The assessment of the quality of reporting of systematic reviews/meta-analyses in diagnostic tests published by authors in China.

Background: The quality of reporting in systematic reviews (SRs)/meta-analyses (MAs) of diagnostic tests published by authors in China has not been evaluated. The aims of present study are to evaluate the quality of reporting in diagnostic SRs/MAs using the PRISMA statement and determine the changes in the quality of reporting over time.

Methods: According to the inclusion and exclusion criteria, we searched five databases including Chinese Biomedical Literature Database, PubMed, EMBASE, the Cochrane Library, and Web of knowledge, to identify SRs/MAs on diagnostic tests.

Proteomic analysis of the effect of diclazuril on second-generation merozoites of Eimeria tenella.

Diclazuril has long been used as an effective benzeneacetonitrile anticoccidial for the control of Eimeria tenella that causes coccidiosis. However, the molecular mechanism underlying the anticoccidial effects of diclazuril remains elusive. In this study, a proteomic analysis of the effect of diclazuril on second-generation merozoites of E.

Information derived from cluster ions from DNA-modified gold nanoparticles under laser desorption/ionization: analysis of coverage, structure, and single-nucleotide polymorphism.

In this study, we employed laser desorption/ionization mass spectrometry (LDI-MS) to explore the interactions between thiolated DNA (HS-DNA) and gold nanoparticles (Au NPs). Under nanosecond-pulsed laser irradiation (Nd:YAG, 355 nm), the efficiency of Au cluster ion formation from the Au NPs decreased in the presence of HS-DNA. At the optimal laser power density (2.

Pulsed-laser desorption/ionization of clusters from biofunctional gold nanoparticles: implications for protein detections.

In this paper, we describe a pulsed-laser desorption/ionization mass spectrometry (LDI-MS) approach for the detection of proteins with femtomolar sensitivity through the analysis of gold (Au) clusters desorbed from aptamer-modified gold nanoparticles (Apt-AuNPs) on a nitrocellulose membrane (NCM). After the target protein (thrombin) was selectively captured by the surface-bound 29-mer thrombin-binding aptamer (TBA(29)), the thrombin/TBA(29)-AuNP complexes were concentrated and deposited onto the NCM to form a highly efficient background-free surface-assisted LDI substrate. Under pulsed laser irradiation (355 nm), the binding of thrombin decreased the desorption and/or ionization efficiencies of the Au atoms from the AuNP surfaces.