung cancer patients.

CpG-oligonucleotides (CpG-ODN), which induce signaling through Toll-like receptor 9 (TLR9), are currently under investigation as adjuvants in therapy against infections and cancer. However, whether the CpG-ODN alone could enhance the anti-tumor immunity and the underlying mechanisms remains unclear. Here, we investigated that stimulation of peripheral blood mononuclear cells (PBMCs) from human lung cancer patients with CpG-ODN induced proliferation responses of the PBMCs, accompanied by the elevated cytokine secretion, including IFN-alpha, IL-12 and TNF-alpha.

[Determination and clinical implication of leukotriene B(4) and tumor necrosis factor-alpha in condensate of exhaled breath of chronic obstructive pulmonary disease patients].

Objective: To study the changes and clinical implication of leukotriene B(4) (LTB(4)) and tumor necrosis factor-alpha (TNF-alpha) in exhaled breath condensate (EBC) of chronic obstructive pulmonary disease (COPD) patients.

Methods: EBC of 20 patients in acute episode of COPD (AECOPD), 20 patients in period of remission of COPD, and 20 persons who were having regular check-up (healthy control group) were enrolled. The concentrations of LTB(4) and TNF-alpha in EBC were assayed.

[A study on the mechanisms of mycobacterial clearance induced by CpG-oligodeoxynucleotides in mice].

Objective: To investigate the possible mechanisms of mycobacterial clearance induced by CpG-oligodeoxynucleotides (CpG-ODN) in mice.

Methods: Eight-week-old female BALB/c mice were treated with intraperitoneal CpG-ODN (30 microg), while the control mice with normal saline. After 2 weeks, the control mice and the CpG-treated mice were infected with Mycobacterium tuberculosis (1 x 10(6) colony-forming units, H(37)Rv strain) through the tail vein.

[A study on the recurrence of ischemic stroke in nonvalvular atrial fibrillation].

Objective: To study the recurrence of ischemic stroke in patient with nonvalvular atrial fibrillation (NVAF) and to determine the recurrence related factors of ischemic stroke in patients with NVAF.

Methods: The clinical data of 386 NVAF patients with ischemic stroke treated in our hospital from January 1992 to February 2002 were included in this study. The basic clinical information of each patient was recorded.

[Development and application of the software for analysis of arterial blood gases in treatment suggestions and training].

Objective: Suggestions and training in developing a computer software for analysis of arterial blood gases in treatment to be used in clinic and teaching.

Methods: The software was edited by means of Delphi 7.0 and closely combined with Windows operating system.

[The effects of CpG-oligodeoxynucleotides on airway remodeling in chronic asthmatic mice].

Objective: To investigate if CpG-oligodeoxynucleotides (CpG-ODN) intervention has inhibitory effects on the development of airway remodeling in an ovalbumin (OVA)-sensitized mouse model of chronic asthma.

Methods: Forty female C57BL/6 mice were randomly divided into four groups (n = 10): (1) Group A (chronic asthma model): mice were sensitized by intraperitoneal injection of OVA (10 microg) precipitated with aluminium hydroxide (100 microg) on days 1 and 14. From day 21, the mice were challenged by nebulized 2.

[Therapeutic effects and mechanisms of CpG oligodeoxynucleotides in mice infected with Mycobacterium tuberculosis].

Objective: To investigate the protective effects and mechanisms of CpG oligodeoxynucleotides (CpG ODN) in mice infected with Mycobacterium tuberculosis.

Methods: Ninety-six female BALB/c mice were randomized into 4 groups, namely CpG ODN treatment (A group), control ODN treatment (B group), infection control (C group) and healthy control (D group) (n = 24 each). A group and B group were treated once with CpG ODN or control ODN (30 microg/mouse) intraperitoneally 2 weeks before infection with Mycobacterium tuberculosis.

[The combined effect of isoniazid and rifampicin on the activities of CYP4501A2 and CYP4503A4 in primary hepatocytes from healthy human adults].

Objective: To study the combined effect of isoniazid and rifampicin on the activities of CYP1A2 and 3A4 in primary hepatocytes from healthy human adults.

Methods: The primary hepatocytes were isolated from adult healthy human livers, and cultured for 3 days. Then the cells were divided into 15 groups including two negative control groups (culture media alone) and 13 drug intervention groups, to which the following drugs were added: isoniazid (25 micromol/L, 50 micromol/L), rifampicin (12.

[Correlation between the area of arterial blood gases graph and the acid-base disturbance in 92 cases with chronic obstructive pulmonary disease].

Objective: To study the correlation between the area of arterial blood gases graph (ABGG) and the acid-base disturbance (ABD).

Methods: Using the software developed by ourselves, the results of arterial blood gases and concomitant electrolyte (K(+), Na(+), Cl(-), HCO(3)(-)) determination in 313 episodes were analyzed in 92 cases with chronic obstructive pulmonary diseases (COPD) during hospitalization, and the correlation was studied.

Results: (1)On admission, among 92 cases, in 9 cases the results of blood gas analysis were in the area of compensated ventilation and deranged gas exchange (area IV), in 82 cases the results were in the area of insufficient ventilation and deranged gas exchange (area V), and 1 case in the area of excessive ventilation and deranged gas exchange (area VI).

[The expression of survivin messenger RNA in sputum and cancerous tissue in human lung cancer].

Objective: To study the diagnostic significance of the expression of survivin mRNA in transbronchial biopy samples and sputum samples in lung cancer.

Methods: The resected lung cancer tissues and their para-carcinomatous normal tissue specimens of 41 patients with lung cancer and tissue specimens of 9 patients with benign pulmonary diseases were studied. 110 bronchial biopsy specimens from 80 patients with lung cancer and 30 patients with benign pulmonary diseases and their 160 sputum samples were also evaluated.

[The relationship between vitamin D binding protein gene polymorphism and chronic obstructive pulmonary disease].

Objective: To determine the association between vitamin D binding protein (VDBP) gene and chronic obstructive pulmonary disease (COPD) susceptibility in Han Nationality in China.

Methods: This was a case-control study. Blood samples were taken from sixty-nine smokers with COPD (group A) and fifty-two smokers without COPD (Group B).

[Development and its clinical application of an acid-base balance computer software].

Objective: To develop acid-base balance computer software for estimating acid-base disturbance rapidly and accurately.

Methods: The data module was set up, based on Henderson-Hasselbalch (H-H) and compensation formula, and the program was written with Delphi 5.0.

[A retrospective study on the treatment of patients with chronic obstructive pulmonary disease by face mask mechanical ventilation].

Objective: To assess the effect and the influencing factors in face mask mechanical ventilation (FMMV) for the treatment of COPD with respiratory failure (RF), and to compare the efficacy and efficiency of FMMV with endotracheal intubation mechanical ventilation (ETMV).

Methods: From 1991 to 2001, 4 ventilating periods were named initial, middle, late, and extending stage according to ventilators, face masks and mechanical ventilating methods. The results of FMMV in the 4 periods were compared.

[Clinical application of a software for the analysis of arterial blood gases graph in 231 patients with chronic obstructive pulmonary disease].

Objective: To evaluate the clinical significance of a computer software for the analysis of arterial blood gases graph (ABGG) in chronic obstructive pulmonary disease(COPD).

Methods: The software was developed with Win98 as the operating platform and the visual software Delphi 5.0 from Borland Company, and it was used for evaluation of the changes in arterial blood gases (ABG) of 231 COPD cases.