Publications by authors named "Yindong Zhao"
Antigen recognition by T cells is degenerate both at the MHC and the TCR level. In this study, we analyzed the cross-reactivity of a human HIV-1 gag p24-specific CD4(+) T cell clone obtained from an HIV-1-seronegative donor using a positional scanning synthetic combinatorial peptide library (PS-SCL)-based biometrical analysis. A number of decapeptides able to activate the HIV-1 gag-specific clone were identified and shown to correspond to sequences found in other human pathogens.
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- A novel method was used to find tumor antigens that CD8(+) T cells can recognize by screening a vast library of synthetic peptides.
- The research involved testing a library of over 300 billion nonapeptides in a cytotoxicity assay to identify which peptides were recognized by melanoma-reactive CTLs.
- A mathematical analysis of the screening results led to the identification of a native antigenic peptide, showing the effectiveness of this approach in predicting CTL ligands.