[Study on the chemical constituents of Clerodendron philippinum var. simplex].

Objective: To study the chemical constituents of Clerodendron philippinum var. simplex.

Methods: The ethanol extract of Clerodendron philippinum var.

Nodulisporisteriods A and B, the first 3,4-seco-4-methyl-progesteroids from Nodulisporium sp.

Two new 4-methyl-progesteroids, nodulisporisteriod A (1) and nodulisporisteriod B (2), were isolated from the extract of an endolichenic fungal strain Nodulisporium sp. (No. 65-17-2-1), along with two related metabolites, demethoxyviridin (3) and inoterpene B (4).

Xanthoquinodins from the endolichenic fungal strain Chaetomium elatum.

Five new xanthoquinodins, A4-A6 (1-3), B4 (4), and B5 (5), were isolated from the crude extract of the endolichenic fungal strain Chaetomium elatum (No. 63-10-3-1), along with three known xanthoquinodins, A1-A3 (6-8). Their structures were determined by detailed spectroscopic analysis and comparison of the NMR data with those of the closely related compounds previously reported.

[The role of microRNAs in the pathogenesis of cervical cancer and its relationship to HPV].

A lot of miRNAs have participated in the regulation of numerous biological processes such as cell proliferation, apoptosis, invasion and migration in cervical cancer, and closely associated with prognosis and susceptibility of cervical cancer. The oncogenic proteins E6, E7 and E5 expressed by HPV directly or indirectly lead to the dysregulation of multiple miRNAs such as miR-34a,miR-218, miR-29a and miR-146a, subsequently contribute to the initiation and progression of cervical cancer. In turn, some miRNAs such as miR-203 and miR-125b also play roles in the regulation of HPV DNA duplication.

New azaphilones and chlorinated phenolic glycosides from Chaetomium elatum with caspase-3 inhibitory activity.

Three new azaphilones, chaetomugilin S (1), 7,5'-bis-epi-chaetoviridin A (2), and 7-epi-chaetoviridin E (3), and two new chlorinated phenolic glycosides, globosumoside A (4) and globosumoside B (5), were isolated from the crude extract of the fungal strain Chaetomium elatum No. 89-1-3-1. Their structures were determined by detailed NMR and MS spectroscopic analyses.

[Chemical constituents of Berchemia lineate].

Objective: To investigate the chemical constituents of the roots of Berchemia lineate as a medicinal plant of Yao nationality in China.

Methods: Compounds were isolated by various column chromatography and elucidated by physicochemical and spectral analysis.

Results: Nine compounds were isolated from the 95% ethanol extract of the roots of Berchemia lineate and their structures were identified as palmitic acid (1), octadecanoic acid (2), beta-sitosterol (3), stigmasterol (4), fernenol (5), chrysophanol (6), physcion (7), floribundiquinone D (8), 2-acetylphyscion(9) respectively.

Two new diterpenoids from Croton crassifolius.

Two new clerodane-type diterpenoids were isolated from the roots of Croton crassifolius Geisel. The structures of these compounds were elucidated as 9-[2-(2(5H)-furanone-4-yl)ethyl]-4,8,9-trimethyl-1,2,3,4,5,6,7,8-octahydronaphthalene-4-carboxylic acid (1) and methyl 9-[2-(2(5H)-furanone-4-yl)ethyl]-4,8,9-trimethyl-1,2,3,4,5,6,7,8-octahydronaphthalene-4-carboxylic ester (2) by spectroscopic methods.

A new pregnane glycoside and oligosaccharide from Parabarium huaitingii.

Two new compounds, along with two known compounds, were isolated from the barks of Parabarium huaitingii, and their structures were determined as 5α-pregn-6-ene-3β,17α,20(S)-triol-20-O-β-d-digitoxopyranoside (1), cymaropyranurolactone 4-O-β-d-digitalopyranosyl-(1 → 4)-O-β-d-cymaropyranosyl-(1 → 4)-O-β-d-oleandropyranosyl-(1 → 4)-O-β-d-cymaropyranoside (2), 3β,17α,20(S)-trihydroxy-5α-pregn-6-ene (3), and 5α-pregn-6-ene-3β,17α,20(S)-triol-3-O-β-d-digitalopyranoside (4) by spectroscopic methods.

[Studies on the chemical constituents from Cissus pteroclada].

Objective: To study the chemical constituents of Yao Medicine Cissus pteroclada.

Methods: The compounds were isolated and purified by column chromatography with silica gel, TLC and recrystallization. Their structures were elucidated on the basis of physicochemical properties and spectra analysis.

[Studies on the triterpenoids constituents from Phyllodium elegans].

Objective: To study the chemical constituents of Phyllodium elegans.

Methods: The compounds were isolated and purified by extraction, chromatography on silica gel and recrystallization. Their structures were elucidated on the basis of physicochemical properties and spectra analysis.

[Studies on the chemical constituents of Blumea laciniata].

Objective: To study the chemical constituents of Blumea laciniata.

Methods: The chemical constituents of the ethyl acetate fraction of ethanol extract from Blumea laciniata were isolated with column chromatographic techniques. The structures of the isolated compounds were elucidated by spectroscopic analysis and comparison with their published datas.

[Studies on the chemical comstituents of Pithecellobium clypearia].

Objective: To study the chemical constituents of Pithecellobium clypearia.

Methods: The compounds were isolated from Pithecellobium clypearia with column chromatography. Their structures were characterized on the basis of their physical and chemical preperties, as well as chromatographic and spectroscopic evidences.

Chemical composition and antiproliferative activity of essential oil from the leaves of a medicinal herb, Schefflera heptaphylla.

Schefflera heptaphylla (L.) Frodin is a medicinal herb widely used as a main ingredient of the popular health tea formulation against infections in Southern China. Twenty-seven volatile compounds were identified by GC-MS analysis from the essential oil obtained from the leaves of S.

[Studies on the chemical constituents of Adina pilulifera].

The chemical constituents of Adina pilulifera, which were traditionlly used in the area of Yao minority in Southern China, were isolated and characterized. Two compounds were obtained from the ethyl acetate fraction of ethanol extract of Adina pilulifera, and one compound was obtained from the n-butanol fraction of ethanol extract of Adina pilulifera. Their structure were characterized by spectroscopic analysis and comparison with published data to be sarracenin (1), 2-methyl-5, 7-dihydroxychromone (2) and morroniside (3).

[Studies on antiproliferative effect of flavones compounds isolated from Yao herb medicines].

Objective: To detect the cytotoxicity and in vitro antiproliferative effects of the flavones compounds isolated from some Yao herbal medicines, and analyze the basic structure-activity relationship.

Methods: The cytotoxicity on normal cells and antiproliferative effect on tumor cells were tested by MTT reduction assay respectively.

Results: Among the 6 flavones, the Eriodictyol-7-O-a-D-glucoside, Naringenin-7-O-beta-D-glucoside, quercetin and quercetin-3-O-beta-D-glucoside showed very low cytotoxicity to the two normal cells, Vero and MC cells, while the naringenin was high toxic to both of them.

[Studies on chemical constituents from Serissa serissoides].

Objective: To study the chemical constituents of Serissa serissoides.

Method: Chemical constituents were isolated with the column chromatographic methods including silica gel and sephadex LH -20, and their structures were elucidated on the basis of their spectral and chemical evidences.

Result: Eight compounds were obtained and were identified as: palmitic acid (1), corosolic acid (2), daucosterol (3), urs-12-en-28-ol (4), oleanolic acid (5), uroslic acid (6), 4-hydroxy-3-methoxybenzoic acid (7), and 2,6-dimethoxy-p-benzoquinone (8).

[Study on the chemical constituents of Ardisia chinensis].

The chemical constituents of Ardisia chinensis, which traditionally used in the area of Yao minority in Southern China, were analyzed and characterized. Seven compounds were obtained from the ethyl acetate fraction of ethanol extract of Ardisia chinensis. These 7 compounds were characterized by spectroscopic analysis and comparison with published data to be salicylic acid (1), 4-hydroxy-3-methoxy-benzoic acid (2), gallic acid ethyl ester (3), 4-hydroxy-3,5-dimethoxy benzoic acid methyl ester (4), protocatechuic acid (5), gallic acid (6) and catechin (7).