Jugular Vein Evans Blue Injection for Blood-Brain Barrier Assessment Following Hemorrhagic Stroke in a Mouse Model.

The blood-brain barrier (BBB) is a selectively permeable membrane that plays a crucial role in protecting the brain from harmful substances. Its disruption is associated with various central nervous system (CNS) disorders, including hemorrhagic stroke. Evans Blue (EB) dye, a protein-binding tracer, is commonly used to assess BBB permeability.

Dietary habits and risk of diabetic kidney disease: a two-sample and multivariate Mendelian randomization study.

Objective: We explored the causal relationship between certain dietary habits and the risk of developing diabetic kidney disease (DKD) using two-sample Mendelian randomization and multivariate Mendelian randomization.

Research Design And Methods: This study is based on pooled data from a genome-wide association study (GWAS) of 83 dietary habits in a European population. We performed a two-sample Mendelian randomization analysis using GAWS data on diabetic nephropathy in a European population.

Real world data on dual BLyS/APRIL inhibition with telitacicept for lupus nephritis.

Background: This study aimed to evaluate the efficacy and safety of telitacicept for the treatment of lupus nephritis (LN) in real-world clinical practice.

Methods: Adult patients with lupus nephritis receiving additional telitacicept at 80/160 mg once per week were recruited, while patients receiving only standard therapy were included as the control group using a 1:1 propensity score matching approach. The primary outcomes were the proportions of patients achieving complete renal response (CRR) and primary efficacy renal response (PERR).

Conjugated Polyelectrolyte-Based Sensor Arrays: from Sensing Mechanisms to Artificial Sensory System Conceptualization.

In the past decades, conjugated polyelectrolytes (CPEs) have become prominent in sensing applications due to their unique properties, including strong and tunable light absorption, high sensitivity, water solubility, and biocompatibility. Inspired by mammalian olfactory and gustatory systems, CPE-based sensor arrays have made significant strides in discriminating structurally similar analytes and complex mixtures for various applications. This review consolidates recent advancements in CPE-based sensor arrays, highlighting rational design, controllable fabrication, and effective data processing methods.

The impact of HBsAg reduction via siRNA treatment on natural and vaccine (BRII-179)-induced HBV-specific humoral and cellular immune responses.

Background And Aims: The impact of HBsAg reduction from small interfering RNA (siRNA) treatments on HBV-specific immunity of CHB participants has not been adequately analyzed in human. We conducted a phase 2a study treating CHB participants with 9 4-weekly doses of HBV-targeted siRNA elebsiran (BRII-835), either alone (n=10) or in combination with a VLP-based therapeutic vaccine (BRII-179) containing Pre-S1, Pre-S2, and S antigens, co-administered with (n=39) or without (n=41) IFNα.

Methods: We analyzed longitudinally for 72 weeks virological, clinical, and immunological parameters including HBsAg, ALT, anti-HBs, the neutralizing activity of representative sera, and frequency and cytokine secretion ability of T cells specific for Pre-S1, Pre-S2, and S both directly ex vivo and after in vitro expansion.

Vertebrae Segmentation with Generative Adversarial Networks for Automatic Cobb Angle Measurement.

Scoliosis is a lateral deformity of the spine, usually diagnosed in patients with a Cobb angle (CA) greater than 10°. Accurate measurements of the CA are necessary for timely intervention and subsequent effective treatment of scoliosis. However, the existing gold standard to calculate the CA requires individual vertebrae pairs to be manually identified, which is a laborious process.

Nomogram-Based Prediction of 3-Month Unfavorable Outcome and Early Neurological Deterioration After Endovascular Thrombectomy in Acute Ischemic Stroke.

Background: Some acute ischemic stroke (AIS) patients due to large-vessel occlusion, who underwent endovascular thrombectomy (EVT), continue to experience unfavorable outcomes. Furthermore, the impact of internal carotid artery (ICA) tortuosity remains uncertain. This study aimed to determine the value of ICA tortuosity and clinical features in predicting 3-month unfavorable outcome and early neurological deterioration (END) after EVT in AIS patients through nomograms.

FastCRL: A Fast Network With Adaptive Fourier Transform and Offset Prediction for Fetal Crown-Rump Length Measurement and Position Estimation in Ultrasound Images.

Fetal crown-rump length (CRL) is one of the most accurate method for estimating gestational age in early pregnancy. Typically, the process of manual CRL measurement by physicians is cumbersome, prone to errors due to fetal position, and susceptible to inter-observer variability. To provide an accurate, real-time, and reliable fetal CRL measurement solution, we propose FastCRL that utilizes key landmarks detection for efficient CRL measurements and fetal position estimation.

An Information-Rich and Highly Wearable Soft Sensor System Based on Displacement Myography for Practical Hand Gesture Interfaces.

Wearable sensors for hand gesture recognition have demonstrated significant potential for creating non-invasive human-machine interfaces. Nonetheless, the trade-off between wearability, practicality and performance constrains their applicability in real-world scenarios. This paper introduces MyoLog, a wearable soft sensor system that utilises forearm muscle deformations for accurate hand gesture recognition.

Enhancing intact allergen protein detection in dairy products: Preventing fragmentation and aggregation with Sinapinic acid salt in MALDI-TOF MS analysis.

The development of efficient detection technology for dairy allergens represents a crucial concern for public health. In this study, a strategy utilizing sinapinic acid salt (SAS) for analyzing allergen proteins through MALDI-TOF MS is presented. SAS has proven to be a superior matrix compared to solid matrices, as it inhibits aggregation and prevents fragmentation, thereby yielding more reliable molecular mass values of intact allergen proteins.

Associations of Systemic Immune-Inflammation Index With Mortality Risk Among Adults in Diabetic Kidney Disease, NHANES 1999-2018.

Background: Immune-Inflammation plays a crucial role in the pathogenesis of diabetic kidney disease (DKD), but the exact assessment of indicators are undefined. Our paper examined the link between Systemic immune-inflammation index (SII) and mortality risk in DKD, and explore the effect of sex disparities on it.

Methods: Data of DKD patients from the National Health and Nutritional Examination Surveys (NHANES) data (1999-2018) were studied and the causes of mortality were identified from NHANES-related files.

OsLC1, a transaldolase, regulates cell patterning and leaf morphology through modulation of secondary metabolism.

Leaf morphogenesis is a crucial process in plants that governs essential physiological functions such as photosynthesis and transpiration. Despite significant advances in understanding leaf development, the mechanism of intricate cellular patterning remains elusive. We characterize the OsLC1 mutant, which displays a curly leaf phenotype alongside reductions in plant height and tiller number, which are indicative of multiple morphological abnormalities.

Macrocycle-based PROTACs selectively degrade cyclophilin A and inhibit HIV-1 and HCV.

Targeting host proteins that are crucial for viral replication offers a promising antiviral strategy. We have designed and characterised antiviral PROteolysis TArgeting Chimeras (PROTACs) targeting the human protein cyclophilin A (CypA), a host cofactor for unrelated viruses including human immunodeficiency virus (HIV) and hepatitis C virus (HCV). The PROTAC warheads are based on fully synthetic macrocycles derived from sanglifehrin A, which are structurally different from the classical Cyp inhibitor, cyclosporine A.

Unveiling the role of risk factors and predictive models in acute type-a aortic dissection surgery: OI downregulation and its association with immune disorders.

Acute type A aortic dissection (ATAAD) represents a critical and life-threatening condition requiring urgent surgical intervention, which is often life-saving. However, postoperative acute lung injury (ALI) has emerged as a prominent complication that significantly impacts patient outcomes and prognosis. This study aims to systematically analyze the risk factors associated with the development of severe ALI following ATAAD surgery, providing insights to improve postoperative management strategies.

Nanoparticle-based drug delivery systems to modulate tumor immune response for glioblastoma treatment.

Glioblastoma (GBM) is a primary central nervous system neoplasm, characterized by a grim prognosis and low survival rates. This unfavorable therapeutic outcome is partially attributed to the inadequate immune infiltration and an immunosuppressive microenvironment, which compromises the effectiveness of conventional radiotherapy and chemotherapy. To this end, precise modulation of cellular dynamics in the immune system has emerged as a promising approach for therapeutic intervention.

Multimodal cross-scale context clusters for classification of mental disorders using functional and structural MRI.

The brain is a complex system with multiple scales and hierarchies, making it challenging to identify abnormalities in individuals with mental disorders. The dynamic segregation and integration of activities across brain regions enable flexible switching between local and global information processing modes. Modeling these scale dynamics within and between brain regions can uncover hidden correlates of brain structure and function in mental disorders.

Catechin-Based Polyphenol Nanoparticles Ameliorated Ferroptosis to Alleviate Brain Injury after Intracerebral Hemorrhage.

Spontaneous intracerebral hemorrhagic stroke (ICH) is a highly aggressive disease, with a high incidence and mortality rate. Iron deposition following ICH leads to oxidative damage and motor dysfunction, significantly impacting the overall quality of life for those affected. Here, a polyphenolic nanomedicine, catechin-based polyphenol nanoparticles surface-modified by thiol-terminated poly(ethylene glycol) (CNPs@PEG), was developed through the oxidative polymerization and self-assembly of catechin, a natural compound in tea.

Selection of biofilm-inhibiting ssDNA aptamers against antibiotic-resistant Edwardsiella tarda by inhibition-SELEX and interaction with their binding proteins.

Biofilms can increase bacterial resistance to antibiotic therapies. Edwardsiella tarda with biofilm is highly resistant to antibacterial treatment, especially for the antibiotic-resistant strain. In this study, we obtained biofilm-inhibiting aptamers against antibiotic-resistant E.

Modular Synthesis of Planar-Chiral Cyclononenes via -Retentive Trapping of π-Allyl-Pd Dipoles.

-cycloalkenes are abundant in bioactive natural products and have been used as powerful tools in chemical biology and drug discovery. However, strategies for the modular synthesis of -cycloalkenes, especially planar-chiral medium-sized ones, with high efficiency and selectivity, still remain elusive. Herein, we report a Pd-catalyzed asymmetric [7 + 2] cyclization strategy to address this challenge.

Transcriptomic analysis of the inhibition mechanisms against by antibacterial aptamer B4.

is a common bacterial pathogen in aquaculture, often leading to visceral white spot disease in large yellow croakers (). Previous studies have found that certain aptamers show an efficient antibacterial effect against this pathogen. In this study, we analyzed the transcriptome of to get insights into the antibacterial and inhibitions mechanisms following exposure to the aptamer B4.

The ignored structure in female fertility: cilia in the fallopian tubes.

Cilia in the fallopian tubes (CFT) play an important role in female infertility, but have not been explored comprehensively. This review reveals the detection techniques for CFT function and morphology, and the related analysis of female infertility and other gynaecological disorders. CFT differentiate from progenitor cells, and develop into primary cilia and motile cilia.

Incidence and related factors of catheter-associated bloodstream infection in neonates: A systematic review and meta-analysis.

Objective: To identify the related factors of neonatal catheter-associated bloodstream infection (CABSI), and provide a basis for constructing a scientific and rational strategy for neonatal CABSI prevention.

Methods: CNKI, Wanfang, PubMed, MEDLINE-Ovid, Web of Science, Embase, CINAHL, and Cochrane Library were searched for publications on neonatal CABSI from the time the database was established until September 30, 2023. The language of publications was not specified.

Mitigating Passivation Layer Damage and Lowering Contact Resistivity of TOPcon Solar Cells Through Low PbO Content Ag Paste.

The development of front-side pastes suitable for devices with high sheet resistance such as tunnel oxide passivated contact (TOPCon), is of great significance but remains a considerable challenge. The optimization of the Ag-Si contact interface is crucial for enhancing contact and improving the efficiency of these devices. This work investigates the front-side Ag pastes with low Al content (<2 wt.