Publications by authors named "Ying-Zhuo Lin"
Objective: To explore the effect of syndecan-1 (Sdc-1) in the pathogenesis of colitis.
Methods: Thirty BALB/c mice were forced to drink 4% dextran sodium sulphate (DSS) in distilled water as the sole source of drinking fluid for 7 days, distilled water for 10 days, and 4% DSS in distilled water for another 7 days so as to establish colitis models and then were randomly divided into 3 equal groups: model groups 1, 2, and 3 to be killed 8, 18, and 25 days after the DSS drinking respectively to take their colons. Another 10 mice were fed with distilled water as control group and were killed on Day 8.
Objective: To explore the protective effect of epigallocatechin-3-gallate (EGCG) on ulcerative colitis (UC) and mechanism thereof.
Methods: Sixty SD rats underwent enema of trinitrobenzene sulfonic acid (TNBS) to cause UC and then randomly divided into 6 groups: model group, undergoing enema of normal saline (NS) once a day for 2 weeks; positive drug control group, undergoing enema of 5-aminosalicylic acid (ASA), an anti-UC drug; high-dose EGCG group, undergoing enema of 100 mg/kg EGCG; low-dose EGCG group, undergoing 5 enema of 50 mg/kg EGCG; and EGCG pretreatment group, undergoing enema of 100 mg/kg once a day 3 days before the model establishment and then once a day for 2 weeks after the model establishment. Another 12 rats were used as normal controls.
Objective: To investigate the effect of short hairpin RNA (shRNA) targeting survivin on adhesion and invasion of human colon carcinoma cell line SW480 in vitro.
Methods: According to the sequence of the coding region of survivin gene, two strings of 19 nucleotides of inverted sequence flanking the loop sequence of two complementary 9-base oligonucleotides were designed and synthesized to prepare the hairpin construct as the DNA templates for the target shRNA. The shRNA templates were cloned into shRNA expression vector pRNAT-U6.