Bone regeneration by nanohydroxyapatite/chitosan/poly(lactide-co-glycolide) scaffolds seeded with human umbilical cord mesenchymal stem cells in the calvarial defects of the nude mice.

In the preliminary study, we have found an excellent osteogenic property of nanohydroxyapatite/chitosan/poly(lactide-co-glycolide) (nHA/CS/PLGA) scaffolds seeded with human umbilical cord mesenchymal stem cells (hUCMSCs) in vitro and subcutaneously in the nude mice. The aim of this study was to further evaluate the osteogenic capacity of nHA/CS/PLGA scaffolds seeded with hUCMSCs in the calvarial defects of the nude mice. Totally 108 nude mice were included and divided into 6 groups: PLGA scaffolds + hUCMSCs; nHA/PLGA scaffolds + hUCMSCs; CS/PLGA scaffolds + hUCMSCs; nHA/CS/PLGA scaffolds + hUCMSCs; nHA/CS/PLGA scaffolds without seeding; the control group (no scaffolds) (n = 18).

Xiaotan Sanjie decoction attenuates tumor angiogenesis by manipulating Notch-1-regulated proliferation of gastric cancer stem-like cells.

Aim: To determine the underlying mechanisms of action and influence of Xiaotan Sanjie (XTSJ) decoction on gastric cancer stem-like cells (GCSCs).

Methods: The gastric cancer cell line MKN-45 line was selected and sorted by FACS using the cancer stem cell marker CD44; the stemness of these cells was checked in our previous study. In an in vitro study, the expression of Notch-1, Hes1, Vascular endothelial growth factor (VEGF), and Ki-67 in both CD44-positive gastric cancer stem-like cells (GCSCs) and CD44-negative cells was measured by Western blot.

Xiaotan Tongfu granules contribute to the prevention of stress ulcers.

Aim: To investigate the efficacy and potential mechanism of Xiaotan Tongfu granules (XTTF) in stress ulcers.

Methods: One hundred sixty rats were randomly divided into 4 groups (n = 10) as follows: the model group (MP group), the control group (CP group), the ranitidine group (RP group) and the XTTF granule group (XP group). Rats in the MP group received no drugs, rats in the CP group received 0.

Evaluation of in vitro and in vivo osteogenic differentiation of nano-hydroxyapatite/chitosan/poly(lactide-co-glycolide) scaffolds with human umbilical cord mesenchymal stem cells.

We aimed to evaluate the feasibility of the application of the nano-hydroxyapatite/chitosan/poly(lactide-co-glycolide) (nHA/CS/PLGA) scaffold seeded with human umbilical cord mesenchymal stem cells (hUCMSCs) in bone tissue engineering. We prepared the nHA/CS/PLGA, nHA/PLGA, CS/PLGA, and PLGA scaffolds, and tested their mechanical strength. We analyzed the surface antigen markers of hUCMSCs to determine their capability to differentiate into osteoblasts, chondrocytes, and adipocytes.

[Construction and identification of BIRC5 shRNA lentiviral expression vector].

Aim: Design and synthesis complementary DNA sequences of 3 pairs of short hairpin structure and a pair of negative control sequence by RNA interference technique and construct and identify a lentiviral interference vector with human BIRC5 gene as target gene.

Methods: The designed and synthesised Single-Stranded primer were annealed to Double-Stranded oligo sequences and subcloned into linear pMAGic lentiviral plasmid vector digested by enzyme Age I and EcoR I. Screening positive clone after transformed into DH5α competent cells and identified by PCR amplification and DNA sequencing.

[Killing effect of cytotoxicty T lymphocyte primed by two different methods preparation of dendritic cells pulsed with antigen on Tca8113 cells in vitro].

Objective: To study the feasibility of dendritic cells (DCs) vaccine on the therapy of tongue carcinoma and find the better way of antigen load.

Methods: The antigen peptides of Tca8113 cells were obtained by acid eluted technique and repetitive freeze thaw method. Separating T cell and inducing dendritic cells were obtained from human peripheral blood monocyte.

[Diagnosis and surgical approach of parapharyngeal space neoplasms].

Purpose: The aim of this study is to investigate the diagnosis and reasonable surgical approach for parapharyngeal space neoplasms.

Methods: A retrospective study was carried out from the data of 24 patients, who suffered from paralaryngeal space neoplasms and underwent surgical treatment from 1998 to 2006. The follow- up was carried out from 1 to 10 years.

[Arthroscopic investigation of the status of temporomandibular joint in patients with temporomandibular joint pain].

Objective: To investigate the status of the temporomandibular joint (TMJ) in patients with complaints of joint pain for improving clinical therapy.

Methods: Twenty three joints in twenty patients who complained of TMJ pain were examined radiographically and arthroscopically.

Results: There were at least one and more to six pathological changes could be found arthroscopically in the 23 temporomandibular joints, which were different in some respects with radiographic findings.