[Complete genome sequence analysis of Japanese encephalitis virus newly isolated in China].

Objective: To study the complete genome sequence of Japanese encephalitis virus (JEV) strain XJ69 isolated in ZheJiang province and explore its evolution.

Methods: Overlapping primers were designed according to the full-length genomes from GenBank. RT-PCR was used to amplify the fragments and RT-PCR products were cloned T vector, sequenced and analyzed.

[Development and evaluation of TaqMan-based one-step reverse transcription-polymerase chain reaction assay for the detection of Japanese encephalitis virus].

Objective: To establish a TaqMan based real-time reverse transcription-polymerase chain reaction (RT-PCR) assay for the detection of Japanese encephalitis virus.

Methods: The gene sequences of Japanese encephalitis virus downloaded from the GenBank was aligned, using the biologic software. Specific primers and probes were designed in the conserved region of the C gene for Japanese encephalitis virus.

[The distributions of mosquito vectors carrying Japanese encephalitis virus in Zhejiang province].

Objective: To investigate the natural infection and genotype of Japanese encephalitis virus in mosquitoes and swine in some areas of Zhejiang province.

Methods: Samples of mosquitoes and sera of swine were collected in three counties (Xianju, Longquan and Cixi) of Zhejiang province from May to October in 2007. 10 662 mosquitoes were collected during 2007, of which, C.

[Isolation and identification of Japanese encephalitis virus from mosquitoes in Zhejiang province].

Objective: To study the situation of arboviruses carried by mosquitoes in Zhejiang province.

Methods: Mosquitoes were collected from Zhejiang province in 2007. Virus strains were isolated by the inoculation of homogenates of the mosquitoes onto BHK-21 cell line.