[Construction of highly effective artifical miRNA targeted to HIV-1 vif and the lentiviral-mediated antiviral research in vitro].

Effective and specific RNA interference (RNAi) elements are essential for the RNAi-based anti-HIV-1 research which has achieved extensive application. vif37 targeted to HIV-1 vi f is a highly effective and conserved RNAi target obtained from the previous study on screening. In this study, we explored the construction of artificial miRNAs to induce RNAi targeted to vif37, which had advantages on inhibition efficiency and flexibility of promoter selection.

Selection of a peptide mimicking neutralization epitope of hepatitis E virus with phage peptide display technology.

Aim: To select the peptide mimicking the neutralization epitope of hepatitis E virus which bound to non-type-specific and conformational monoclonal antibodies (mAbs) 8C11 and 8H3 fromed 7-peptide phage display library, and expressed the peptide recombinant with HBcAg in E.coli, and to observe whether the recombinant HBcAg could still form virus like particle (VLP) and to test the activation of the recombinant polyprotein and chemo-synthesized peptide that was selected by mAb 8H3.

Methods: 8C11 and 8H3 were used to screen for binding peptides through a 7-peptide phage display library.

[Significance of serological markers and virological marker for hepatitis E in rhesus monkey model].

Objective: To evaluate the serological markers and biological marker in the diagnosis of hepatitis E infection in a rhesus monkey model.

Methods: 86 rhesus monkeys had been infected with different doses of HEV. Hence, they were taken sequential blood samples at intervals up to 86 weeks for 4 hepatitis E virus (HEV) specific antibody assays (E2-IgM, E2-IgG, GL-IgG, and YES-IgG), and nucleic acid assay.