GhHAM regulates GoPGF-dependent gland development and contributes to broad-spectrum pest resistance in cotton.

Cotton is a globally cultivated crop, producing 87% of the natural fiber used in the global textile industry. The pigment glands, unique to cotton and its relatives, serve as a defense structure against pests and pathogens. However, the molecular mechanism underlying gland formation and the specific role of pigment glands in cotton's pest defense are still not well understood.

[Effects of slow release-fertilizers on yield, nutrient contents and quality of Coptis chinensis].

Objective: To study the effects of four kind of slow-release fertilizers on yield and quality of Coptis chinensis.

Method: One to three years C. chinensis was fertilized with slow-release fertilizers twice in April and in September.

[Study on optimizing extraction methods of Lamiophlomis rotata].

Objective: To optimize different extraction methods of Lamiophlomis rotata Kudo.

Methods: With the content of total flavonoids as assay index, the effect of three extraction methods was compared and the optimal excracting technology was selected by orthogoral test.

Results: The contents of flavonoids and solids by ethanol extraction were more than that by the boiling water extraction and ultrasonic extraction.

Construction of cDNA library of cotton mutant Xiangmian-18 library during gland forming stage.

Gossypol, a secondary metabolite stored in the glands of cotton, protecting cottonseed from consumption of human and monogastric animal. This ability is unique to the tribe Gossypieae. Although the relationship between gossypol and pigment gland has been studied for a long time, the development mechanism of pigment gland has not been investigated at molecular level.

Molecular cloning and expression analysis of a RanBP2 zinc finger protein gene in upland cotton (Gossypium hirsutum L.).

Gossypol is an important resistant substance of Gossypium, and its storage organ is pigment gland. Although, the relationship between gossypol and pigment gland has been studied for a long time, the development mechanism of pigment gland has not been revealed up to now in molecular perspective. On the basis of differentially expressed cDNAs fragments at the stage of the cotton gland development using suppression subtractive hybridization (SSH), the complete cDNA sequence of a novel RanBP2 zinc finger protein (ZFP) gene was cloned by rapid amplification of cDNA ends (RACE) from upland cotton (Gossypium hirsutum L.