Publications by authors named "Ying-Chun Hou"
Short-homology-mediated PCR-based method for gene introduction in the fission yeast Schizosaccharomyces pombe.
Brief Funct Genomics
September 2024
Article Synopsis
- Schizosaccharomyces pombe, or fission yeast, is a useful organism for scientists to study cell functions because it's easy to change its DNA.
- In a recent study, scientists improved the method for adding new genes to S. pombe, which helped them successfully introduce a gene that gives resistance to a specific antibiotic.
- They found that using special techniques and adding certain substances made it easier to insert genes, increasing the chances of success significantly, even with small stretches of DNA.
A CD44 specific peptide developed by phage display for targeting gastric cancer.
Biotechnol Lett
November 2015
Objective: To develop a peptide probe that could be used for gastric cancer detection via binding to CD44 protein with specificity and affinity.
Results: A 12-mer phage peptide library was screened against immobilized CD44 protein. Bound phage counts using ELISA were performed to identify phage clones carrying the most highly selective peptide, which termed RP-1.
Article Synopsis
- ANXA2, an overexpressed protein in tumors, has an unclear role in gastric cancer, prompting research to assess its impact on cancer cell growth.
- The study involved silencing ANXA2 in SGC-7901 gastric cancer cells and measuring various processes like cell proliferation, apoptosis, and motility, revealing that reducing ANXA2 led to significantly decreased cell growth and altered cell structure.
- Overall, the findings suggest that ANXA2 is crucial for maintaining the aggressive characteristics of gastric cancer cells, indicating it could be a potential target for gene therapy.
Aim: To develop an affinity peptide that binds to gastric cancer used for the detection of early gastric cancer.
Methods: A peptide screen was performed by biopanning the PhD-12 phage display library, clearing non-specific binders against tumor-adjacent normal appearing gastric mucosa and obtaining selective binding against freshly harvested gastric cancer tissues. Tumor-targeted binding of selected peptides was confirmed by bound phage counts, enzyme-linked immunosorbent assay, competitive inhibition, fluorescence microscopy and semi-quantitative analysis on immunohistochemistry using different types of cancer tissues.
[Effects of FAK expression level on proliferation and motility of colorectal carcinoma cells].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
September 2009
Aim: To investigate the effect of siRNA targeting FAK gene on proliferation and motility of colorectal cancer.
Methods: Recombinant plasmids that produced siRNAs targeting FAK were designed and cloned, then transfected into Caco-2 cells. The changes of FAK expression levels were examined by RT-PCR and immunocytochemistry.