Directed evolution engineering to improve activity of glucose dehydrogenase by increasing pocket hydrophobicity.

Glucose dehydrogenase (GDH) is a NAD(P) dependent oxidoreductase, which is useful in glucose determination kits, glucose biosensors, cofactor regeneration, and biofuel cells. However, the low efficiency of the catalysis hinders the use of GDH in industrial applications. In this study, an analysis of interactions between eight GDH mutants and NADP is powered by AlphaFold2 and Discovery Studio 3.

Transcriptome Analysis of for Improving Bacitracin Production.

This study aims to find the targets that may influence the production of bacitracin based on RNA sequencing in . Transcriptional profiling revealed that (i) the expression of the gene, encoding a type II thioesterase (TEII), was positively correlated with bacitracin production and (ii) the oxygen uptake exhibited significant influence on precursor synthesis. The verified experiments showed that the overexpression of TEII with an endogenous promoter increased the bacitracin A titer by 37.

Combining Precursor-Directed Engineering with Modular Designing: An Effective Strategy for De Novo Biosynthesis of l-DOPA in .

3-Hydroxy-l-tyrosine (l-DOPA) is a promising drug for treating Parkinson's disease. Tyrosine hydroxylase catalyzes the microbial synthesis of l-DOPA, which is hindered by the efficiency of catalysis, the supply of cofactor tetrahydrobiopterin, and the regulation of the pathway. In this study, the modular engineering strategy in was identified to effectively enhance l-DOPA production.

Unraveling the specific regulation of the shikimate pathway for tyrosine accumulation in Bacillus licheniformis.

L-Tyrosine serves as a common precursor for multiple valuable secondary metabolites. Synthesis of this aromatic amino acid in Bacillus licheniformis occurs via the shikimate pathway, but the underlying mechanisms involving metabolic regulation remain unclear. In this work, improved L-tyrosine accumulation was achieved in B.