The study aimed to explore how antisense MMP-2 affects vascular endothelial cells derived from human hemangiomas by using Ad-aMMP-2 for transfection.
Three experimental groups were tested (M199, Ad-GFP, and Ad-aMMP-2), and techniques like RT-PCR and Western Blotting were used to measure MMP-2 levels.
Results showed that transfection with Ad-aMMP-2 significantly reduced MMP-2 mRNA and protein levels in the endothelial cells compared to the other groups.