Prevalence and Risk Factors of Multidrug-Resistant Infection in Clinical Dogs and Cats - China, 2018-2021.

What Is Already Known About This Topic?: spp., while naturally occurring as commensal bacteria in the gastrointestinal tract of animals and humans, have emerged as significant opportunistic pathogens in healthcare settings.

What Is Added By This Report?: A comprehensive surveillance study revealed enterococci in 14.

High paracrine activity of hADSCs cartilage microtissues inhibits extracellular matrix degradation and promotes cartilage regeneration.

Due to its unique structure, articular cartilage has limited self-repair capacity. Microtissues are tiny tissue clusters that can mimic the function of target organs or tissues. Using cells alone for microtissue construction often results in the formation of necrotic cores.

Boron contributes to enhance antimony tolerance in rice (Oryza sativa L.) by activating antioxidant system, modifying the cell wall component and promoting cell wall deposition of Sb.

Boron (B) is essential for plant growth and helps mitigate metal toxicity in various crop plants. However, the potential role and underlying mechanisms of B in alleviating antimony (Sb) toxicity in rice remain unexplored. In this study, we investigated the effects of H₃BO₃ supplementation (30, 50, and 75 μM) on morphological growth, physiological and biochemical traits, Sb content, and the subcellular distribution of Sb in rice plants under 100 μM Sb stress during the seedling stage in a hydroponic system.

Gut-liver translocation of pathogen Klebsiella pneumoniae promotes hepatocellular carcinoma in mice.

Hepatocellular carcinoma (HCC) is accompanied by an altered gut microbiota but whether the latter contributes to carcinogenesis is unclear. Here we show that faecal microbiota transplantation (FMT) using stool samples from patients with HCC spontaneously initiate liver inflammation, fibrosis and dysplasia in wild-type mice, and accelerate disease progression in a mouse model of HCC. We find that HCC-FMT results in gut barrier injury and translocation of live bacteria to the liver.

Two birds with one stone: Alleviating copper toxicity and inhibiting its upward transport in non-host rice (Oryza sativa L.) by inoculation of Cu-resistant endophytes from the hyperaccumulator Commelina communis.

Endophytic bacteria derived from metal hyperaccumulators have demonstrated potential for improving copper (Cu) remediation in host plants; however, their potential application in non-host crops remains unclear. In this study, endophytic bacteria isolated from Commelina communis growing in mining areas and their mitigation effects on Cu toxicity in non-host rice were comprehensively evaluated. Among the isolated endophytes, Bacillus sp.

Triplet Network for One-Shot Raman Spectrum Recognition.

Raman spectroscopy is widely used for material detection due to its specificity, but its application to spectral recognition often faces limitations due to insufficient training data, unlike fields such as image recognition. Traditional machine learning or basic neural networks are commonly used, but they have limited ability to achieve high precision. We have proposed a novel approach that combines the Triplet network (TN) and K-nearest neighbor (KNN) techniques to address this issue.

An OPRM1-SNAP-tag/CMC method to directly identify drug components in sewage.

The scourge of drug addiction and abuse poses a significant challenge to society. Opioid drugs acting on μ-opioid receptor (OPRM1) make it one of the pivotal targets for drug addiction. In the past decade, sewage analysis has become a prevalent method of drug monitoring.

Bio-organic substitution in tobacco (Nicotiana tabacum L) cultivation: Optimum strategy to lower carbon footprint and boost net ecosystem economic benefit.

The partial substitution of organic manure for chemical nitrogen fertilizers, known as organic substitution, is widely regarded as a cleaner and more sustainable production strategy. However, few studies have quantified greenhouse gas emissions, product income and net ecosystem economic benefit (NEEB) using a life cycle assessment (LCA) approach, particularly for typical tobacco (Nicotiana tabacum L.) production.

Multi-pesticide residue screening, identification, and quantification analysis in various fruits and vegetables by UHPLC-Q Exactive HRMS.

A general strategy for qualitative screening and quantitative analysis of 403 pesticides in various fruits and vegetables was developed using ultrahigh-performance liquid chromatography (UHPLC) in conjunction with a Thermo Q Exactive Focus high-resolution mass spectrometer, relying on an executable compound database comprised of the exacted mass of precursor ions, retention times, and fragment ions. Taking advantage of the powerful separation capacity of UHPLC, an Orbitrap analyzer with high sensitivity in full scan mode and elevated mass resolution of product ions in the MS/MS mode, eight pairs of isomers and fifty-seven groups of isobaric compounds were selectively identified. The method was then systematically assessed and validated for eight fruits and vegetables, in terms of screening detection limit (SDL), matrix effects, recovery, and precision over 400 pesticides.

Efficient removal of Sb(III) from wastewater using selenium nanoparticles synthesized by Psidium guajava plant extract.

Antimony (Sb) pollution in aquatic ecosystems has emerged as a critical environmental issue on a global scale, emphasizing the urgent need for cost-effective and user-friendly technologies to remove Sb compounds from water sources. In this study, a novel adsorbent, selenium nanoparticles (SeNPs), was synthesized using the aqueous extract of Psidium guajava L. leaves (AEP) for the purpose of eliminating Sb(III) from aqueous solutions.

Biosynthetic selenium nanoparticles (Bio-SeNPs) mitigate the toxicity of antimony (Sb) in rice (Oryza sativa L.) by limiting Sb uptake, improving antioxidant defense system and regulating stress-related gene expression.

Nanotechnology offers a promising and innovative approach to mitigate biotic and abiotic stress in crop production. In this study, the beneficial role and potential detoxification mechanism of biogenic selenium nanoparticles (Bio-SeNPs) prepared from Psidium guajava extracts in alleviating antimony (Sb) toxicity in rice seedlings (Oryza sativa L.) were investigated.

TMEM120B strengthens breast cancer cell stemness and accelerates chemotherapy resistance via β1-integrin/FAK-TAZ-mTOR signaling axis by binding to MYH9.

Background: Breast cancer stem cell (CSC) expansion results in tumor progression and chemoresistance; however, the modulation of CSC pluripotency remains unexplored. Transmembrane protein 120B (TMEM120B) is a newly discovered protein expressed in human tissues, especially in malignant tissues; however, its role in CSC expansion has not been studied. This study aimed to determine the role of TMEM120B in transcriptional coactivator with PDZ-binding motif (TAZ)-mediated CSC expansion and chemotherapy resistance.

The role of an Sb-oxidizing bacterium in modulating antimony speciation and iron plaque formation to reduce the accumulation and toxicity of Sb in rice (Oryza sativa L.).

Microbial antimony (Sb) oxidation in the root rhizosphere and the formation of iron plaque (IP) on the root surface are considered as two separate strategies to mitigate Sb(III) phytotoxicity. Here, the effect of an Sb-oxidizing bacterium Bacillus sp. S3 on IP characteristics of rice exposed to Sb(III) and its alleviating effects on plant growth were investigated.

Selenium bio-nanocomposite based on extracellular polymeric substances (EPS): Synthesis, characterization and application in alleviating cadmium toxicity in rice (Oryza sativa L.).

Selenium nanoparticles (SeNPs) have gained significant attention owing to their favorable bioavailability and low toxicity, making them widely applications in the fields of medicine, food and agriculture. In this study, bacterial extracellular polymeric substances (EPS) were used as a novel stabilizer and capping agent to prepare dispersed SeNPs. Results show that EPS-SeNPs presented negative potential (-38 mV), spherical morphologies with average particle size about 100-200 nm and kept stable at room temperature for a long time.

Curcumin ameliorates pulmonary fibrosis progression by inhibiting the fibrotic process of lung fibroblasts.

The objective of this study was to analyze the effect of curcumin (Cur) on pulmonary fibrosis (PF), so as to provide new clinical evidence for future PF treatment. To achieve these goals, the researchers set up bought human lung fibroblasts MRC-5 as a control group without treatment, a model group for PF cell modeling, and an intervention group for Cur intervention after PF modeling. Cell proliferation capacity and cellular TGF-β1, α-SMA, Collagen I, Collagen III, Bax, N-cadherin and E-cadherin protein expression were determined.

ZNF500 abolishes breast cancer proliferation and sensitizes chemotherapy by stabilizing P53 via competing with MDM2.

Zinc finger protein 500 (ZNF500) has an unknown expression pattern and biological function in human tissues. Our study revealed that the ZNF500 mRNA and protein levels were higher in breast cancer tissues than those in their normal counterparts. However, ZNF500 expression was negatively correlated with advanced TNM stage (p = 0.

Metabolomics acts as a powerful tool for comprehensively evaluating vaccines approved under emergency: a CoronaVac retrospective study.

Introduction: To control the COVID-19 pandemic, great efforts have been made to realize herd immunity by vaccination since 2020. Unfortunately, most of the vaccines against COVID-19 were approved in emergency without a full-cycle and comprehensive evaluation process as recommended to the previous vaccines. Metabolome has a close tie with the phenotype and can sensitively reflect the responses to stimuli, rendering metabolomic analysis have the potential to appraise and monitor vaccine effects authentically.

Comprehensive analysis of the expression and prognosis for RAI2: A promising biomarker in breast cancer.

Introduction: Retinoic acid-induced 2 (RAI2) was initially related to cell differentiation and induced by retinoic acid. RAI2 has been identified as an emerging tumor suppressor in breast cancer and colorectal cancer.

Methods: In this study, we performed systematic analyses of RAI2 in breast cancer.

Biochar amendments and climate warming affected nitrification associated NO and NO production in a vegetable field.

Soil nitrification driven by ammonia-oxidizing microorganisms is the most important source of nitrous oxide (NO) and nitric oxide (NO). Biochar amendment has been proposed as the most promising measure for combating climate warming; both have the potential to regulate the soil nitrification process. However, the comprehensive impacts of different aged biochars and warming combinations on soil nitrification-related NO and NO production are not well understood.

The prospect of combination therapies with the third-generation EGFR-TKIs to overcome the resistance in NSCLC.

Epidermal growth factor receptor-tyrosine kinase inhibitors (EGFR-TKIs) bring significant benefits to non-small cell lung cancer patients with EGFR mutations, which represent a breakthrough in lung cancer therapy. However, patients will ultimately develop the acquired resistance to the first- or second-generation EGFR-TKIs after a period of treatment, and EGFR T790M mutation is the most common resistant mechanism. The third-generation EGFR-TKIs target T790M mutation and show potent anti-tumor efficacy, especially in central neural system response.

A genomic instability-related lncRNA model for predicting prognosis and immune checkpoint inhibitor efficacy in breast cancer.

Breast cancer has overtaken lung cancer as the most frequently diagnosed cancer type and is the leading cause of death for women worldwide. It has been demonstrated in published studies that long non-coding RNAs (lncRNAs) involved in genomic stability are closely associated with the progression of breast cancer, and remarkably, genomic stability has been shown to predict the response to immune checkpoint inhibitors (ICIs) in cancer therapy, especially colorectal cancer. Therefore, it is of interest to explore somatic mutator-derived lncRNAs in predicting the prognosis and ICI efficacy in breast cancer patients.

Detection and Significance of Cell-Free DNA Mutation in Pleural Effusion in Patients with Advanced NSCLC.

Objective: To detect EGFR/KRAS genes in pleural effusion cell-free DNA in patients with advanced non-small-cell lung cancer (NSCLC) and to explore the clinical significance of EGFR/KRAS mutation status in pleural effusion.

Methods: A retrospective collection was performed on the specimens of pleural effusion and matched tissues from 50 patients with advanced NSCLC admitted to the hospital between January 2019 and January 2021. DNA mutation status of EGFR/KRAS in different specimens was detected and compared by pyrosequencing.

Empirical antibiotic treatment strategies for community-acquired pneumonia: a network meta-analysis.

Objectives: This network meta-analysis aimed to compare the efficacy and safety of fluoroquinolone (FQ) monotherapy, β-lactam (BL) monotherapy and β-lactam/macrolide (BL-M) combination therapy in hospitalized patients with community-acquired pneumonia (CAP).

Methods: Pubmed, Embase and the Cochrane Library were searched for randomized controlled trials (RCTs) comparing FQ monotherapy, BL monotherapy and BL-M combination therapy up to July 2021. The outcomes of interest included all-cause mortality, clinical success, microbiological success and drug-related adverse events.

Electrochemical Difunctionalization of Terminal Alkynes: Access to 1,4-Dicarbonyl Compounds.

1,4-Dicarbonyl compounds are versatile scaffolds for the heterocycle synthesis, including the Paal-Knorr reaction. Herein, a feasible electrosynthesis method to access 1,4-dicarbonyl compounds has been developed from simple alkynes and 1,3-dicarbonyl compounds. When the undivided cell is combined with the constant current mode, aryl alkynes containing numerous medicinal motifs with 1,3-dicarbonyl esters or ketones react smoothly.