Identification of a novel native peptide derived from 60S ribosomal protein L23a that translationally regulates p53 to reduce myocardial ischemia-reperfusion.

Myocardial ischemia-reperfusion (I/R) is a severe disease，but its underlying mechanism is not fully elucidated and no effective clinical treatment is available. Utilizing intracellular peptidomics, we identified a novel native peptide PDRL23A (Peptide Derived from RPL23A), that is intimately related to hypoxic stress. We further show that PDRL23A effectively alleviates hypoxia-induced cardiomyocyte injury in vitro, along with improvements in mitochondrial function and redox homeostasis, including ROS accumulation, oxidative phosphorylation, and mitochondrial membrane potential.

Rapid adaptation of brain-computer interfaces to new neuronal ensembles or participants via generative modelling.

For brain-computer interfaces (BCIs), obtaining sufficient training data for algorithms that map neural signals onto actions can be difficult, expensive or even impossible. Here we report the development and use of a generative model-a model that synthesizes a virtually unlimited number of new data distributions from a learned data distribution-that learns mappings between hand kinematics and the associated neural spike trains. The generative spike-train synthesizer is trained on data from one recording session with a monkey performing a reaching task and can be rapidly adapted to new sessions or monkeys by using limited additional neural data.

Characterization and optimization of the LAC4 upstream region for low-leakage expression in Kluyveromyces marxianus.

Kluyveromyces marxianus is a promising host for the production of heterologous proteins, chemicals, and bioethanol. One superior feature of this species is its capacity to assimilate lactose, which is rendered by the LAC12-LAC4 gene pair encoding a lactose permease and a β-galactosidase enzyme. Little is known about the regulation of LAC4 in K.

Comparing automated vs. manual data collection for COVID-specific medications from electronic health records.

Introduction: Data extraction from electronic health record (EHR) systems occurs through manual abstraction, automated extraction, or a combination of both. While each method has its strengths and weaknesses, both are necessary for retrospective observational research as well as sudden clinical events, like the COVID-19 pandemic. Assessing the strengths, weaknesses, and potentials of these methods is important to continue to understand optimal approaches to extracting clinical data.

Nonlinear resonance decomposition for weak signal detection.

This paper attempts to investigate the behaviors of coupling stochastic resonance (CSR) subject to α-stable noise and a periodic signal by using the residence-time ratio. Then, a nonlinear resonance decomposition is designed to successfully enhance and detect weak unknown multi-frequency signals embedded in strong α-stable noise by decomposing the noisy signal into a series of useful resonant components and a residue, where the residence-time ratio, instead of the output signal-to-noise ratio and other objective functions depending on the prior knowledge of the signals to be detected, can optimize the CSR to enhance weak unknown signals. Finally, the nonlinear resonance decomposition is used to process the raw vibration signal of rotating machinery.

A systematic review and meta-analysis of the mental health symptoms during the Covid-19 pandemic in Southeast Asia.

Aims: The Covid-19 pandemic has had a substantial impact on the mental health of the general public and high-risk groups worldwide. Due to its proximity and close links to China, Southeast Asia was one of the first regions to be affected by the outbreak. The aim of this systematic review was to evaluate the prevalence of anxiety, depression and insomnia in the general adult population and healthcare workers (HCWs) in Southeast Asia during the course of the first year of the pandemic.

Prevascularization Strategy with Three-Dimensional Porous Conduits for Neural Tissue Engineering.

Neovascularization is crucial for peripheral nerve regeneration and long-term functional restoration. Previous studies have emphasized strategies that enhance axonal repair over vascularization. Here, we describe the development and application of an prevascularization strategy that uses 3D porous nerve guidance conduits (NGCs) to achieve angiogenesis-mediated neural regeneration.

Nanoporous Graphene a Pressing Organization Calcination Strategy for Highly Efficient Electrocatalytic Hydrogen Peroxide Generation.

Nanoporous graphenes (NPGs) have recently attracted huge attention owing to their designable structures and diverse properties. Many important properties of NPGs are determined by their structural regularity and homogeneity. The mass production of NPGs with periodic well-defined pore structures under a solvent-free green synthesis poses a great challenge and is largely unexplored.

In situ deposition of nano CuO on electrospun chitosan nanofibrous scaffolds and their antimicrobial properties.

In order to obtain a synergistic antimicrobial effect of cuprous oxide nanoparticles (CuO NPs) and chitosan (CS) nanofibers, the nano CuO/CS nanofibrous scaffolds were synthesized in situ via two subsequent steps of chelation and reduction. The Cu were stably chelated on CS nanofibrous scaffolds through the coordination of amino group (-NH) and hydroxyl group (-OH) on CS with Cu, and then the chelated Cu were reduced to nano CuO by Vitamin C under alkaline conditions. And by the measurements of XRD, XPS and FTIR-ATR, the results showed that CuO NPs were successfully deposited on the CS nanofibrous scaffolds.

Capturing spike train temporal pattern with wavelet average coefficient for brain machine interface.

Motor brain machine interfaces (BMIs) directly link the brain to artificial actuators and have the potential to mitigate severe body paralysis caused by neurological injury or disease. Most BMI systems involve a decoder that analyzes neural spike counts to infer movement intent. However, many classical BMI decoders (1) fail to take advantage of temporal patterns of spike trains, possibly over long time horizons; (2) are insufficient to achieve good BMI performance at high temporal resolution, as the underlying Gaussian assumption of decoders based on spike counts is violated.

Observation of Efficacy of Internet-Based Chronic Disease Management Model Combined with Modified Therapy of Bushenyiliu Decoction in Treating Patients with Type 2 Diabetes Mellitus and Prostate Cancer and Its Effect on Disease Control Rate.

Objective: To explore the efficacy of Internet-based chronic disease management model combined with the modified therapy of Bushenyiliu decoction in treating patients with type 2 diabetes mellitus (T2DM) and prostate cancer and its effect on disease control rate (DCR).

Methods: 120 patients with T2DM and prostate cancer admitted to the Affiliated Hospital of Yangzhou University, Yangzhou First People's Hospital, from February 2019 to February 2020, were retrospectively analyzed and equally divided into the experimental group and the control group according to their admission order. Conventional treatment combined with the modified therapy of Bushenyiliu decoction was performed on all patients for 3 months, and the Internet-based chronic disease management model was adopted for patients in the experimental group additionally, so as to compare their short-term effect, survival time, disease progression, blood glucose indicators, immune function indicators, and type 2 Diabetes Self-Care Scale (2-DSCS) scores.

A Multidisciplinary Approach in Prenatal Diagnosis of TSC With Cardiac Rhabdomyoma as the Initial Symptom.

The long-term prognosis of a fetus with cardiac rhabdomyoma (CR) depends on the correlation with tuberous sclerosis complex (TSC). In recent years, the numerous variations of uncertain significance (VUS) of TSC genes produced by high-throughput sequencing have made counseling challenging, studies until now have tended to side-step the tricky topics. Here, we integrated detailed parental phenotype, echocardiography, neuro MRI, and genetic information to conduct a comprehensive evaluation of 61 CR fetuses.

Optical section structured illumination-based Förster resonance energy transfer imaging.

Förster resonance energy transfer (FRET) microscopy is an important tool suitable for studying molecular interactions in living cells. Optical section structured illumination microscopy (OS-SIM), like confocal microscopy, has about 200 nm spatial resolution. In this report, we performed quantitative 3-cube FRET imaging in OS-SIM mode and widefield microscopy (WF) mode, respectively, for living cells expressing FRET constructs consisting of Cerulean (C, donor) and Venus (V, acceptor).

Genomic analysis uncovers prognostic and immunogenic characteristics of ferroptosis for clear cell renal cell carcinoma.

In this study, the characteristic patterns of ferroptosis in clear cell renal cell carcinoma (ccRCC) were systematically investigated with the interactions between ferroptosis and the tumor microenvironment (TME). On the mRNA expression profiles of 57 ferroptosis-related genes (FRGs), three ferroptosis patterns were constructed, with distinct prognosis and immune cell infiltrations (especially T cells and dendritic cells). The high ferroptosis scores were characterized by poorer prognosis, increased T cell infiltration, higher immune and stromal scores, elevated tumor mutation burden, and enhanced response to anti-CTLA4 immunotherapy.

Risk Stratification of Early-Stage Cervical Cancer with Intermediate-Risk Factors: Model Development and Validation Based on Machine Learning Algorithm.

Background: Adjuvant therapy for patients with cervical cancer (CC) with intermediate-risk factors remains controversial. The objectives of the present study are to assess the prognoses of patients with early-stage CC with pathological intermediate-risk factors and to provide a reference for adjuvant therapy choice.

Materials And Methods: This retrospective study included 481 patients with stage IB-IIA CC.

K-80003 Inhibition of Macrophage Apoptosis and Necrotic Core Development in Atherosclerotic Vulnerable Plaques.

Purpose: Macrophage apoptosis coupled with a defective phagocytic clearance of the apoptotic cells promotes plaque necrosis in advanced atherosclerosis, which causes acute atherothrombotic vascular disease. Nonsteroidal anti-inflammatory drug sulindac derivative K-80003 treatment was previously reported to dramatically attenuate atherosclerotic plaque progression and destabilization. However, the underlying mechanisms are not fully understood.

Compounded with hemoglobin Port Phillip and -α or -- deletions were identified in Chinese population.

Introduction: Although over 1000 hemoglobin (Hb) variants were identified so far, Hb Port Phillip compound with α-thalassemia deletion had no reported before.

Methods: Two patients and the associated families from Guangdong province in China were recruited. Hematological parameters were determined by blood routine examination and hemoglobin electrophoresis.

Epidemiologic Study of Infection among Schoolchildren in the Republic of Marshall Islands.

The prevalence and risk factors of (pinworm) infection among primary schoolchildren (PSC) in the Marshall Islands remain unknown; thus, investigation on the status of pinworm infection rate is necessary to establish baseline data. After parents'/guardians' consent, a total of 346 children (179 boys and 167 girls) participated in this study. Individual's perianal area and thumbs were inspected by using the Scotch tape technique and cellophane tape method, respectively.

The complete mitochondrial genome of Meyrick, 1894 (Lepidoptera: Autostichidae).

The complete mitogenome of Meyrick, 1894 was determined in this study. It was 15,306 bps long and strongly AT biased. It consisted of 13 PCGs, 22 tRNAs, 2 rRNAs and 1 non-coding control region (371 bps).

The complete mitochondrial genome of (Wang 2003) (Lepidoptera: Autostichidae).

The mitogenome of was determined in this study. It was 15,268 bps long and strongly AT biased. It harbored 13 PCGs, 22 tRNAs, 2 rRNAs, and 1 non-coding control region (334 bps).

A Novel Multi-Exon Deletion of in a Three-Generation Pedigree: Supplements to Neurodevelopmental Disorder Spectrum.

neurodevelopmental disorder (-NDD) is a category of rare disorder characterized by intellectual disability, speech delay, dysmorphic facial features, and developmental delay. Other various physical abnormalities of -NDD might involve all organs and systems. Notably, there were only two unique missense mutations [c.

[Preimplantation Genetic Diagnosis of α/β Complex Thalassemia by Next Generation Sequencing].

Objective: To explore the application value of next generation sequencing (NGS) in preimplantation genetic diagnosis of α/β complex thalassemia couple.

Methods: The coding regions of α-globin genes (HBA1, HBA2) and β-globin gene (HBB) were selected as the target regions. The high-density and closely linked single nucleotide polymorphism (SNP) sites were selected as the genetic linkage markers in the upstream and downstream 2M regions of the gene.

GC-MS Based Metabolomics Reveals the Synergistic Mechanism of Gardeniae Fructus-Forsythiae Fructus Herb Pair in Lipopolysaccharide-Induced Acute Lung Injury Mouse Model.

Compatibility remains among the crucial and significant characteristics of traditional Chinese medicines. The Gardeniae Fructus (FG)-Forsythiae Fructus (FF) herb pair, an epitome of formulations for heat-clearing and detoxification, is extensively used to treat bacterial pneumonia in clinical settings. However, there are few reports on their synergistic effects.