OTUD6A promotes prostate tumorigenesis via deubiquitinating Brg1 and AR.

Ovarian tumor (OTU) subfamily deubiquitinases are involved in various cellular processes, such as inflammation, ferroptosis and tumorigenesis; however, their pathological roles in prostate cancer (PCa) remain largely unexplored. In this study, we observed that several OTU members displayed genomic amplification in PCa, among which ovarian tumor deubiquitinase 6A (OTUD6A) amplified in the top around 15-20%. Further clinical investigation showed that the OTUD6A protein was highly expressed in prostate tumors, and increased OTUD6A expression correlated with a higher biochemical recurrence risk after prostatectomy.

Effects of different mulching and fertilization on phosphorus transformation in upland farmland.

In the present study, the impact of different soil surface mulching, fertilization on phosphorus mineralization and bio-availability of spring maize at various growth stages and soil layers (0-20 and 20-40 cm soil layer) were evaluated. The results indicated that the contents of total P and Olsen-Phosphorus (Olsen-P) in the soils of 0-20 cm soil layer were significantly higher than those in the 20-40 cm soil layer at different stages. The addition of organic fertilizer significantly increased the soil total P and Olsen-P content in the 0-20 cm soil layer.

Cloning and molecular characterization of rutin degrading enzyme from tartary buckwheat (Fagopyrum tataricum Gaertn.).

Rutin and quercetin, abundant in tartary buckwheat, have physiological and pharmacological functions and play roles in abiotic stress tolerance in plant. Rutin degrading enzymes (RDE) are the key enzymes for rutin metabolism. However, the RDE coding sequence information has not been available.

[Recombinant expression of truncated exonuclease Ⅷ and its application in in vitro DNA recombination].

Exonuclease Ⅷ (Exo Ⅷ), an ATP-independent dsDNA 5'-3' exonuclease, is a candidate protein with great application value for in vitro DNA recombination. However, the application of Exo Ⅷ in DNA recombination in vitro has not been reported. In this study, the recombinant expression vector of the truncated Exo Ⅷ (tExo Ⅷ) with the full exonuclease activity was built and used to achieve the overexpression of tExo Ⅷ in Escherichia coli.