Unraveling the onset and continuity of depressive symptoms from early pregnancy to six weeks postpartum: A longitudinal network analysis.

Background: Perinatal depression is a major global public health problem, but the onset and progression of symptoms that give rise to perinatal depression are less clear in the scientific literature.

Methods: This study followed 1210 Chinese pregnant women who completed the Edinburgh Postnatal Depression Scale at five time points: 13, 24, and 37 weeks of gestation (G13, G24, G37), and 1 and 6 weeks postpartum (W1, W6). Cross-lagged panel networks were used to analyze and compare networks from first to second trimester (G13 → G24), second to third trimester (G24 → G37), and one week to six weeks postpartum (W1 → W6).

A Critical Role of Intracellular PD-L1 in Promoting Ovarian Cancer Progression.

Disrupting the interaction between tumor-cell surface PD-L1 and T cell membrane PD-1 can elicit durable clinical responses. However, only about 10% of ovarian cancer patients respond to PD-1/PD-L1 blockade. Here, we show that PD-L1 expression in ovarian cancer-patient tumors is predominantly intracellular.

Senescence-related cytokine levels are associated with HIV-1 serostatus and persistence.

Background: HIV-1 is associated with accelerated aging. The senescence-associated secretory phenotype (SASP) includes biological and cytokine profiles that induce cellular senescence and inflammaging. In this study, we leveraged the Multicenter AIDS Cohort Study (MACS) to evaluate the role of SASP in aging, HIV-1 reservoir, and inflammation in people with HIV-1 (PWH) on long-term suppressive antiretroviral therapy (ART).

Peripheral blood cell counts as predictors of immune-related adverse events in cancer patients receiving immune checkpoint inhibitors: a systematic review and meta-analysis.

Background: In recent years, immune checkpoint inhibitors (ICIs) have shown significant efficacy in treating various malignancies and have become a key therapeutic approach in cancer treatment. However, while ICIs activate the immune system, they can also induce immune-related adverse events (irAEs). Due to the variability in the frequency and severity of irAEs, clinical management faces a significant challenge in balancing antitumor efficacy with the risk of irAEs.

ThyroNet-X4 genesis: an advanced deep learning model for auxiliary diagnosis of thyroid nodules' malignancy.

Thyroid nodules are a common endocrine condition, and accurate differentiation between benign and malignant nodules is essential for making appropriate treatment decisions. Traditional ultrasound-based diagnoses often depend on the expertise of physicians, which introduces a risk of misdiagnosis. To address this challenge, this study proposes a novel deep learning model, ThyroNet-X4 Genesis, designed to automatically classify thyroid nodules as benign or malignant.

Modeling suggests SARS-CoV-2 rebound after nirmatrelvir-ritonavir treatment is driven by target cell preservation coupled with incomplete viral clearance.

Unlabelled: In a subset of SARS-CoV-2-infected individuals treated with the antiviral nirmatrelvir-ritonavir, the virus rebounds following treatment. The mechanisms driving this rebound are not well understood. We used a mathematical model to describe the longitudinal viral load dynamics of 51 individuals treated with nirmatrelvir-ritonavir, 20 of whom rebounded.

Hyperglycemic milieu impairs Vγ9Vδ2 T cell functions in tuberculosis patients and prolongs M.tb negative conversion time.

γδ T cells play protective roles in tuberculosis (TB). Our work demonstrated the therapeutic potential of allogeneic Vγ9Vδ2 T cells in TB patients. However, their functions in TB require further comprehensive evaluation.

Microstructure and Mechanical Properties of Mg-8Li-3Al-0.3Si Alloy Deformed Through a Combination of Back-Extrusion and Spinning Process.

In this work, the Mg-8Li-3Al-0.3Si (LAS830) alloy was prepared by the vacuum melting method. The as-cast alloy was subjected to backward extrusion at 250 °C and then spun at 250 °C.

Proteome-wide mendelian randomization identifies causal plasma proteins in interstitial lung disease.

Interstitial lung disease (ILD) has shown limited treatment advancements, with minimal exploration of circulating protein biomarkers causally linked to ILD and its subtypes beyond idiopathic pulmonary fibrosis (IPF). In this study, we aimed to identify potential drug targets and circulating protein biomarkers for ILD and its subtypes. We utilized the most recent large-scale plasma protein quantitative trait loci (pQTL) data detected from the antibody-based method and ILD and its subtypes' GWAS data from the updated FinnGen database for Mendelian randomization analysis.

A clinical analysis and literature review of eleven cases with primary pulmonary angiosarcoma.

Objective: The aim of this study is to explore the clinicopathological features, radiographic manifestations, treatment options, and prognosis of primary pulmonary angiosarcoma (PPAS).

Method: We summarized and analyzed the clinical data of 11 patients with primary pulmonary angiosarcoma treated at the First Affiliated Hospital of Guangzhou Medical University between January 2018 and January 2024. A retrospective analysis was conducted in conjunction with a review of the relevant literature.

Case report: Therapeutic response of front-line cadonilimab plus chemotherapy on patient with advanced lung adenocarcinoma harboring STK11 genetic aberration.

The STK11 gene mutation is a common genetic alteration in non-small cell lung cancer (NSCLC) and is significantly associated with poor responses to current immunotherapy regimens. Despite its prevalence, there is currently no established standard for front-line treatment in this subtype of NSCLC, underscoring the increasing need for personalized therapeutic strategies. In this report, we present a case of a patient with STK11-mutant NSCLC who was treated with first-line cadonilimab (10mg/kg) in combination with pemetrexed (500mg/m^2) plus carboplatin (AUC=5), resulting in a notable extension of progression-free survival (PFS).

Cross-neutralization of distant coronaviruses correlates with Spike S2-specific antibodies from immunocompetent and immunocompromised vaccinated SARS-CoV-2-infected patients.

As of May 2023, the public health emergency of COVID-19 was lifted across the globe. However, SARS-CoV-2 infections continue to be recorded worldwide. This situation has been attributed to the ability of the virus to evade host immune responses including neutralizing antibody-derived Immunity.

Physicochemical, structural properties, and in vitro digestibility of starches isolated from Amorphophallus konjac K. Koch and Amorphophallus dunnii: A comparison study.

This study compared the physicochemical and structural properties, and in vitro digestibility of Amorphophallus konjac K. Koch starch (AKS) and Amorphophallus dunnii starch (ADS), by using potato starch (PS) as a control. ADS exhibited larger granule sizes, which led to higher transparency, swelling power (SP), and water solubility than AKS.

Viral and immune predictors of HIV posttreatment control.

Purpose Of Review: This review focuses on the viral and immune factors influencing HIV posttreatment control (PTC), a rare condition where individuals maintain viral suppression after discontinuing antiretroviral therapy (ART).

Recent Findings: Studies demonstrate that early ART initiation leads to smaller HIV reservoirs and delayed viral rebound in PTCs. Virologically, PTCs harbor smaller HIV reservoirs and show lower levels of reservoir transcriptional activity compared with posttreatment noncontrollers.

Deacetylation enhances the structure and gelation properties of konjac glucomannan/soy protein isolate cold-set gels.

This study aimed to investigate the effect of the deacetylated konjac glucomannan (DKGM), with varying degree of deacetylation (DD), on the physicochemical and structural properties of transglutaminase-induced soy protein isolate (SPI) cold-set gels. Compared with native konjac glucomannan (KGM), DKGM significantly enhanced the gel strength, water-holding capacity, and thermal stability of the composite gels, with DK3 (DKGM with 65.85 % deacetylation) showing the most significant improvement.

Enhancing the gel properties of soy protein isolate: the role of oxidized konjac glucomannan in acid-induced gelation.

Background: Soy protein isolate (SPI) gels formed using a single coagulant often have poor water-holding capacity (WHC) and low hardness, making them fragile and unsuitable for transportation and storage. Adding compound coagulants or polysaccharides can improve the gelation properties of SPI gels induced by gluconolactone (GDL). This study explores the impact of oxidized konjac glucomannan (OKGM) on the physicochemical and structural properties of GDL-induced SPI gels, with the aim of evaluating the potential of OKGM for enhancing the overall quality and stability of these gels.

Modeling suggests SARS-CoV-2 rebound after nirmatrelvir-ritonavir treatment is driven by target cell preservation coupled with incomplete viral clearance.

In a subset of SARS-CoV-2 infected individuals treated with the oral antiviral nirmatrelvir-ritonavir, the virus rebounds following treatment. The mechanisms driving this rebound are not well understood. We used a mathematical model to describe the longitudinal viral load dynamics of 51 individuals treated with nirmatrelvir-ritonavir, 20 of whom rebounded.

Emerging SARS-CoV-2 Resistance After Antiviral Treatment.

Importance: Previous studies have identified mutations in SARS-CoV-2 strains that confer resistance to nirmatrelvir, yet how often this resistance arises and its association with posttreatment virologic rebound is not well understood.

Objective: To examine the prevalence of emergent antiviral resistance after nirmatrelvir treatment and its association with virologic rebound.

Design, Setting, And Participants: This cohort study enrolled outpatient adults with acute COVID-19 infection from May 2021 to October 2023.

Gender Difference in the Onset of Adolescent Depressive Symptoms: A Cross-Lagged Panel Network Analysis.

Depressive symptoms are prevalent in adolescents, especially girls, underscoring the need for early detection and targeted interventions. Identifying initial symptoms and their temporal associations is vital for such interventions. This study used cross-lagged panel network (CLPN) analysis to examine the central depressive symptoms and their interconnections within a national cohort derived from the China Family Panel Study (CFPS).

Pertussis upsurge, age shift and vaccine escape post-COVID-19 caused by ptxP3 macrolide-resistant Bordetella pertussis MT28 clone in China.

Objectives: China has experienced a notable upsurge in pertussis cases post-COVID-19, alongside an age shift to older children, increased vaccine escape, and a notable rise in the prevalence of macrolide-resistant Bordetella pertussis. Here, we present a genomic epidemiological investigation of these events.

Methods: We performed a retrospective observational study using culture-positive B pertussis isolated in Shanghai, China, from 2016 to 2024.

Strategies and Applications for Supramolecular Protein Self-Assembly.

Supramolecular chemistry achieves higher-order molecular self-assembly through non-covalent interactions. Utilizing supramolecular methods to explore the polymorphism of proteins, the building blocks of life, from a "bottom-up" perspective is essential for constructing diverse and functional biomaterials. In recent years, significant progress has been achieved in the design strategies and functional applications of supramolecular protein self-assembly, becoming a focal point for researchers.

A platform to deliver single and bi-specific Cas9/guide RNA to perturb genes in vitro and in vivo.

Although CRISPR-Cas9 technology is poised to revolutionize the treatment of diseases with underlying genetic mutations, it faces some significant issues limiting clinical entry. They include low-efficiency in vivo systemic delivery and undesired off-target effects. Here, we demonstrate, by modifying Cas9 with phosphorothioate-DNA oligos (PSs), that one can efficiently deliver single and bi-specific CRISPR-Cas9/guide RNA (gRNA) dimers in vitro and in vivo with reduced off-target effects.

Impact of Low-Frequency Human Immunodeficiency Virus Type 1 Drug Resistance Mutations on Antiretroviral Therapy Outcomes.

Background: The association between low-frequency human immunodeficiency virus type 1 (HIV-1) drug resistance mutations (DRMs) and treatment failure (TF) is controversial. We explore this association using next-generation sequencing (NGS) methods that accurately sample low-frequency DRMs.

Methods: We enrolled women with HIV-1 in Malawi who were either antiretroviral therapy (ART) naive (cohort A), had ART failure (cohort B), or had discontinued ART (cohort C).