Single-cell transcriptomic landscape of the sheep rumen provides insights into physiological programming development and adaptation of digestive strategies.

As an important evolutionary innovation and unique organ, the rumen has played a crucial role in ruminant adaptation to complex ecological environments. However, the cellular basis of its complex morphology and function remains largely unknown. In this study, we identified eight major cell types from seven representative prenatal and postnatal rumen samples using ~56 600 single-cell transcriptomes.

A high-efficiency MUF method benefits postoperative hemodynamic stability and oxygen delivery in neonates with transposition of great arteries.

Objective: To minimize the postoperative hemodynamic changes in neonates with transposition of great arteries (TGA) by modifying the circuit and efficiency of traditional modified ultrafiltration (MUF).

Methods: Ninety-one patients (<5 kg) underwent arterial switch operations, which were randomized to a traditional MUF (tMUF) group (N = 38) or a modified MUF (mMUF) group (N = 53). MUF of both the groups lasted for 8 to 12 minutes, during which the near-infrared spectroscopy was used to monitor cerebral tissue oxygenation parameters.

Effects of Acarbose Addition on Ruminal Bacterial Microbiota, Lipopolysaccharide Levels and Fermentation Characteristics In vitro.

This study investigated the effects of acarbose addition on changes in ruminal fermentation characteristics and the composition of the ruminal bacterial community in vitro using batch cultures. Rumen fluid was collected from the rumens of three cannulated Holstein cattle fed forage ad libitum that was supplemented with 6 kg of concentrate. The batch cultures consisted of 8 mL of strained rumen fluid in 40 mL of an anaerobic buffer containing 0.

Two new acridone alkaloids from the branch of Atalantia buxifolia and their biological activity.

Two new acridone alkaloids, 3-methoxy-1,4,5-trihydroxy-10-methylacridone (1) and 2,3-dimethoxy-1,4,5-trihydroxy-10-methylacridone (2), were isolated from the ethanol extract of the branch of Atalantia buxifolia. Their structures were elucidated by spectroscopic methods including 1D and 2D NMR. Compounds 1 and 2 exhibited significant antibacterial activity against Staphylococcus aureus and weak inhibitory effect on acetylcholinesterase.