Metric method for sex determination based on the 12th thoracic vertebra in contemporary north-easterners in China.

Many studies have shown that discriminant function equations, used in the determination of sex from skeletons, are population specific. The aim of the present research was to develop discriminant function equations for sex determination using the 12th thoracic vertebra (T12) in a contemporary northeast Chinese sample and to investigate whether the differences of the T12 between males and females consist more in shape than in size. Thirty linear measurements were obtained from 141 three-dimensional reconstructed T12 models (78 males and 63 females), and then 112 ratios were calculated by using the thirty linear measurements.

Effects of GM-CSF, IL-3, and GM-CSF/IL-3 fusion protein on apoptosis of human myeloid leukemic cell line Tf-1 induced by irradiation.

Aim: To observe the effects of three cytokines on the apoptosis of Tf-1 cells induced by gamma irradiation and investigate the relationship between apoptosis and caspase-3 activity.

Methods: Different cytokines GM-CSF, IL-3 and GM-CS/IL-3 fusion protein were added into the irradiated Tf-1 cells. MTT assay, morphology, flow cytometry, and DNA fragmentation assay were used to observe the effects of cytokines on apoptosis.

[In vitro and in vivo functional evaluation of anti-human bladder tumor human-mouse chimeric antibody ch-BDI].

Objective: To evaluate the in vitro and in vivo function of anti-human bladder tumor human-mouse chimeric antibody ch-BDI and its future clinical application.

Methods: With ch-BDI in high-expression cell-line medium, affinity chromatography was used for the purification. Labeled with (99m)Tc through reduction method, its immunoreactive fraction and association constant were measured.