Publications by authors named "Ying-lei Ji"
Objective: To explore the role of hydrogen sulfide (H2S) in acute liver injury induced by crushing hind limbs of rats.
Methods: The rats were randomly divided into the following groups: control, crushing, H2S donor sodium hydrosulfide (NaHS) + crushing, H2S inhibitor propargylglycine (PAG) + crushing group. The acute liver injury model was established by 'crushing the hind limbs of rats with standard weight.
Article Synopsis
- - The study examines how the stress protein heme oxygenase-1 (HO-1) impacts endoplasmic reticulum stress (ERS) in rat liver cells (hepatocytes) when exposed to lipopolysaccharide (LPS).
- - Researchers treated cultured rat hepatocyte cells with various combinations of LPS and HO-1 interference methods, measuring cell health and expression of specific stress-related proteins.
- - Findings indicated that LPS increased HO-1 levels and worsened cell injury and apoptosis; however, blocking HO-1 with siRNA led to even more severe ERS and cell damage, suggesting that HO-1 plays a protective role against LPS-induced injury.
Objective: To investigate the role of endoplasmic reticulum stress (ERS) in lipopolysaccharide (LPS)-induced hepatocyte apoptosis.
Methods: Cells of the rat hepatocyte line BRL were cultured. The hepatocytes were treated with LPS, ERS inducer thapsigargin (TG), and ERS inhibitor 4-phenylbutyric acid (4-PBA), respectively or in their different combination.