The Combination of and Its Engineered Strain Expressing dsRNA Increases the Toxicity against .

RNA interference (RNAi) has been developed and used as an emerging strategy for pest management. Here, an entomopathogen () was used to express the dsRNA for the control of . A vector containing a 325-bp fragment of the conserved region of arginine kinase gene () flanking in two ends with the promoter Pro3α was developed and transferred into 8010 and BMB171, and consequently engineered strains 8010AKi and BMB171AKi expressing dsRNA of were developed.

Double-Stranded RNA-Degrading Enzymes Reduce the Efficiency of RNA Interference in .

DsRNA-degrading enzymes (dsRNases) have been recognized as important factors in reducing RNA interference (RNAi) efficiency in different insect species. However, dsRNases in are still unknown. We identified the full-length cDNAs of , , , and .