Tracing the evolution of venom phospholipases A in Gloydius strauchii and related pitvipers: A tale of two acidic isozymes.

Two acidic Asp-PLAs with Glu substitution and a neutral Lys-PLA (designated Gst-K49) were cloned from G. strauchii venom glands, their full amino acid sequences were deduced. The predominant acidic PLA (designated Gst-E6a) contains 124 residues and the MW substitutions, while the minor acidic PLA (designated Gst-E6b) contains 122 residues and the VA substitutions.