Cold-seeps are areas of the ocean floor in which hydrogen sulfide and methane are released into the open water. The cold-seep microbes are an emerging source of novel bioactive natural products. Four new ansa-ring opened linear ansamycin analogues, named olimycins E-H (1-4) were isolated from the cold-seep-derived Streptomyces olivaceus OUCLQ19-3.
View Article and Find Full Text PDFGenomic bioinformatics analysis identified a bafilomycin biosynthetic gene cluster (named bfl) in the deepsea-derived S. samsunensis OUCT16-12, from which two new (1 and 2, named bafilomycins R and S) along with four known (3-6) bafilomycins were targetly obtained. The structure of 3 was clearly identified for the first time, thus named bafilomycin T herein.
View Article and Find Full Text PDFNine new (-, -, , and ; named filipins VI-XIV) and three known (, , and ) filipin-type polyene macrolides were isolated from the deep-sea-derived OUCT16-23 using a genome-guided strategy coupled with bioassay. Their structures were elucidated based on the extensive MS and NMR spectroscopic analyses together with ECD calculations. In an antifungal assay, compounds , , and - showed different degrees of growth inhibition against with minimum inhibitory concentrations (MICs) of 1.
View Article and Find Full Text PDFExploration of unstable compounds is a rarely explored area of natural product research. We describe the integration of genomic and metabolomic analyses with bioassay-guided compound mining to effectively explore unstable bacillaenes. New bacillaene structures (, , and ) were identified from compound mixtures using the DANS-SVI (ifferential nalysis of 2D MR pectrum-ingle spectrum with ariable ntensities) method, which were further verified by the isolation of the pure compounds under strictly controlled conditions.
View Article and Find Full Text PDF