Actein ameliorates diet-induced obesity through the activation of AMPK-mediated white fat browning.

Background: Targeting white adipose tissue (WAT) browning to increase systemic energy expenditure is a promising therapeutic strategy to combat obesity. Actein from Actaea cimicifuga L. has recently been reported to ameliorate high fat-induced hepatic steatosis.

Analgesic Buxus alkaloids with Enhanced Selectivity for the Low-Voltage-Gated Calcium Channel Ca 3.2 over Ca 3.1 through a New Binding Mode.

Low-voltage-gated calcium channels (LVGCCs; Ca 3.1-3.3) represent promising drug targets for epilepsy, pain, and essential tremor.

The Aqueous Extract of L. Exerts Phytotoxicity by Modulating HO and O Levels, Antioxidant Enzyme Activity and Phytohormone Levels.

Allelopathic interactions between plants serve as powerful tools for weed control. Despite the increasing understanding of the allelopathic mechanisms between different plant species, the inhibitory effects of on weed growth remain poorly understood. In this study, we conducted experiments to demonstrate that extract can suppress the germination of L.

Isolation and Identification of Herbicidal Active Compounds from L. and Exploration of the Binding Sites of Brassicanate A Sulfoxide.

L. has strong allelopathic effects on weeds. However, the allelochemicals with herbicidal activity in L.

Indole-3-acetonitrile Is a Critical Molecule with Weed Allopathic Suppression Function in Broccoli (Brassica oleracea var. italica).

Cruciferous plants are frequently used for ecologically benign weed control in agricultural production. Most effective Broccoli varieties were screened using the entropy method-based topsis model at first. Result showed that varieties of Lvwawa and Lvbaoshiwere most effective in allelopathic suppression on radishes.

Structurally modified Cyclovirobuxine-D Buxus alkaloids as effective analgesic agents through Ca3.2 T-Type calcium channel inhibition.

Cyclovirobuxine-D (CVB-D) is a Buxus alkaloid and a major active constituent in the Chinese medicinal herb Buxus microphylls. Traditionally, the natural alkaloid cyclovirobuxine-D has a long history of use as a traditional Chinese medicine for cardiovascular diseases as well as to treat a wide variety of medical conditions. As we found that CVB-D inhibited T-type calcium channels, we designed and synthesized a variety of fragments and analogues and evaluated them for the first time as new Ca3.

Self-powered photoelectrochemical aptasensor based on hollow tubular g-CN/Bi/BiVO for tobramycin detection.

A highly sensitive photoelectrochemical aptasensor based on phosphorus-doped hollow tubular g-CN/Bi/BiVO (PT-CN/Bi/BiVO) for tobramycin (TOB) detecting was developed. This aptasensor is a self-powered sensing system which could generate the electrical output under visible light irradiation with no external voltage supply. Based on the surface plasmon resonance (SPR) effect and unique hollow tubular structure of PT-CN/Bi/BiVO, the PEC aptasensor exhibited an enhanced photocurrent and favorably specific response to TOB.

Cyclovirobuxine D, a cardiovascular drug from traditional Chinese medicine, alleviates inflammatory and neuropathic pain mainly inhibition of voltage-gated Ca3.2 channels.

Cyclovirobuxine D (CVB-D), the main active constituent of traditional Chinese medicine , was developed as a safe and effective cardiovascular drug in China. has also been used to relieve various pain symptoms for centuries. In this study, we examined and uncovered strong and persistent analgesic effects of cyclovirobuxine D against several mouse models of pain, including carrageenan- and CFA-induced inflammatory pain and paclitaxel-mediated neuropathic hypersensitivity.

Caged polycyclic polyprenylated acylphloroglucinols as Ca3.2 low voltage-gated Ca channel inhibitors from .

Five caged polycyclic polyprenylated acylphloroglucinols including an unprecedented octahydro-2,5-methanoindene skeleton (1) were discovered from . Biologically, 1 and 2 are potent Ca3.2 T-type Ca channel inhibitors with negligible effect on the cardiovascular antitarget, the human ether-à-go-go-related gene potassium channel.

"Turn-off" photoelectrochemical aptasensor based on g-CN/WC/WO composites for tobramycin detection.

Tobramycin (TOB), as a widely used antibiotic, poses severe unpredictable risks to ecology and health. Here, a novel photoelectrochemical (PEC) aptasensor based on a "turn-off" PEC mode was constructed for TOB detection. The working electrode could be modified by g-CN/WC/WO composites and TOB aptamer probes in turn.

Icetexane diterpenoids as Ca3.2 T-type calcium channel inhibitors from Salvia prattii and analgesic effect of their Semi-synthesized derivatives.

Ten new icetexane diterpenoids, salpratins E-N (1-10) and a known analogue (11) were characterized from Salvia prattii Hemsl. Structurally, 1 is the first 19(4 → 3)-abeo-icetexane diterpenoid featuring with a 6/7/6 ring system. The structures of isolated compounds were determined by comprehensive analyses of spectroscopic data, ECD calculation, and single-crystal X-ray diffraction.

Pyranone Derivatives With Antitumor Activities, From the Endophytic Fungus sp. YN02-P-3.

Two new pyranone derivatives phomapyrone A (2) and phomapyrone B (3), one new coumarin 11 13-(+)-phomacumarin A (1), three known pyranones (4-6), together with three known amide alkaloids fuscoatramides A-C (7-9), as well as 9 11-(+)-ascosalitoxin (10) were isolated from the endophytic fungus sp. YN02-P-3, which was isolated from the healthy leaf tissue of a Paulownia tree in Yunnan Province, China. Their structures were elucidated using extensive NMR spectroscopic and HRESIMS data and by comparing the information with literature data.

Discovery of novel coffee diterpenoids with inhibitions on Ca3.1 low voltage-gated Ca channel.

Seven new (1-4, 6-8) diterpenoids with rare skeletons and seven known ones (9, 12, 17, 18 and 23-25) were isolated from roasted beans of Coffea arabica L. Together with previously obtained diterpenoids, a total of 26 molecules (1-25, 4a) were evaluated their activities on Ca3.1 low voltage-gated Ca channel.

Isoprenylated Flavonoids as Ca3.1 Low Voltage-Gated Ca Channel Inhibitors from Salvia digitaloides.

Saldigones A-C (1, 3, 4), three new isoprenylated flavonoids with diverse flavanone, pterocarpan, and isoflavanone architectures, were characterized from the roots of Salvia digitaloides, together with a known isoprenylated flavanone (2). Notably, it's the first report of isoprenylated flavonoids from Salvia species. The structures of these isolates were elucidated by extensive spectroscopic analysis.

Diterpenoid Alkaloids from the Aerial Parts of Aconitum flavum Hand.-Mazz.

Sixteen diterpenoid alkaloids (DAs), including six aconitine-type alkaloids (5 and 9 - 13), seven 7,17-seco-aconitine-type alkaloids (1 - 4, 6 - 8), two napelline-type alkaloids (14 and 15) as well as one veatchine-type alkaloid (16), were isolated from the aerial parts of Aconitum flavum Hand.-Mazz. In which, flavumolines A - D (1 - 4) were four new ones, and flavumoline E (5) was reported as natural compound for the first time.

New ent-kaurane diterpenes from the roasted arabica coffee beans and molecular docking to α-glucosidase.

Ten new (1-10) and five known (11-15) ent-kaurane diterpene derivatives were identified from the roasted beans of coffea arabica. Their structures were established by extensive spectroscopic analysis including 1D, 2D NMR (HSQC, HMBC, COSY, and ROESY), HRESIMS, and X-ray diffraction analysis. Compounds 1-3 were three types of rearranged ent-kaurane diterpenes, and compounds 4 and 5 were diterpene esters with a rare 6-hydroxyhexanoyl at C-17.

Novel Meroterpenoids from : Highly Potent Late Na1.5 Sodium Current Inhibitors.

Hypulatones A and B ( and ), two racemic meroterpenoids possessing an unprecedented spiro[benzofuran-2,1'-cycloundecan]-4'-ene-4,6(5)-dione core, were characterized from . Compound was found to significantly inhibit the late current of Na1.5 (late , IC = 0.

Structure and mechanism of human diacylglycerol O-acyltransferase 1.

Diacylglycerol O-acyltransferase 1 (DGAT1) synthesizes triacylglycerides and is required for dietary fat absorption and fat storage in humans. DGAT1 belongs to the membrane-bound O-acyltransferase (MBOAT) superfamily, members of which are found in all kingdoms of life and are involved in the acylation of lipids and proteins. How human DGAT1 and other mammalian members of the MBOAT family recognize their substrates and catalyse their reactions is unknown.

Congenetic Hybrids Derived from Dearomatized Isoprenylated Acylphloroglucinol with Opposite Effects on Ca3.1 Low Voltage-Gated Ca Channel.

A hybrid of dearomatized isoprenylated acylphloroglucinol (DIAP) and monoterpenoid, hypatone A (), together with its biosynthetic analogues - is characterized from . Structurally, possesses an unprecedented spiro[bicyclo[3.2.

Salpratlactones A and B: A Pair of - Tautomeric Abietanes as Ca3.1 T-Type Calcium Channel Agonists from .

Salpratlactones A () and B (), a pair of abietane - tautomers from , were identified as the first naturally occurring agonists of T-type calcium channel (TTCC). Structurally, and were featured by unique 6/5 carbocyclic rings bearing a γ-lactone ring through an exocyclic double bond. Moreover, both compounds and their mixture at 10 μM potently and equally increased Ca3.

Valepotriates From the Roots and Rhizomes of Jones as Novel N-Type Calcium Channel Antagonists.

The roots and rhizomes of have long been used as folk medicine in Asia and usually named as "Zhizhuxiang" in Chinese for the treatment of abdominal distention and pain. However, its active ingredients and molecular targets for treatment of abdominal pain remain unrevealed. Inhibitors of Ca2.

Structure of an EIIC sugar transporter trapped in an inward-facing conformation.

The phosphoenolpyruvate-dependent phosphotransferase system (PTS) transports sugar into bacteria and phosphorylates the sugar for metabolic consumption. The PTS is important for the survival of bacteria and thus a potential target for antibiotics, but its mechanism of sugar uptake and phosphorylation remains unclear. The PTS is composed of multiple proteins, and the membrane-embedded Enzyme IIC (EIIC) component transports sugars across the membrane.

Pepluanols C-D, Two Diterpenoids with Two Skeletons from Euphorbia peplus.

Pepluanols C and D (1 and 2), featuring unprecedented 5/5/10 with out,out-[7.2.1]bicylcododecane core and 6/6/7/3 fused-ring skeletons, respectively, were isolated from Euphorbia peplus.