Drying-wetting cycle enhances stress resistance of Escherichia coli O157:H7 in a model soil.

Outbreaks of Escherichia coli (E. coli) O157:H7 in farms are often triggered by heavy rains and flooding. Most cells die with the decreasing of soil moisture, while few cells enter a dormant state and then resuscitate after rewetting.

Multi-scale simulations of the mechanical behaviors of the W-Cu joint interface with a diffusion layer.

Context: At the interface of W-Cu after direct jointing, diffusion layers with a thickness of approximately 22 nm are present but often overlooked in simulations of mechanical properties. In this study, an interface model with a W-Cu diffusion layer is developed using molecular dynamics (MD). The effects of the diffusion layers on the elastic-plastic behaviors, dissipation mechanisms, and fracture properties of the interface are analyzed under mode-I (perpendicular to the interface) and mode-II (parallel to the interface).

Crop rotation stage has a greater effect than fertilisation on soil microbiome assembly and enzymatic stoichiometry.

Agronomic practises, such as fertilisation and crop rotation, affect soil microbial communities and functions. However, limited information is available regarding the relative importance of fertilisation and crop rotation stages in determining the soil microbiome and assembly processes. In addition, insights into the connections between the soil microbiome and enzymatic stoichiometry are scarce.

The inhibition effect of tea polyphenols on soil nitrification is greater than denitrification in tea garden soil.

Tea polyphenols are the most widely distributed class of secondary metabolites (Camellia sinensis) and account for a considerable proportion of the pruning residues of tea. A large amount of tea polyphenols have fallen down over soil with prunning residues every year. However, the effect of tea polyphenols on soil nitrogen cycle, especially the denitrification process and its related microbial communities, remains unclear.

Effects of glucose on the uptake and metabolism of glycine in pakchoi (Brassica chinensis L.) exposed to various nitrogen sources.

Background: Plants can absorb amino acids as a nitrogen (N) source, and glucose is an important part of root rhizodeposition and the soil sugar pool, which participates in the regulation of plant growth and uptake. In pakchoi, the effect of glucose concentration on the glycine N uptake from a nutrient mixture composed of glycine, ammonium, and nitrate, or from a single N solution of glycine alone was studied using specific substrate N-labeling and N-gas chromatography mass spectrometry.

Results: The optimal glucose concentration for plant growth was 4.

Hexavalent chromium stress enhances the uptake of nitrate but reduces the uptake of ammonium and glycine in pak choi (Brassica chinensis L.).

Chromium (Cr) pollution affects plant growth and biochemical processes, so, the relative uptake of glycine, nitrate, and ammonium by pak choi (Brassica chinensis) seedlings in treatments with 0mgL and 10mgL Cr (VI) were detected by substrate-specific N-labelling in a sterile environment. The short-term uptake of N-labelled sources and N-enriched amino acids were detected by gas chromatography mass spectrometry to explore the mechanism by which Cr stress affects glycine uptake and metabolism, which showing that Cr stress hindered the uptake of ammonium and glycine but increased significantly the uptake of nitrate. Cr stress did not decrease the active or passive uptake of glycine, but it inhibited the conversion of glycine to serine in pak choi roots, indicating that the metabolism of glycine to serine in roots, rather than the root uptake, was the limiting step in glycine contribution to total N uptake in pak choi.

Broadband Light-Harvesting Molecular Triads with High FRET Efficiency Based on the Coumarin-Rhodamine-BODIPY Platform.

Broadband capturing and FRET-based light-harvesting molecular triads, CRBs, based on the coumarin-rhodamine-BODIPY platform were rationally designed and synthesized. The absorption band of CRBs starts from blue-green to yellow-orange regions (330-610 nm), covering the strong radiation scope of sunlight. The peripheral coumarin and BODIPY chromophore energy could transfer to the central acceptor rhodamine by a one-step direct way.

A native-chemical-ligation-mechanism-based ratiometric fluorescent probe for aminothiols.

Thiol-containing amino acids (aminothiols) such as cysteine (Cys) and homocysteine (Hcy) play a key role in various biological processes including maintaining the homeostasis of biological thiols. However, abnormal levels of aminothiols are associated with a variety of diseases. The native chemical ligation (NCL) reaction has attracted great attention in the fields of chemistry and biology.

Fluorescent detection of hypochlorous acid from turn-on to FRET-based ratiometry by a HOCl-mediated cyclization reaction.

Hypochlorous acid (HOCl), a reactive oxygen species (ROS), plays a significant biological role in living systems. However, abnormal levels of HOCl are implicated in many inflammation-associated diseases. Therefore, the detection of HOCl is of great importance.

Development of FRET-based ratiometric fluorescent Cu2+ chemodosimeters and the applications for living cell imaging.

Coumarin-rhodamine-based compounds 1a,b were rationally designed and synthesized as novel FRET ratiometric fluorescent chemodosimeters. Ratiometric chemodosimeters 1a,b exhibit several favorable features, including a large variation in the emission ratio, well-resolved emission peaks, high sensitivity, high selectivity, low cytotoxicity, and good cell membrane permeability. Importantly, these excellent attributes enable us to demonstrate ratiometric imaging of Cu(2+) in living cells by using these novel ratiometric fluorescent chemodosimeters.

Single fluorescent probe responds to H2O2, NO, and H2O2/NO with three different sets of fluorescence signals.

Hydrogen peroxide (H(2)O(2)) acts as a signaling molecule in a wide variety of signaling transduction processes and an oxidative stress marker in aging and disease. However, excessive H(2)O(2) production is implicated with various diseases. Nitric oxide (NO) serves as a secondary messenger inducing vascular smooth muscle relaxation.

Development of a ratiometric fluorescent sensor for ratiometric imaging of endogenously produced nitric oxide in macrophage cells.

We described the development of Cou-Rho-NO as the first small-molecule suitable for ratiometric fluorescent imaging of endogenously produced nitric oxide in macrophage cells.