The application of the inexpensive and synthetically simple electrocatalyst CuFe-MoC@NG in immunosensors.

In this study, we used inexpensive and synthetically simple electrocatalysts as replacements for conventional precious metal materials to reduce hydrogen peroxide (HO). We for the first time developed N-doped graphene-coated CuFe@MoC using one-step calcination of binary Prussian blue analogues (PBAs) with Mo cationic grafting precursors. The synergistic interaction of CuFe PBA and MoC increased the catalytically active sites for HO reduction.

Differences in Performance of Immunosensors Constructed Based on CeO-Simulating Auxiliary Enzymes.

The morphology effect of cerium oxide (CeO) has always been the focus of catalysis research. Few people have reported the relationship between the morphology of CeO and electrochemical performance in sensors. In this paper, a polyaniline (PANI) matrix is used as the dispersant and stabilizer, ultrafine Au nanoparticles (NPs) (Au@PANI) are uniformly embedded in the PANI matrix, and Au NPs@PANI is fixed on the surface of CeO with different morphologies and sizes (Spindle CeO:(SCe), octahedron CeO (OCe)).

Ultrasensitive electrochemical immunosensor based on the signal amplification strategy of the competitive reaction of Zn and ATP ions to construct a "signal on" mode GOx-HRP enzyme cascade reaction.

A GOx/HRP@ZIF-90 nanomaterial is proposed by coating GOx and HRP in ZIF-90 using a bio-simulated mineralization method to improve the tolerance of the enzyme to the external environment. In the detection process, the ZIF-90 is turned on under mild conditions by the competitive reaction of ATP with Zn and imidazole-2-carboxaldehyde (2-ICA), and the electrical signal of the system is amplified by the enzyme cascade reaction of GOx and HRP. Finally, based on the signal amplification strategy of the competitive reaction between Zn and ATP to construct a "signal on" mode, electrochemical immunosensor of GOx-HRP enzyme-linked cascade reaction was prepared.

A sandwich-type electrochemical immunosensor for ultrasensitive detection of multiple tumor markers using an electrical signal difference strategy.

Traditional sandwich-type electrochemical immunosensors can only detect single tumor markers because signal interference occurs when detecting multiple tumor markers. In this work, an electrical signal difference strategy was proposed for the accurate detection of multiple tumor markers. We labeled PdAgCeO mesoporous nanospheres with a carcinoembryonic antigen (CEA) secondary antibody and MnO nanosheets labeled with an alpha-fetoprotein (AFP) secondary antibody.

Bimetallic PtCu nanoparticles supported on molybdenum disulfide-functionalized graphitic carbon nitride for the detection of carcinoembryonic antigen.

A molybdenum disulfide based graphite phase carbon nitride (MoS/g-CN) which is supported by a platinum-copper nanoparticle (PtCu) Z-type catalyst was created in this study. The catalyst exploits optoelectronic synergistic effect with large surface area, good catalysis, and biocompatibility to amplify the signal. The electrode impedance of the synthesized MoS/g-CN-PtCu was reduced five times in visible light compared with dark conditions, thereby improving the detection of carcinoembryonic antigen (CEA).

Sensitive detection of carcinoembryonic antigen (CEA) by a sandwich-type electrochemical immunosensor using MOF-Ce@HA/Ag-HRP-Ab as a nanoprobe.

Sandwich-type electrochemical immunosensor was one of the main methods for detecting carcinoembryonic antigen (CEA). In this work, using Ce-MoF as the skeleton precursor, hyaluronic acid (HA) was coated on the surface of Ce-metal organic framework (Ce-MoF), which loaded with silver nanoparticles (Ag NPs) and horseradish peroxidase (HRP) to catalyze HO and double amplified the current signal. Thus, a sensitive sandwich-type electrochemical immunosensor (Ce-MoF@ HA/Ag-HRP) was designed to detect carcinoembryonic antigen (CEA).

A sandwich-type electrochemical immunosensor for detecting CEA based on CeO-MoS absorbed Pb.

A sandwich-type immunosensor for detecting the concentration of carcinoembryonic antigen (CEA) was prepared. In this work, gold nanoparticles (Au NPs) were used as platform to attach more primary antibody (Ab) due to excellent electrical conductivity and good biocompatibility. Molybdenum disulfide-Cerium oxide (CeO-MoS) nanohybrid was used as a carrier to absorb lead ions (Pb) and the second antibody (Ab).

Sandwich-type electrochemical immunosensor constructed using three-dimensional lamellar stacked CoS@C hollow nanotubes prepared by template-free method to detect carcinoembryonic antigen.

Effective treatment of cancer depends on early detection of tumor markers. In this paper, an effective template-free method was used to prepare CoS@C three-dimensional hollow sheet nanotubes as the matrix of the immunosensor. The unique three-dimensional hybrid hollow tubular nanostructure provides greater contact area and enhanced detection limit.

[Anaplastic lymphoma kinase-positive adenocarcinoma of lung: a cytopathologic analysis].

Objective: To study the cytomorphologic features of anaplastic lymphoma kinase (ALK)-rearranged pulmonary adenocarcinoma.

Methods: The morphologic features in 153 pulmonary adenocarcinoma cytology specimens encountered during the period from September, 2011 to April, 2015 in Shanghai Cancer Hospital were retrospectively reviewed. Fluorescence in-situ hybridization (FISH) and/or immunohistochemistry (Ventana D5F3) for ALK gene rearrangement were carried out.

[Cytogenetic analysis of childhood acute lymphoblastic leukemia].

Objective: To characterize the genetic aberrations in pediatric acute lymphoblastic leukemia (ALL).

Methods: Ninety ALL cases were enrolled in the study from January 2009 to November 2011. Chromosome banding analysis and fluorescence in situ hybridization (FISH) were used to detect genetic aberrations.

Osmotic stabilizer-coupled suppression of NDR defects is dependent on the calcium-calcineurin signaling cascade in Aspergillus nidulans.

Establishment and maintenance of cell polarity are coordinated by signaling pathways such as NDR (nuclear Dbf2-related) protein-kinase signaling and calcium signaling pathway. The NDR family of kinase is structurally related to the human myotonic dystrophy kinase, which, when impaired, confers a disease that involves changes in cytoarchitecture and ion homeostasis. CotA kinase, a member of the NDR protein kinase family, forms a complex with MobB to regulate cell polarized growth in Aspergillus nidulans.

Localization and function of calmodulin in live-cells of Aspergillus nidulans.

Calmodulin (CaM) is a small, eukaryotic protein that reversibly binds Ca(2+). Study of CaM localization in genetically tractable organisms has yielded many insights into CaM function. Here, we described the dynamic localization of Aspergillus nidulans CaM (AnCaM) in live-cells by using recombination strains with homologous, single cross-over insertions at the target gene which placed the GFP fused copy under the inducible alcA promoter and the RFP-CaM integration under the native cam promoter.