[Construction of vector tandem expressing rat ventricular myocyte Kir2.1 shRNA and its effect in vitro].

Objective: To construct a recombinant vector that expresses 5 shRNAs targeting on the rat ventricular myocyte Kir2.1 gene in tandem and its effect in vitro

Methods: Ventricular myocytes were collected from newborn Wistar rats and cultured. Five sites targeting on the rat Kir2.

[Clinical analysis of 24 supracardiac total anomalous pulmonary venous connection treated with surgery].

Objective: To review the surgical treatment of supracardiac type of total anomalous pulmonary venous connection (TAPVC).

Methods: Twenty-four patients with supracardiac total anomalous pulmonary venous connection underwent surgical correction from July 1995 to June 2004. There were 11 males, 13 females.