Andrographolide suppresses cervical cancer progression by targeting angiogenesis and inducing apoptosis in a CAM-PDX model.

Cervical cancer poses significant clinical challenges, particularly in advanced stages. This study explores the therapeutic potential of andrographolide (AND), a bioactive compound derived from Andrographis paniculata, in mitigating cervical cancer progression using the chick embryo chorioallantoic membrane patient-derived xenograft (CAM-PDX) model. The model was validated through hematoxylin-eosin (H&E) staining and immunohistochemistry, which confirmed its ability to accurately replicate the histological and molecular characteristics of patient-derived xenografts (PDXs), establishing its reliability for therapeutic screening.

Effects of Polyol Types on Underwater Curing Properties of Polyurethane.

This study aims to develop castable polyurethane suitable for applications on wet substrates or underwater construction. Polyurethanes were synthesized using various polyols with similar hydroxyl values, including poly(tetrahydrofuran) polyol, polyester polyol, castor oil-modified polyol, soybean oil-modified polyol, and cashew nut shell oil-modified polyol. The corresponding polyurethane curing products were evaluated for their underwater curing characteristics by volume expansion ratios and adhesion strength on dry and wet substrates, combined with analyses of reaction exothermic behavior, wetting properties on dry and wet substrates, interfacial tension, and microstructure characterization from the perspectives of reaction activity and water solubility.

TagP, a PAAR-domain containing protein, plays roles in the fitness and virulence of .

Background: Type VI secretion system (T6SS) is widely present in Gram-negative bacteria and directly mediates antagonistic prokaryote interactions. PAAR (proline-alanine-alanine-arginine repeats) proteins have been proven essential for T6SS-mediated secretion and target cell killing. Although PAAR proteins are commonly found in , their biological functions are not fully disclosed yet.

An Integrated Gather-and-Distribute Mechanism and Attention-Enhanced Deformable Convolution Model for Pig Behavior Recognition.

The behavior of pigs is intricately tied to their health status, highlighting the critical importance of accurately recognizing pig behavior, particularly abnormal behavior, for effective health monitoring and management. This study addresses the challenge of accommodating frequent non-rigid deformations in pig behavior using deformable convolutional networks (DCN) to extract more comprehensive features by incorporating offsets during training. To overcome the inherent limitations of traditional DCN offset weight calculations, the study introduces the multi-path coordinate attention (MPCA) mechanism to enhance the optimization of the DCN offset weight calculation within the designed DCN-MPCA module, further integrated into the cross-scale cross-feature (C2f) module of the backbone network.

Development and evaluation of a multi-target droplet digital PCR assay for highly sensitive and specific detection of Yersinia pestis.

Background: Plague, caused by the bacterium Yersinia pestis, is a zoonotic disease that poses considerable threats to human health. Nucleic acid tests are crucial for plague surveillance and the rapid detection of Y. pestis.

Sex-specific impacts of thimerosal on the behaviors and brain monoaminergic systems in zebrafish (Danio rerio).

Thimerosal (THI) is the most widely used form of organic mercury in pharmaceutical and personal care products, and has become a major source of ethylmercury pollution in aquatic ecosystems. However, knowledge about its potential risk to aquatic species is limited. In this study, zebrafish were exposed to THI for 7 days, and variations in their behavioral traits, brain monoaminergic neurotransmitter contents, and related gene expression were investigated.

LINC00265 can Serve as a Potential Biomarker for Predicting Increased Disease Risk, Systemic Inflammation, Disease Severity and Poor Prognosis in Sepsis.

Background: Identifying effective therapeutic targets is of great significance for improving early diagnosis and prognosis of sepsis. This study aims to explore the role of LINC00265 in sepsis.

Methods: This is a retrospective study based on data collected from sepsis patients in 2017-2018.

Ethnobotanical Knowledge, Nutritional Composition, and Aroma Profile of Bailey: An Underutilized Wild Vegetable Endemic to China.

, an endemic species, serves as a wild and underutilized vegetable traditionally consumed in China. However, ethnobotanical and chemical studies of this species are not available. This study analyzed its associated ethnobotanical knowledge, nutritional composition and aroma profile.

Characterization of an aspartate aminotransferase encoded by YPO0623 with frequent nonsense mutations in .

, the causative agent of plague, is a genetically monomorphic bacterial pathogen that evolved from approximately 7,400 years ago. We observed unusually frequent mutations in YPO0623, mostly resulting in protein translation termination, which implies a strong natural selection. These mutations were found in all phylogenetic lineages of , and there was no apparent pattern in the spatial distribution of the mutant strains.

Regulatory Functions of PurR in : Orchestrating Diverse Biological Activities.

The bacterium has developed various strategies to sense and respond to the complex stresses encountered during its transmission and pathogenic processes. PurR is a common transcriptional regulator of purine biosynthesis among microorganisms, and it modulates the transcription level of the operon to suppress the production of hypoxanthine nucleotide (IMP). This study aims to understand the functions and regulatory mechanisms of in .

First Fossil Record of (Hymenophyllaceae) from the Mid-Cretaceous Kachin Amber, Myanmar.

Hymenophyllaceae (filmy ferns), with ca. 430 species, are the most species-rich family of early diverging leptosporangiate ferns but have a poor fossil record dating back to the Late Triassic period. Traditionally, Hymenophyllaceae comprise two species-rich genera or clades: (hymenophylloids) and () (trichomanoids).

Effectiveness of prophylactic pharmacological hemostatic agents for reduction of blood loss at vaginal surgery: a systematic review and meta-analysis.

Introduction And Hypothesis: The objective was to evaluate the effectiveness of pharmacological hemostatic agents in the reduction of blood loss at vaginal surgery.

Methods: A systematic review of randomized control trials (RCTs) was completed. We searched PubMed (1946-2022), Embase, and CINAHL, using search terms related to vaginal hysterectomies and reconstructive surgeries combined with peri-operative use of hemostatic agents.

SlbHLH152, a bHLH transcription factor positively regulates iron homeostasis in tomato.

The maintain of iron (Fe) homeostasis is essential for plant survival. In tomato, few transcription factors have been identified as regulators of Fe homeostasis, among which SlbHLH068 induced by iron deficiency, plays an important role. However, the upstream regulator(s) responsible for activating the expression of SlbHLH068 remain(s) unknown.

Palaeobotanical evidence reveals the living conditions of Miocene Lufengpithecus in East Asia.

Background: Understanding the relationship between human evolution and environmental changes is the key to lifting the veil on human origin. The hypothesis that environmental changes triggered the divergence of humans from apes (ca. 9.

Does embryo quality at transfer affect obstetric outcomes and placental findings?

Study Question: Do obstetric outcomes and placental findings in pregnancies conceived with IVF vary according to embryo quality?

Summary Answer: Pregnancies following the transfer of lower-quality embryos were associated with a higher rate of low-lying placentas and several adverse placental lesions.

What Is Known Already: A few studies have shown reduced pregnancy and live births rates with poor-quality embryo transfer, yet with comparable obstetric outcomes. None of these studies included placental analysis.

The effect of oestrogen dose and duration in programmed frozen cycles on obstetric outcomes and placental findings.

Research Question: Does programmed frozen embryo transfer (FET) with high-dose oestrogen affect obstetric outcomes and placental findings?

Design: A retrospective cohort of live singleton deliveries at a single institution between 2009 and 2017, including deliveries attained by IVF with programmed FET; oocyte recipients were excluded. High-dose oestrogen was defined as a daily dose >6 mg throughout treatment. All placentas were evaluated regardless of complication status and the Amsterdam classification was used to analyse findings.

Effect of Parity on Placental Histopathology Features in Singleton Live Births Following IVF.

The aim of this study was to evaluate the effect of parity (primipara vs multipara) on the histopathology of the placenta in singleton live births following in vitro fertilization. We conducted a retrospective cohort study evaluating data of all IVF resulted live births from one university affiliated hospital during 2009-2017. All patients had the placenta sent for pathological evaluation.

Diminished ovarian reserve is a risk factor for preeclampsia and placental malperfusion lesions.

Objective: To assess obstetric outcomes and placental findings in pregnancies attained by in vitro fertilization (IVF) in patients with diminished ovarian reserve (DOR).

Design: Retrospective cohort study.

Setting: University-affiliated tertiary hospital.

Does a history of recurrent pregnancy loss affect subsequent obstetric outcomes and placental findings in in vitro fertilization?

Purpose: To assess perinatal outcomes and placental findings in in vitro fertilization (IVF) patients with previous recurrent pregnancy loss (RPL).

Methods: This was a retrospective cohort of live singleton births following IVF at a single university-affiliated center between 2009 and 2017. Outcomes were compared between patients with previous RPL, defined as two miscarriages (RPL group), and patients without RPL (control group).

Pregnancy complications and placental histology in in vitro fertilization pregnancies with initial low serum β-hCG levels.

Objective: To assess perinatal outcomes and placental findings after in vitro fertilization (IVF) with an initial low serum β-human chorionic gonadotropin (hCG).

Design: A retrospective cohort study.

Setting: University-affiliated tertiary hospital.

The effect of endometriosis on placental histopathology and perinatal outcome in singleton live births resulting from IVF.

Research Question: Does endometriosis have an effect on the placental histopathology pattern and perinatal outcome in singleton live births resulting from IVF treatment?

Design: Retrospective cohort study evaluating the data on all live births following IVF treatment between 2009 and 2017 at one university-affiliated tertiary hospital. All patients had placentas sent for full gross and histopathology assessment, irrespective of complication status or delivery mode. The primary outcomes of the study included anatomical, inflammation, vascular malperfusion and villous maturation placental disorders.

The effect of higher estradiol levels during stimulation on pregnancy complications and placental histology.

Introduction: We aimed to assess obstetric outcomes and placental histology in stimulated in vitro fertilization (IVF) cycles with a high serum estradiol level.

Methods: This was a historic cohort of live singleton deliveries after IVF, at a single university affiliated medical center between 2009 and 2017. Included were pregnancies following controlled ovarian stimulation with fresh embryo transfer.

Pregnancy complications and placental histology following embryo transfer with a thinner endometrium.

Study Question: Are deliveries following IVF with a thinner endometrium associated with adverse perinatal outcomes and placental findings?

Summary Answer: Live births following IVF with a thinner endometrium are associated with an increased rate of placental-mediated obstetric complications and lower birthweight, while the placentas are notable for gross anatomical and histological malperfusion lesions.

What Is Known Already: Past studies have noted a higher rate of adverse outcomes on deliveries following IVF with a thinner endometrium, mainly placental-associated complications. However, no study to date has investigated placental histopathology in such cases.