Effects of the combination of biochar and organic fertilizer on soil properties and agronomic attributes of soybean (Glycine max L.).

This research aimed to investigate the impacts of a combination of rice husk biochar and organic fertilizer on the physical and chemical properties of soil, the population of soil bacteria, the relative chlorophyll content of leaves, the development of soybean root nodules, and yield components under strongly acid soil conditions. A greenhouse and pot experiment was designed using a randomize complete block design with factorial 2 × 3 treatments and three replications. The experimental treatments comprised two rates of biochar (35 and 70 g/pot) and three rates of organic fertilizer (70, 105, and 140 g/pot).

Development of Rapid Detection Methods for f. sp. in Melon Seeds.

Melon ( L.) is a global commercial crop that is sensitive to seed-borne wilt infections caused by f. sp.

Rapid Detection of Using Insulated Isothermal PCR and a Rapid, Simple DNA Preparation Protocol.

We developed an insulated isothermal PCR (iiPCR) method for the efficient and rapid detection of (Fo), which is a fungus that infects various hosts and causes severe crop losses. The Fo iiPCR method was sensitive enough to detect up to 100 copies of standard DNA template and 10 fg of Fo genomic DNA. In addition, it could directly detect 1 pg of mycelium and 10 spores of Fo without DNA extraction.

Identification of Cyprodinil + Fludioxonil to Manage Soybean Red Crown Rot Using the Microplate-Based High-Throughput Screening and Pot Assay.

Red crown rot (RCR), caused by the soilborne fungus , is an emerging soybean disease in Taiwan, and fungicide screening is desired to identify effective management for . This study screened 11 fungicides, including azoxystrobin, boscalid, cyprodinil, cyprodinil + fludioxonil, difenoconazole, fluopyram, flutolanil, mancozeb, prochloraz, pyraclostrobin, and tebuconazole, for their inhibitory effects on the mycelial growth of 10 field isolates. Subsequently, a microplate-based high-throughput screening (MHTS) method was established to measure the fungicide sensitivity in a population composed of 80 isolates to three effective fungicides, cyprodinil + fludioxonil, fluopyram, and tebuconazole.

First report of leaf brown blight caused by Neopestalotiopsis formicarum on jabuticaba in Taiwan.

Jabuticaba (Plinia cauliflora (Mart.) Kausel) was originated from Brazil (Lorenzi 2000). The production of jabuticaba is growing globally as its value in the food and pharmaceutical industries (Benvenutti 2021).

Superoxide Initiates the Hyphal Differentiation to Microsclerotia Formation of .

The infection of often results in a grayish appearance with numerous survival structures, microsclerotia, on the plant surface. Past works have studied the development of fungal survival structures, sclerotia and microsclerotia, in the Leotiomycetes and Sordariomycetes. However, M.

Whole-Genome Sequence Resource of , the Casual Pathogen of Soybean Red Crown Rot.

(anamorph: ) is a soilborne plant-pathogenic fungus with a broad host range, and it can cause red crown rot of soybean and Cylindrocladium black rot of peanut, which has become an emerging threat to crop production worldwide. Limited molecular studies have focused on and one of the possible difficulties is the lack of genomic resources. This study presents the first high quality and near-completed genome of , using the Oxford Nanopore GridION sequencing platform.

Molecular Detection Assays for Rapid Field-Detection of Rice Sheath Blight.

(Rs), a soil-borne fungal pathogen, can result in rice sheath blight (ShB), which causes yield loss. To prevent outbreaks of ShB and enhance the sustainability of rice production, it is critical to develop a rapid ShB detection method for specific, fast, and on-site disease management. In this study, a reagent for the rapid extraction of this pathogen was developed for on-site detection.

Degenerated Virulence and Irregular Development of f. sp. Induced by Successive Subculture.

Successive cultivation of fungi on artificial media has been reported to cause the sectorization, which leads to degeneration of developmental phenotype, and virulence. f. sp.

First report of anthracnose caused by Colletotrichum fructicola on tea in Taiwan.

Tea (Camellia sinensis (L.) O. Kuntze) is a very popular beverage and cash crop that is widely cultivated in tropical and subtropical areas.

Construction of Raman spectroscopic fingerprints for the detection of Fusarium wilt of banana in Taiwan.

Banana (Musa sp.) is cultivated worldwide and is one of the most popular fruits. The soil-borne fungal disease Fusarium wilt of banana (FWB), commonly known as Panama disease, is caused by Fusarium oxysporum f.

Silver Nanoparticles on Nanoscale Silica Platelets (AgNP/NSP) and Nanoscale Silica Platelets (NSP) Inhibit the Development of f. sp. .

Nanotechnology has attracted much attention recently because of its agricultural applications. In this study, we analyzed the ability of two potential nanomaterials (NMs), nanoscale silica platelets (NSP) and silver nanoparticles on nanoscale silica platelets (AgNP/NSP), to control Fusarium wilt [caused by f. sp.

Development of a TaqMan Probe-Based Insulated Isothermal Polymerase Chain Reaction (iiPCR) Assay for Detection of Fusarium oxysporum f. sp. cubense Race 4.

This study developed a novel and inexpensive detection method based on a TaqMan probe-based insulated isothermal polymerase chain reaction (iiPCR) method for the rapid detection of Panama disease caused by Fusarium oxysporum f. sp. cubense (Foc) race 4, which is currently among the most serious fungal vascular diseases worldwide.

Thermally pressure-induced partial structural phase transitions in core-shell InSb-SiO2 nanoballs/microballs: characterization, size and interface effect.

Core-shell InSb-SiO(2) nanoballs/microballs were synthesized on a Si substrate by carbonthermal reactions at a temperature of 900 °C. High-resolution transmission microscopy (HRTEM) images revealed that the surfaces of the InSb nanoballs/microballs were covered by amorphous SiO(2) layers. On the basis of our theoretical calculation, the thermal expansion coefficient (TEC) of the InSb crystals is ten times higher than that of the SiO(2) shell.

A study of PKM2, PFK-1, and ANT1 expressions in cervical biopsy tissues in China.

This present study explored the association of Pyruvate Kinase isozyme M2 (PKM2), Phosphofructokinase 1 (PFK-1) and Adenine nucleotide translocator 1 (ANT1) with cervical carcinoma. A case-control method was designed by the collected 95 cervical biopsy samples, which were divided into 30 controls and 60 cases. Cases were subdivided into mild cervical carcinoma (MCC-25), intermediate cervical carcinoma (ICC-20), and severe cervical carcinoma (SCC-20) by method of cervical pathology.

Development of the molecular methods for rapid detection and differentiation of Fusarium oxysporum and F. oxysporum f. sp. niveum in Taiwan.

Fusarium wilt, caused by Fusarium oxysporum (Fo), is one of the most important fungal diseases worldwide. Like other plant pathogens, Fo displays specialized forms in association with its hosts. For example, F.

[Common infantile scrotum or testicle disease diagnosed by high frequency ultrasonoscopy].

Objective: To diagnose and distinguish the swell in the infantile scrotum or testicle by the high frequency ultrasonoscopy so as to find the acoustic imaging characters and clinical application value.

Methods: With the high frequency linear-array probe used, the multi-section and two-side contrast scanning checks were conducted on 46 cases of the infantile scrotum or testicle swell and position with abnormal physical signs. The structures of scrotum, testicle, epididymis and spermatic cord as well as the positions, sizes, forms and interior echoes were observed.

Ring (Y) in two azoospermic men.

We have identified two azoospermic men with r(Y) in 312 infertile men presenting with non-obstructive azoospermia or oligozoospermia. Their karyotypes were 45,X [9]/46,X, r(Y)(p11q11) [11] (case 1), and 46,X,r(Y)(p11q11) (case 2), respectively. In both cases, the Yp breakpoints were located within the pseudoautosomal region.