Publications by authors named "Ying-Yu Deng"
[High expression of LIP1 in Pichia pastoris].
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai)
April 2003
Article Synopsis
- The LIP1 gene, crucial for producing a key enzyme in Candida rugosa, was artificially synthesized and cloned into two different expression vectors for testing in Pichia pastoris.
- The transformed yeast strains were compared, revealing that the strain with the pGAPZalphaA vector was more efficient at producing LIP1 than the one with pPICZalphaA.
- After optimizing fermentation conditions, the most successful strain, CHT-II, could secrete a significantly higher level of LIP1 into the supernate, confirming the success of the genetic modification.
Guinea pig maximization test for trichloroethylene and its metabolites.
Biomed Environ Sci
June 2002
Objectives: To study the contact allergenic activities of trichloroethylene (TCE) and its three metabolites trichloroacetic acid, trichloroethanol and chloral hydrate.
Methods: A modified guinea pig maximization test (GPMT) was adopted. The skin sensitization (edema and erythema) was observed in trichloroethylene, trichloroacetic acid, trichloroethanol, chloral hydrate and 2,4-dinitrochlorobenzene.
Objectives: To determine the possible relationship between plasma potassium concentration and severity of acute trimethyltin chloride (TMT) poisoning and to assess the mechanism of TMT induced hypokalemia.
Methods: SD rats were treated with various dosages of TMT (i.p.