Expression of gene in ovarian cancer and its impact on the biologic function of ovarian cancer cells.

The aim of the present study was to evaluate the expression of the chemokine ligand 18 () gene in ovarian cancer and to investigate the effects of its overexpression or suppression on growth, invasion, and metastasis in an ovarian carcinoma cell line (SKOV3) . mRNA expression in epithelial ovarian carcinoma (EOC), benign ovarian tumor and normal ovarian tissues was measured by fluorescence quantitative polymerase chain reaction. A restructuring plasmid was constructed, and SKOV3 cells were transfected with the plasmid DNA .

[Study of growth, invasion and metastasis on ovarian epithelial cancer cell line with CCL18 over-expression by mediated in vitro].

Objective: To investigate the effect of growth, invasion and metastasis on ovarian cancer cell line SKOV3 with chemokine (C-C motif) ligand 18 (CCL18) over-expression by mediated in vitro.

Methods: The restructuring plasmid of CCL18 expression was constructed and SKOV3 cells was transfected with plasmid DNA in vitro. The growth curve, cell cycle, cell migration, invasion and adhesion capacity in SKOV3-CCL18 cells and control cells were detected by methyl thiazolyl tetrazolium (MTT) assay, flow cytometry, transwell chamber, migration invasion and fibronectin adhesion method, respectively.